Construct: ORF TRCN0000489096
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021845.1_s317c1
- DNA Barcode:
- ACCGTTTATCGGAGCAGTGTTCTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PPIB (5479)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489096
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5479 | PPIB | peptidylprolyl isomerase B | NM_000942.4 | 100% | 100% | |
| 2 | mouse | 19035 | Ppib | peptidylprolyl isomerase B | NM_011149.2 | 90.1% | 93.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 717
- ORF length:
- 648
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gctgcgcctc tccgaacgca acatgaaggt gctccttgcc gccgccctca 121 tcgcggggtc cgtcttcttc ctgctgctgc cgggaccttc tgcggccgat gagaagaaga 181 aggggcccaa agtcaccgtc aaggtgtatt ttgacctacg aattggagat gaagatgtag 241 gccgggtgat ctttggtctc ttcggaaaga ctgttccaaa aacagtggat aattttgtgg 301 ccttagctac aggagagaaa ggatttggct acaaaaacag caaattccat cgtgtaatca 361 aggacttcat gatccagggc ggagacttca ccaggggaga tggcacagga ggaaagagca 421 tcTACGGTGA GCGCTTCCCC GATGAGAACT TCAAACTGAA GCACTACGGG CCTGGCTGGG 481 TGAGCATGGC CAACGCAGGC AAAGACACCA ACGGCTCCCA GTTCTTCATC ACGACAGTCA 541 AGACAGCCTG GCTAGATGGC AAGCATGTGG TGTTTGGCAA AGTTCTAGAG GGCATGGAGG 601 TGGTGCGGAA GGTGGAGAGC ACCAAGACAG ACAGCCGGGA TAAACCCCTG AAGGATGTGA 661 TCATCGCAGA CTGCGGCAAG ATCGAGGTGG AGAAGCCCTT TGCCATCGCC AAGGAGTAGA 721 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 781 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 841 TTTATATATC TTGTGGAAAG GACGAACCGT TTATCGGAGC AGTGTTCTAA CGCGTTAAGT 901 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt