Construct: ORF TRCN0000489097
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021884.1_s317c1
- DNA Barcode:
- CTGTGGAAGGAACAAATTACATTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GABRR1 (2569)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489097
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2569 | GABRR1 | gamma-aminobutyric acid typ... | NM_002042.5 | 99.8% | 100% | 438C>T;1185G>A |
| 2 | human | 2569 | GABRR1 | gamma-aminobutyric acid typ... | NM_001256703.1 | 96.3% | 96.4% | 119_120ins51;387C>T;1134G>A |
| 3 | human | 2569 | GABRR1 | gamma-aminobutyric acid typ... | NM_001256704.1 | 81.6% | 81.8% | 0_1ins261;177C>T;924G>A |
| 4 | human | 2569 | GABRR1 | gamma-aminobutyric acid typ... | NM_001267582.1 | 81.6% | 81.8% | 0_1ins261;177C>T;924G>A |
| 5 | human | 2569 | GABRR1 | gamma-aminobutyric acid typ... | XM_017010689.1 | 81.6% | 81.8% | 0_1ins261;177C>T;924G>A |
| 6 | mouse | 14408 | Gabrr1 | gamma-aminobutyric acid (GA... | NM_008075.2 | 87.5% | 93.9% | (many diffs) |
| 7 | mouse | 14408 | Gabrr1 | gamma-aminobutyric acid (GA... | XM_006537613.3 | 79.8% | 83.7% | (many diffs) |
| 8 | mouse | 14408 | Gabrr1 | gamma-aminobutyric acid (GA... | XM_006537612.1 | 79.6% | 80.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1506
- ORF length:
- 1437
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gttggctgtc ccaaatatga gatttggcat ctttcttttg tggtggggat 121 gggttttggc cactgaaagc agaatgcact ggcccggaag agaagtccac gagatgtcta 181 agaaaggcag gccccaaaga caaagacgag aagtacatga agatgcccac aagcaagtca 241 gcccaattct gagacgaagt cctgacatca ccaaatcgcc tctgacaaag tcagaacagc 301 ttctgaggat agatgaccat gatttcagca tgaggcctgg ctttggaggc cctgccattc 361 ctgttggtgt ggatgtgcag gtggagagtt tggatagcat ctcagaggtt gacatggact 421 ttacgatgac cctctacctg aggcactact ggaaggacga gaggctgtct tttccaagca 481 ccaacaacct cagcatgacg tttgatggcc ggctggtcaa gaagatctgg gtccctgaca 541 tgtttttcgt gcactccaaa cgctccttca tccacgacac caccacagac aacgtcatgt 601 tgcgggtcca gcctgatggg aaagtgctct atagtctcag ggttacagta actgcaatgt 661 gcaacatgga cttcagccga tttcccttgg acacacaaac gtgctctctt gaaattgaaa 721 gctatgccta tacagaagat gacctcatgc tgtactggaa aaagggcaat gactccttaa 781 agacagatga acggatctca ctctcccagt tcctcattca ggaattccac accaccacca 841 aactggcttt ctacagcagc acaggctggt acaaccgtct ctacattaat ttcacgttgc 901 gtcgccacat cttcttcttc ttgctccaaa cttatttccc cgctaccctg atggtcatgc 961 tgtcctgggt gtccttctgg atcgaccgca gagccgtgcc tgccagagtc cccttaggta 1021 tcacaacggt gctgaccatg tccaccatca tcacgggcgt gaatgcctcc atgccgcgcg 1081 tctcctacat caaggccgtg gacatctacc tctgggtcag ctttgtgttc gtgttcctct 1141 cggtgctgga gtatgcggcc gtcaactacc tgaccactgt gcaggagagg aaggaacaga 1201 agctgcggga gaagcttccc tgcaccagcg gattaccTCC GCCCCGCACT GCAATGCTGG 1261 ACGGCAACTA CAGTGATGGG GAGGTGAATG ACCTGGACAA CTACATGCCA GAGAATGGAG 1321 AGAAGCCCGA CAGGATGATG GTGCAGCTGA CCCTGGCCTC AGAGAGGAGC TCCCCACAGA 1381 GGAAAAGTCA GAGAAGCAGC TATGTGAGCA TGAGAATCGA CACCCACGCC ATTGATAAAT 1441 ACTCCAGGAT CATCTTTCCA GCAGCATACA TTTTATTCAA TTTAATATAC TGGTCTATTT 1501 TCTCCTAGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1561 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1621 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTGTGG AAGGAACAAA TTACATTCAC 1681 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt