Construct: ORF TRCN0000489097

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021884.1_s317c1
DNA Barcode:
CTGTGGAAGGAACAAATTACATTC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
GABRR1 (2569)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489097
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2569 GABRR1 gamma-aminobutyric acid typ... NM_002042.5 99.8% 100% 438C>T;1185G>A
2 human 2569 GABRR1 gamma-aminobutyric acid typ... NM_001256703.1 96.3% 96.4% 119_120ins51;387C>T;1134G>A
3 human 2569 GABRR1 gamma-aminobutyric acid typ... NM_001256704.1 81.6% 81.8% 0_1ins261;177C>T;924G>A
4 human 2569 GABRR1 gamma-aminobutyric acid typ... NM_001267582.1 81.6% 81.8% 0_1ins261;177C>T;924G>A
5 human 2569 GABRR1 gamma-aminobutyric acid typ... XM_017010689.1 81.6% 81.8% 0_1ins261;177C>T;924G>A
6 mouse 14408 Gabrr1 gamma-aminobutyric acid (GA... NM_008075.2 87.5% 93.9% (many diffs)
7 mouse 14408 Gabrr1 gamma-aminobutyric acid (GA... XM_006537613.3 79.8% 83.7% (many diffs)
8 mouse 14408 Gabrr1 gamma-aminobutyric acid (GA... XM_006537612.1 79.6% 80.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1506
ORF length:
1437
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat gttggctgtc ccaaatatga gatttggcat ctttcttttg tggtggggat
121gggttttggc cactgaaagc agaatgcact ggcccggaag agaagtccac gagatgtcta
181agaaaggcag gccccaaaga caaagacgag aagtacatga agatgcccac aagcaagtca
241gcccaattct gagacgaagt cctgacatca ccaaatcgcc tctgacaaag tcagaacagc
301ttctgaggat agatgaccat gatttcagca tgaggcctgg ctttggaggc cctgccattc
361ctgttggtgt ggatgtgcag gtggagagtt tggatagcat ctcagaggtt gacatggact
421ttacgatgac cctctacctg aggcactact ggaaggacga gaggctgtct tttccaagca
481ccaacaacct cagcatgacg tttgatggcc ggctggtcaa gaagatctgg gtccctgaca
541tgtttttcgt gcactccaaa cgctccttca tccacgacac caccacagac aacgtcatgt
601tgcgggtcca gcctgatggg aaagtgctct atagtctcag ggttacagta actgcaatgt
661gcaacatgga cttcagccga tttcccttgg acacacaaac gtgctctctt gaaattgaaa
721gctatgccta tacagaagat gacctcatgc tgtactggaa aaagggcaat gactccttaa
781agacagatga acggatctca ctctcccagt tcctcattca ggaattccac accaccacca
841aactggcttt ctacagcagc acaggctggt acaaccgtct ctacattaat ttcacgttgc
901gtcgccacat cttcttcttc ttgctccaaa cttatttccc cgctaccctg atggtcatgc
961tgtcctgggt gtccttctgg atcgaccgca gagccgtgcc tgccagagtc cccttaggta
1021tcacaacggt gctgaccatg tccaccatca tcacgggcgt gaatgcctcc atgccgcgcg
1081tctcctacat caaggccgtg gacatctacc tctgggtcag ctttgtgttc gtgttcctct
1141cggtgctgga gtatgcggcc gtcaactacc tgaccactgt gcaggagagg aaggaacaga
1201agctgcggga gaagcttccc tgcaccagcg gattaccTCC GCCCCGCACT GCAATGCTGG
1261ACGGCAACTA CAGTGATGGG GAGGTGAATG ACCTGGACAA CTACATGCCA GAGAATGGAG
1321AGAAGCCCGA CAGGATGATG GTGCAGCTGA CCCTGGCCTC AGAGAGGAGC TCCCCACAGA
1381GGAAAAGTCA GAGAAGCAGC TATGTGAGCA TGAGAATCGA CACCCACGCC ATTGATAAAT
1441ACTCCAGGAT CATCTTTCCA GCAGCATACA TTTTATTCAA TTTAATATAC TGGTCTATTT
1501TCTCCTAGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1561CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1621TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTGTGG AAGGAACAAA TTACATTCAC
1681GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)