Construct: ORF TRCN0000489105

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021089.1_s317c1
DNA Barcode:
CAACGCTGACATACCCGTGCGCGG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
FZD3 (7976)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489105
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7976 FZD3 frizzled class receptor 3 NM_017412.4 99.9% 100% 435A>G
2 human 7976 FZD3 frizzled class receptor 3 NM_145866.1 99.9% 100% 435A>G
3 human 7976 FZD3 frizzled class receptor 3 XM_017013841.1 89.8% 89.9% 0_1ins201;234A>G
4 human 7976 FZD3 frizzled class receptor 3 XM_017013842.1 73.1% 71.1% (many diffs)
5 human 7976 FZD3 frizzled class receptor 3 XM_017013843.1 72% 70.7% (many diffs)
6 human 7976 FZD3 frizzled class receptor 3 XM_017013844.1 70.4% 70.2% (many diffs)
7 human 7976 FZD3 frizzled class receptor 3 XR_001745597.2 65.9% (many diffs)
8 human 7976 FZD3 frizzled class receptor 3 XR_949476.2 14.4% (many diffs)
9 mouse 14365 Fzd3 frizzled class receptor 3 NM_021458.2 92.1% 98.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2070
ORF length:
1998
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggctatg acttggattg tcttctctct ttggcccttg actgtgttca
121tggggcatat aggtgggcac agtttgtttt cttgtgaacc tattaccttg aggatgtgcc
181aagatttgcc ttataatact accttcatgc ctaatcttct gaatcattat gaccaacaga
241cagcagcttt ggcaatggag ccattccacc ctatggtgaa tctggattgt tctcgggatt
301tccggccttt tctttgtgca ctctacgctc ctatttgtat ggaatatgga cgtgtcacac
361ttccctgtcg taggctgtgt cagcgggctt acagtgagtg ttcgaagctc atggagatgt
421ttggtgttcc ttggcctgaa gatatggaat gcagtaggtt cccagattgt gatgagccat
481atcctcgact tgtggatctg aatttggctg gagaaccaac tgaaggagcc ccagtggcag
541tgcagagaga ctatggtttt tggtgtcccc gagagttaaa aattgatcct gatctgggtt
601attcttttct gcatgtgcgt gattgttcac ctccttgtcc aaatatgtac ttcagaagag
661aagaactgtc atttgctcgc tatttcatag gattgatttc aatcatttgc ctctcggcca
721cattgtttac ttttttaact tttttgattg atgtcacaag attccgttat cctgaaaggc
781ctattatatt ttatgcagtc tgctacatga tggtatcctt aattttcttc attggatttt
841tgcttgaaga tcgagtagcc tgcaatgcat ccatccctgc acaatataag gcttccacag
901tgacacaagg atctcataat aaagcctgta ccatgctttt tatgatactc tattttttta
961ctatggctgg cagtgtatgg tgggtaattc ttaccatcac atggttttta gcagctgtgc
1021caaagtgggg tagtgaagct attgagaaga aagcattgct gtttcacgcc agtgcatggg
1081gcatccccgg aactctaacc atcatccttt tagcgatgaa taaaattgaa ggtgacaata
1141ttagtggcgt gtgttttgtt ggcctctacg atgttgatgc attgagatat tttgttcttg
1201ctcccctctg cctgtatgtg gtagttgggg tttctctcct cttagctggc attatatccc
1261taaacagagt tcgaattgag attccattag aaaaggagaa ccaagataaa ttagtgaagt
1321ttatgatccg gatcggtgtt ttcagcattc tttatctcgt accactcttg gttgtaattg
1381gatgctactt ttatgagcaa gcttaccggg gcatctggga aacaacgtgg atacaagaac
1441gctgcagaga atatcacatt ccatgtccat atcaggttac tcaaatgagt cgtccagact
1501tgattctctt tctgatgaaa tacctgatgg ctctcatagt tggcattccc tctgtatttt
1561gggttggaag caaaaagaca tgctttgaat gggccagttt ttttcatggt cgtaggaaaa
1621aagagatagt gaatgagagc cgacaggtac tccaggaacc tgattttgct cagtctctcc
1681tgagggatcc aaatactcct atcataagaa agtcaagggG AACTTCCACT CAAGGAACAT
1741CCACCCATGC TTCTTCAACT CAGCTGGCTA TGGTGGATGA TCAAAGAAGC AAAGCAGGAA
1801GCATCCACAG CAAAGTGAGC AGCTACCACG GCAGCCTCCA CAGATCACGT GATGGCAGGT
1861ACACGCCCTG CAGTTACAGA GGAATGGAGG AGAGACTACC TCATGGCAGC ATGTCACGAC
1921TAACAGATCA CTCCAGGCAT AGTAGTTCTC ATCGGCTCAA TGAACAGTCA CGACATAGCA
1981GCATCAGAGA TCTCAGTAAT AATCCCATGA CTCATATCAC ACATGGCACC AGCATGAATC
2041GGGTTATTGA AGAAGATGGA ACCAGTGCTT AAGACCCAGC TTTCTTGTAC AAAGTGGTTG
2101ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
2161GTCCGTAACT TGAATATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACAAC
2221GCTGACATAC CCGTGCGCGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt
2281gtgaaagatt

Download FASTA (ORF) (Full)