Construct: ORF TRCN0000489114
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020687.1_s317c1
- DNA Barcode:
- GATTAAATTGAGTCTTAGATCCTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- COQ8A (56997)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489114
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56997 | COQ8A | coenzyme Q8A | NM_020247.5 | 99.9% | 100% | 1185C>T |
| 2 | human | 56997 | COQ8A | coenzyme Q8A | XM_005273201.1 | 99.9% | 100% | 1185C>T |
| 3 | human | 56997 | COQ8A | coenzyme Q8A | XM_011544238.1 | 99.9% | 100% | 1185C>T |
| 4 | human | 56997 | COQ8A | coenzyme Q8A | XM_011544239.2 | 99.9% | 100% | 1185C>T |
| 5 | human | 56997 | COQ8A | coenzyme Q8A | XM_011544241.2 | 99.9% | 100% | 1185C>T |
| 6 | human | 56997 | COQ8A | coenzyme Q8A | XM_017001852.1 | 99.9% | 100% | 1185C>T |
| 7 | human | 56997 | COQ8A | coenzyme Q8A | XM_024448517.1 | 99.9% | 100% | 1185C>T |
| 8 | human | 56997 | COQ8A | coenzyme Q8A | XM_024448518.1 | 99.9% | 100% | 1185C>T |
| 9 | mouse | 67426 | Coq8a | coenzyme Q8A | NM_001163290.1 | 83.4% | 86.5% | (many diffs) |
| 10 | mouse | 67426 | Coq8a | coenzyme Q8A | NM_023341.3 | 83.4% | 86.5% | (many diffs) |
| 11 | mouse | 67426 | Coq8a | coenzyme Q8A | XM_006496961.2 | 83.4% | 86.5% | (many diffs) |
| 12 | mouse | 67426 | Coq8a | coenzyme Q8A | XM_006496962.2 | 83.4% | 86.5% | (many diffs) |
| 13 | mouse | 67426 | Coq8a | coenzyme Q8A | XM_006496963.3 | 77.8% | 79.9% | (many diffs) |
| 14 | mouse | 67426 | Coq8a | coenzyme Q8A | XM_011238855.2 | 67.2% | 71.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2013
- ORF length:
- 1941
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggctgcc atattgggag acaccatcat ggtggctaaa ggccttgtca 121 agctgaccca ggcggccgtg gaaacccacc tgcagcactt gggcatcgga ggggagctga 181 tcatggcggc cagggccctg cagtccacgg ctgtggagca gattggcatg ttcttgggga 241 aggtgcaggg tcaggataaa catgaagaat attttgctga gaacttcggc ggcccagaag 301 gggagttcca cttctcagtc ccgcatgcag ccggagcctc cacagacttc tcttcagcct 361 ccgctcccga ccagtcagcg cccccatccc tgggtcatgc ccacagcgag ggcccagctc 421 ctgcctacgt ggccagtgga ccctttagag aagccgggtt ccccggccag gcctcctccc 481 ctctgggcag ggccaacggg aggctctttg caaaccccag agactcattc tctgccatgg 541 gctttcagcg aaggttcttc caccaggacc aatcccctgt tgggggcctc acagccgagg 601 acattgagaa ggcccggcag gctaaggctc gccccgagaa caagcagcac aaacagacgc 661 tcagcgagca tgcccgggag cggaaggtgc ctgtgacgag gattggccgg ctggccaact 721 tcggaggtct ggccgtgggc ctgggcttcg gggcactggc agaggtcgcc aagaagagcc 781 tgcgctccga ggacccctca gggaagaagg ccgtgctggg ttccagtcct ttcctgtccg 841 aggccaatgc agagcggatc gtgcgcacgc tctgcaaggt gcgtggtgcg gcactcaagc 901 tgggccagat gctgagcatc caggatgatg cctttatcaa cccccacctg gctaagatct 961 tcgagcgggt gcggcagagc gcggacttca tgccactgaa gcagatgatg aaaactctca 1021 acaacgacct gggccccaac tggcgggaca agttggaata cttcgaggag cggcccttcg 1081 ccgccgcatc cattgggcag gtgcacttgg cccgaatgaa gggcggccgc gaggtggcca 1141 tgaagatcca gtaccctggc gtggcccaga gcatcaacag tgatgtcaac aacctcatgg 1201 ccgtgttgaa catgagcaac atgcttccag aaggcctgtt ccccgagcac ctgattgacg 1261 tgctgaggcg ggagctggcc ctggagtgtg actaccagcg agaggccgcc tgtgcccgca 1321 agttcaggga cctgctgaag ggccacccct tcttctatgt gcctgagatt gtggatgagc 1381 tctgcagccc acatgtgctg accacagagc tggtgtctgg cttccccctg gaccaggccg 1441 aagggctcag ccaggagatt cggaacgaga tctgctacaa catcctggtt ctgtgcctga 1501 gggagctgtt cgagttccac ttcatgcaaa cagaccccaa ctggtccaac ttcttctatg 1561 acccccagca gcacaaggtg gctcttttgg attttggggc aacgcgggaa tatgacagat 1621 ccttcaccga cctctacatt cagatcatca gggctgctgc cgacagggac agggagactg 1681 tgcgggcgaa atccatagag atgaagttcc tcaccggcta cgaggtcaag gtcatggaag 1741 acgcccactt ggatgccatc ctcatcctgg gggaggcctt cgccTCTGAT GAGCCTTTTG 1801 ATTTTGGCAC TCAGAGCACC ACCGAGAAGA TCCACAACCT GATTCCCGTC ATGCTGAGGC 1861 ACCGTCTCGT CCCCCCACCC GAGGAAACCT ACTCCCTGCA CAGGAAGATG GGGGGCTCCT 1921 TCCTCATCTG CTCCAAGCTG AAGGCCCGCT TCCCCTGCAA GGCCATGTTC GAGGAGGCCT 1981 ACAGCAACTA CTGCAAGAGG CAGGCCCAGC AGTAGAACCC AGCTTTCTTG TACAAAGTGG 2041 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2101 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2161 AGATTAAATT GAGTCTTAGA TCCTTACGCG TTAAGTCgac aatcaacctc tggattacaa 2221 aatttgtgaa agatt