Construct: ORF TRCN0000489124
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020220.1_s317c1
- DNA Barcode:
- TAATCGTGGCGACGGTAACATTCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PFKL (5211)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489124
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5211 | PFKL | phosphofructokinase, liver ... | NM_002626.6 | 99.9% | 99.8% | 366C>T;1982T>C |
| 2 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_005261135.3 | 97% | 97% | 159_227del;435C>T;2051T>C |
| 3 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_017028369.1 | 96.8% | 96.4% | (many diffs) |
| 4 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_005261137.4 | 96.1% | 96.1% | 0_1ins87;279C>T;1895T>C |
| 5 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_017028370.1 | 96.1% | 96.1% | 0_1ins87;279C>T;1895T>C |
| 6 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_017028372.1 | 96.1% | 96.1% | 0_1ins87;279C>T;1895T>C |
| 7 | human | 5211 | PFKL | phosphofructokinase, liver ... | NM_001002021.3 | 92.1% | 91.3% | (many diffs) |
| 8 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_024452085.1 | 92.1% | 91.3% | (many diffs) |
| 9 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_006724011.1 | 89.7% | 88.8% | (many diffs) |
| 10 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_017028368.1 | 89.7% | 88.8% | (many diffs) |
| 11 | human | 5211 | PFKL | phosphofructokinase, liver ... | XM_011529603.2 | 70.3% | 67.8% | (many diffs) |
| 12 | mouse | 18641 | Pfkl | phosphofructokinase, liver,... | NM_008826.4 | 87.1% | 94.1% | (many diffs) |
| 13 | mouse | 18641 | Pfkl | phosphofructokinase, liver,... | XM_017313846.1 | 52.1% | 55.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2412
- ORF length:
- 2340
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccgcg gtggacctgg agaagctgcg ggcgtcgggc gcgggcaagg 121 ccatcggcgt cctgaccagc ggcggcgacg cgcaaggcat gaacgctgct gtccgggctg 181 tgacgcgcat gggcatttat gtgggtgcca aagtcttcct catctacgag ggctatgagg 241 gcctcgtgga gggaggtgag aacatcaagc aggccaactg gctgagcgtc tccaacatca 301 tccagctggg cggcactatc attggcagcg ctcgctgcaa ggcctttacc accagggagg 361 ggcgccgggc agcggcctac aacctggtcc agcacggcat caccaacctg tgcgtcatcg 421 gcggggatgg cagccttaca ggtgccaaca tcttccgcag cgagtggggc agcctgctgg 481 aggagctggt ggcggaaggt aagatctcag agactacagc ccggacctac tcgcacctga 541 acatcgcggg cctagtgggc tccatcgata acgacttctg cggcaccgac atgaccatcg 601 gcacggactc ggccctccac cgcatcatgg aggtcatcga tgccatcacc accactgccc 661 agagccacca gaggaccttc gtgctggaag tgatgggccg gcactgcggg tacctggcgc 721 tggtatctgc actggcctca ggggccgact ggctgttcat ccccgaggct ccacccgagg 781 acggctggga gaacttcatg tgtgagaggc tgggtgagac tcggagccgt gggtcccgac 841 tgaacatcat catcatcgct gagggtgcca ttgaccgcaa cgggaagccc atctcgtcca 901 gctacgtgaa ggacctggtg gttcagaggc tgggcttcga cacccgtgta actgtgctgg 961 gccacgtgca gcggggaggg acgccctctg ccttcgaccg gatcctgagc agcaagatgg 1021 gcatggaggc ggtgatggcg ctgctggaag ccacgcctga cacgccggcc tgcgtggtca 1081 ccctctcggg gaaccagtca gtgcggctgc ccctcatgga gtgcgtgcag atgaccaagg 1141 aagtgcagaa agccatggat gacaagaggt ttgacgaggc cacccagctc cgtggtggga 1201 gcttcgagaa caactggaac atttacaagc tcctcgccca ccagaagccc cccaaggaga 1261 agtctaactt ctccctggcc atcctgaatg tgggggcccc ggcggctggc atgaatgcgg 1321 ccgtgcgctc ggcggtgcgg accggcatct cccatggaca cacagtatac gtggtgcacg 1381 atggcttcga aggcctagcc aagggtcagg tgcaagaagt aggctggcac gacgtggccg 1441 gctggttggg gcgtggtggc tccatgctgg ggaccaagag gaccctgccc aagggccagc 1501 tggagtccat tgtggagaac atccgcatct atggtattca cgccctgctg gtggtcggtg 1561 ggtttgaggc ctatgaaggg gtgctgcagc tggtggaggc tcgcgggcgc tacgaggagc 1621 tctgcatcgt catgtgtgtc atcccagcca ccatcagcaa caacgtccct ggcaccgact 1681 tcagcctggg ctccgacact gctgtaaatg ccgccatgga gagctgtgac cgcatcaaac 1741 agtctgcctc ggggaccaag cgccgtgtgt tcatcgtgga gaccatgggg ggttactgtg 1801 gctacctggc caccgtgact ggcattgctg tgggggccga cgccgcctac gtcttcgagg 1861 accctttcaa catccacgac ttaaaggtca acgtggagca catgacggag aagatgaaga 1921 cagacattca gaggggcctg gtgctgcgga acgagaagtg ccatgactac tacaccacgg 1981 agttcctgta caacctgtac tcatcagagg gcaagggcgt cttcgactgc aggaccaatg 2041 tcctgggcca cccgcagcag ggtggcgcTC CAACCCCCTT TGACCGGAAC TATGGGACCA 2101 AGCTGGGGGT GAAGGCCATG CTGTGGTTGT CGGAGAAGCT GCGCGAGGTT TACCGCAAGG 2161 GACGGGTGTT CGCCAATGCC CCAGACTCGG CCTGCGTGAT CGGCCTGAAG AAGAAGGCGG 2221 TGGCCTTCAG CCCCGTCACT GAGCTCAAGA AAGACACTGA TTTCGAGCAC CGCATGCCAC 2281 GGGAGCAGTG GTGGCTGAGC CTGCGGCTCA TGCTGAAGAT GCTGGCACAA TACCGCATCA 2341 GTATGGCCGC CTACGTGTCA GGGGAGCTGG AGCACGTGAC CCGCCGCACC CTGAGCATGG 2401 ACAAGGGCTT CTGAGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2461 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAATAT 2521 TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT AATCGTGGCG ACGGTAACAT 2581 TCGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att