Construct: ORF TRCN0000489130

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020074.1_s317c1
DNA Barcode:
GCCTAGCCTAATGAGAGCAGCGAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HIF1A (3091)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489130
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3091 HIF1A hypoxia inducible factor 1 ... NM_001530.4 99.9% 99.8% 2478_2479insG
2 human 3091 HIF1A hypoxia inducible factor 1 ... NM_001243084.1 95.8% 96% (many diffs)
3 human 3091 HIF1A hypoxia inducible factor 1 ... NM_181054.3 88.9% 88.7% 2202_2203insGGAAC;2205_2206ins269
4 mouse 15251 Hif1a hypoxia inducible factor 1,... XM_017314961.1 88.7% 90.4% (many diffs)
5 mouse 15251 Hif1a hypoxia inducible factor 1,... XM_006515478.3 88.6% 90.2% (many diffs)
6 mouse 15251 Hif1a hypoxia inducible factor 1,... NM_001313920.1 87.3% 89.2% (many diffs)
7 mouse 15251 Hif1a hypoxia inducible factor 1,... NM_010431.2 87.2% 88.9% (many diffs)
8 mouse 15251 Hif1a hypoxia inducible factor 1,... NM_001313919.1 87.1% 88.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2550
ORF length:
2481
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat ggagggcgcc ggcggcgcga acgacaagaa aaagataagt tctgaacgtc
121gaaaagaaaa gtctcgagat gcagccagat ctcggcgaag taaagaatct gaagtttttt
181atgagcttgc tcatcagttg ccacttccac ataatgtgag ttcgcatctt gataaggcct
241ctgtgatgag gcttaccatc agctatttgc gtgtgaggaa acttctggat gctggtgatt
301tggatattga agatgacatg aaagcacaga tgaattgctt ttatttgaaa gccttggatg
361gttttgttat ggttctcaca gatgatggtg acatgattta catttctgat aatgtgaaca
421aatacatggg attaactcag tttgaactaa ctggacacag tgtgtttgat tttactcatc
481catgtgacca tgaggaaatg agagaaatgc ttacacacag aaatggcctt gtgaaaaagg
541gtaaagaaca aaacacacag cgaagctttt ttctcagaat gaagtgtacc ctaactagcc
601gaggaagaac tatgaacata aagtctgcaa catggaaggt attgcactgc acaggccaca
661ttcacgtata tgataccaac agtaaccaac ctcagtgtgg gtataagaaa ccacctatga
721cctgcttggt gctgatttgt gaacccattc ctcacccatc aaatattgaa attcctttag
781atagcaagac tttcctcagt cgacacagcc tggatatgaa attttcttat tgtgatgaaa
841gaattaccga attgatggga tatgagccag aagaactttt aggccgctca atttatgaat
901attatcatgc tttggactct gatcatctga ccaaaactca tcatgatatg tttactaaag
961gacaagtcac cacaggacag tacaggatgc ttgccaaaag aggtggatat gtctgggttg
1021aaactcaagc aactgtcata tataacacca agaattctca accacagtgc attgtatgtg
1081tgaattacgt tgtgagtggt attattcagc acgacttgat tttctccctt caacaaacag
1141aatgtgtcct taaaccggtt gaatcttcag atatgaaaat gactcagcta ttcaccaaag
1201ttgaatcaga agatacaagt agcctctttg acaaacttaa gaaggaacct gatgctttaa
1261ctttgctggc cccagccgct ggagacacaa tcatatcttt agattttggc agcaacgaca
1321cagaaactga tgaccagcaa cttgaggaag taccattata taatgatgta atgctcccct
1381cacccaacga aaaattacag aatataaatt tggcaatgtc tccattaccc accgctgaaa
1441cgccaaagcc acttcgaagt agtgctgacc ctgcactcaa tcaagaagtt gcattaaaat
1501tagaaccaaa tccagagtca ctggaacttt cttttaccat gccccagatt caggatcaga
1561cacctagtcc ttccgatgga agcactagac aaagttcacc tgagcctaat agtcccagtg
1621aatattgttt ttatgtggat agtgatatgg tcaatgaatt caagttggaa ttggtagaaa
1681aactttttgc tgaagacaca gaagcaaaga acccattttc tactcaggac acagatttag
1741acttggagat gttagctccc tatatcccaa tggatgatga cttccagtta cgttccttcg
1801atcagttgtc accattagaa agcagttccg caagccctga aagcgcaagt cctcaaagca
1861cagttacagt attccagcag actcaaatac aagaacctac tgctaatgcc accactacca
1921ctgccaccac tgatgaatta aaaacagtga caaaagaccg tatggaagac attaaaatat
1981tgattgcatc tccatctcct acccacatac ataaagaaac tactagtgcc acatcatcac
2041catatagaga tactcaaagt cggacagcct caccaaacag agcaggaaaa ggagtcatag
2101aacagacaga aaaatctcat ccaagaagcc ctaacgtgtt atctgtcgct ttgagtcaaa
2161gaactacagt tcctgaggaa gaactaaatc caaagatact agctttgcag aatgctcaga
2221gaaagcgaaa aatggaacat gatggttcac tttttcaagc agtaggaatt ggaacattat
2281tacagcagcc agacgatcat gcagctacta catcactttc ttGGAAACGT GTAAAAGGAT
2341GCAAATCTAG TGAACAGAAT GGAATGGAGC AAAAGACAAT TATTTTAATA CCCTCTGATT
2401TAGCATGTAG ACTGCTGGGG CAATCAATGG ATGAAAGTGG ATTACCACAG CTGACCAGTT
2461ATGATTGTGA AGTTAATGCT CCTATACAAG GCAGCAGAAA CCTACTGCAG GGTGAAGAAT
2521TACTCAGAGC TTTGGATCAA GTTAACGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG
2581GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
2641AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCCTAGCC
2701TAATGAGAGC AGCGATACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
2761aagatt

Download FASTA (ORF) (Full)

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