Construct: ORF TRCN0000489136
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019361.1_s317c1
- DNA Barcode:
- TACCTGTCTAAGAGGGGCAGCTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LPAR3 (23566)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489136
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23566 | LPAR3 | lysophosphatidic acid recep... | NM_012152.3 | 99.9% | 99.7% | 1059_1060insG |
| 2 | human | 23566 | LPAR3 | lysophosphatidic acid recep... | XM_024446128.1 | 99.9% | 99.7% | 1059_1060insG |
| 3 | human | 23566 | LPAR3 | lysophosphatidic acid recep... | XM_024446127.1 | 96.8% | 96.7% | 1_33del;1092_1093insG |
| 4 | mouse | 65086 | Lpar3 | lysophosphatidic acid recep... | NM_022983.4 | 84.6% | 90.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1137
- ORF length:
- 1062
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgaat gagtgtcact atgacaagca catggacttt ttttataata 121 ggagcaacac tgatactgtc gatgactgga caggaacaaa gcttgtgatt gttttgtgtg 181 ttgggacgtt tttctgcctg tttatttttt tttctaattc tctggtcatc gcggcagtga 241 tcaaaaacag aaaatttcat ttccccttct actacctgtt ggctaattta gctgctgccg 301 atttcttcgc tggaattgcc tatgtattcc tgatgtttaa cacaggccca gtttcaaaaa 361 ctttgactgt caaccgctgg tttctccgtc aggggcttct ggacagtagc ttgactgctt 421 ccctcaccaa cttgctggtt atcgccgtgg agaggcacat gtcaatcatg aggatgcggg 481 tccatagcaa cctgaccaaa aagagggtga cactgctcat tttgcttgtc tgggccatcg 541 ccatttttat gggggcggtc cccacactgg gctggaattg cctctgcaac atctctgcct 601 gctcttccct ggcccccatt tacagcagga gttaccttgt tttctggaca gtgtccaacc 661 tcatggcctt cctcatcatg gttgtggtgt acctgcggat ctacgtgtac gtcaagagga 721 aaaccaacgt cttgtctccg catacaagtg ggtccatcag ccgccggagg acacccatga 781 agctaatgaa gacggtgatg actgtcttag ggGCGTTTGT GGTATGCTGG ACCCCGGGCC 841 TGGTGGTTCT GCTCCTCGAC GGCCTGAACT GCAGGCAGTG TGGCGTGCAG CATGTGAAAA 901 GGTGGTTCCT GCTGCTGGCG CTGCTCAACT CCGTCGTGAA CCCCATCATC TACTCCTACA 961 AGGACGAGGA CATGTATGGC ACCATGAAGA AGATGATCTG CTGCTTCTCT CAGGAGAACC 1021 CAGAGAGGCG TCCCTCTCGC ATCCCCTCCA CAGTCCTCAG CAGGAGTGAC ACAGGCAGCC 1081 AGTACATAGA GGATAGTATT AGCCAAGGTG CAGTCTGCAA TAAAAGCACT TCCGACCCAG 1141 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1201 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1261 TATCTTGTGG AAAGGACGAT ACCTGTCTAA GAGGGGCAGC TTAACGCGTT AAGTCgacaa 1321 tcaacctctg gattacaaaa tttgtgaaag att