Construct: ORF TRCN0000489156
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020529.1_s317c1
- DNA Barcode:
- ACCAGTCTCTATTACCGGTCTAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NME5 (8382)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489156
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8382 | NME5 | NME/NM23 family member 5 | NM_003551.3 | 100% | 100% | |
| 2 | human | 8382 | NME5 | NME/NM23 family member 5 | XM_017009945.2 | 72.4% | 67.9% | (many diffs) |
| 3 | human | 8382 | NME5 | NME/NM23 family member 5 | XM_024446227.1 | 72.4% | 67.9% | (many diffs) |
| 4 | human | 8382 | NME5 | NME/NM23 family member 5 | XM_024446228.1 | 69.8% | 68.3% | (many diffs) |
| 5 | human | 8382 | NME5 | NME/NM23 family member 5 | XM_005272099.2 | 69.4% | 68.3% | 437A>T;440_440delAinsTT;444_445ins191 |
| 6 | mouse | 75533 | Nme5 | NME/NM23 family member 5 | NM_080637.3 | 83.3% | 85.8% | (many diffs) |
| 7 | mouse | 75533 | Nme5 | NME/NM23 family member 5 | XM_017318004.1 | 83.3% | 85.8% | (many diffs) |
| 8 | mouse | 75533 | Nme5 | NME/NM23 family member 5 | XM_006526319.2 | 75.1% | 72.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 708
- ORF length:
- 636
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagata tcaatgcctc cacctcagat atatgtagaa aaaactctgg 121 ccattatcaa accagatatt gttgacaaag aggaggagat acaagatatt attcttagat 181 ccggattcac cattgttcag agaagaaaac tacgcctcag ccctgagcaa tgtagtaact 241 tttatgtgga aaagtatgga aaaatgtttt tccccaactt aacagcttac atgagttctg 301 gaccacttgt cgccatgata ttagctagac ataaagccat ctcTTATTGG TTAGAACTTT 361 TGGGACCAAA TAATAGCTTA GTAGCGAAGG AGACACATCC AGACAGTCTG AGGGCAATTT 421 ATGGCACAGA TGACCTAAGG AATGCACTTC ATGGGAGTAA TGACTTTGCT GCTGCGGAAA 481 GAGAAATACG TTTTATGTTT CCTGAAGTGA TTGTTGAGCC CATTCCAATT GGACAAGCTG 541 CTAAGGACTA TTTAAATTTA CATATAATGC CAACTCTGCT TGAAGGACTC ACAGAGCTTT 601 GTAAGCAAAA ACCAGCAGAT CCTTTGATTT GGCTAGCTGA TTGGCTGCTG AAAAATAATC 661 CTAACAAACC CAAACTTTGT CACCATCCAA TTGTAGAAGA ACCTTATTAG AACCCAGCTT 721 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 781 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 841 CTTGTGGAAA GGACGAACCA GTCTCTATTA CCGGTCTAAT ACGCGTTAAG TCgacaatca 901 acctctggat tacaaaattt gtgaaagatt