Construct: ORF TRCN0000489159
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020467.1_s317c1
- DNA Barcode:
- CACCGTTCCCTTAACTTATACAAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAPK9 (5601)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489159
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5601 | MAPK9 | mitogen-activated protein k... | NM_001364609.2 | 100% | 100% | |
| 2 | human | 5601 | MAPK9 | mitogen-activated protein k... | NM_139070.3 | 100% | 100% | |
| 3 | human | 5601 | MAPK9 | mitogen-activated protein k... | NM_001364608.2 | 97.6% | 97.8% | (many diffs) |
| 4 | human | 5601 | MAPK9 | mitogen-activated protein k... | NM_002752.5 | 97.6% | 97.8% | (many diffs) |
| 5 | human | 5601 | MAPK9 | mitogen-activated protein k... | NM_001364613.2 | 92.3% | 90.3% | (many diffs) |
| 6 | human | 5601 | MAPK9 | mitogen-activated protein k... | NM_001364611.2 | 90% | 88.2% | (many diffs) |
| 7 | human | 5601 | MAPK9 | mitogen-activated protein k... | NM_001364612.1 | 90% | 88.2% | (many diffs) |
| 8 | human | 5601 | MAPK9 | mitogen-activated protein k... | XM_005265940.5 | 90% | 88.2% | (many diffs) |
| 9 | human | 5601 | MAPK9 | mitogen-activated protein k... | NM_139069.3 | 89.3% | 88.9% | 1130_1134delCAGCA;1146_1147ins131 |
| 10 | human | 5601 | MAPK9 | mitogen-activated protein k... | NM_001364607.2 | 87% | 86.7% | (many diffs) |
| 11 | human | 5601 | MAPK9 | mitogen-activated protein k... | NM_139068.3 | 87% | 86.7% | (many diffs) |
| 12 | human | 5601 | MAPK9 | mitogen-activated protein k... | XM_017009642.1 | 86.7% | 86.7% | (many diffs) |
| 13 | human | 5601 | MAPK9 | mitogen-activated protein k... | NM_001135044.2 | 53.7% | 52.8% | (many diffs) |
| 14 | human | 5601 | MAPK9 | mitogen-activated protein k... | NM_001364610.2 | 53.7% | 52.8% | (many diffs) |
| 15 | human | 5601 | MAPK9 | mitogen-activated protein k... | NM_001308244.1 | 49% | 48.1% | (many diffs) |
| 16 | human | 5601 | MAPK9 | mitogen-activated protein k... | XM_017009643.1 | 49% | 48.1% | (many diffs) |
| 17 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008453.2 | 36% | 40.5% | (many diffs) |
| 18 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_017008454.2 | 35.2% | 41.1% | (many diffs) |
| 19 | human | 5602 | MAPK10 | mitogen-activated protein k... | XM_024454149.1 | 35.2% | 41.1% | (many diffs) |
| 20 | mouse | 26420 | Mapk9 | mitogen-activated protein k... | NM_001163671.1 | 91.1% | 98.8% | (many diffs) |
| 21 | mouse | 26420 | Mapk9 | mitogen-activated protein k... | NM_207692.2 | 88.9% | 96.6% | (many diffs) |
| 22 | mouse | 26420 | Mapk9 | mitogen-activated protein k... | XM_017314563.2 | 85.6% | 89.4% | (many diffs) |
| 23 | mouse | 26420 | Mapk9 | mitogen-activated protein k... | XM_017314562.2 | 83.4% | 87.3% | (many diffs) |
| 24 | mouse | 26420 | Mapk9 | mitogen-activated protein k... | NM_016961.3 | 81.5% | 88.2% | (many diffs) |
| 25 | mouse | 26420 | Mapk9 | mitogen-activated protein k... | NM_001163672.1 | 79.4% | 86% | (many diffs) |
| 26 | mouse | 26420 | Mapk9 | mitogen-activated protein k... | XM_030246023.1 | 76% | 78.8% | (many diffs) |
| 27 | mouse | 26420 | Mapk9 | mitogen-activated protein k... | XM_030246022.1 | 73.9% | 76.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1344
- ORF length:
- 1272
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgagcgac agtaaatgtg acagtcagtt ttatagtgtg caagtggcag 121 actcaacctt cactgtccta aaacgttacc agcagctgaa accaattggc tctggggccc 181 aagggattgt ttgtgctgca tttgatacag ttcttgggat aaatgttgca gtcaagaaac 241 taagccgtcc ttttcagaac caaactcatg caaagagagc ttatcgtgaa cttgtcctct 301 taaaatgtgt caatcataaa aatataatta gtttgttaaa tgtgtttaca ccacaaaaaa 361 ctctagaaga atttcaagat gtgtatttgg ttatggaatt aatggatgct aacttatgtc 421 aggttattca catggagctg gatcatgaaa gaatgtccta ccttctttac cagatgcttt 481 gtggtattaa acatctgcat tcagctggta taattcatag agatttgaag cctagcaaca 541 ttgttgtgaa atcagactgc accctgaaga tccttgactt tggcctggcc cggacagcgt 601 gcactaactt catgatgacc ccttacgtgg tgacacggta ctaccgggcg cccgaagtca 661 tcctgggtat gggctacaaa gagaacgtgg acatctggtc tgtcgggtgc atcatggcag 721 aaatggtcct ccataaagtc ctgttcccgg gaagagacta tattgatcag tggaataaag 781 ttattgagca gctgggaaca ccatcagcag agttcatgaa gaaacttcag ccaactgtga 841 ggaattatgt cgaaaacaga ccaaagtatc ctggaatcaa atttgaagaa ctctttccag 901 attggatatt cccatcagaa tctgagcgag acaaaataaa aacaagtcaa gccagagatc 961 tgttatcaaa aatgttagtg attgatccTG ACAAGCGGAT CTCTGTAGAC GAAGCTCTGC 1021 GTCACCCATA CATCACTGTT TGGTATGACC CCGCCGAAGC AGAAGCCCCA CCACCTCAAA 1081 TTTATGATGC CCAGTTGGAA GAAAGAGAAC ATGCAATTGA AGAATGGAAA GAGCTAATTT 1141 ACAAAGAAGT CATGGATTGG GAAGAAAGAA GCAAGAATGG TGTTGTAAAA GATCAGCCTT 1201 CAGATGCAGC AGTAAGTAGC AACGCCACTC CTTCTCAGTC TTCATCGATC AATGACATTT 1261 CATCCATGTC CACTGAGCAG ACGCTGGCCT CAGACACAGA CAGCAGTCTT GATGCCTCGA 1321 CGGGACCCCT TGAAGGCTGT CGATAGGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1381 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1441 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACACCGTTC 1501 CCTTAACTTA TACAAGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1561 aagatt