Construct: ORF TRCN0000489163
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020324.1_s317c1
- DNA Barcode:
- CCTCCTGGGCGGCACTGTCAAACC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PI4K2B (55300)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489163
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55300 | PI4K2B | phosphatidylinositol 4-kina... | NM_018323.4 | 99.7% | 99.7% | 232T>C;321C>T;1173T>C |
| 2 | human | 55300 | PI4K2B | phosphatidylinositol 4-kina... | XM_005248174.2 | 98.7% | 98.7% | (many diffs) |
| 3 | human | 55300 | PI4K2B | phosphatidylinositol 4-kina... | XM_005248175.4 | 79.9% | 80% | 0_1ins288;33C>T;885T>C |
| 4 | human | 55300 | PI4K2B | phosphatidylinositol 4-kina... | NR_144633.2 | 39.9% | (many diffs) | |
| 5 | mouse | 67073 | Pi4k2b | phosphatidylinositol 4-kina... | XM_006504064.2 | 65.8% | 64.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1515
- ORF length:
- 1443
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaggat ccctccgagc ccgaccggtt ggcgtccgcg gacggcggga 121 gcccggagga ggaggaggat ggggagcggg agccgctgct accgcggatc gcctgggccc 181 acccgcggag aggcgcccca ggcagcgccg tgaggctgct ggacgctgcc ggggaggagg 241 gcgaggccgg cgacgaggag ctgcccctcc cgcccgggga cgtgggggtc tcccggagtt 301 cgcccgccga gctggaccgg agccgccccg cggtttcagt aactattggt acttcagaga 361 tgaatgcatt cttggatgac ccagaatttg ctgatattat gctgagagca gagcaagcaa 421 tagaagttgg aatttttcca gaaagaatct ctcaaggttc aagtggaagt tactttgtga 481 aggatcctaa gaggaaaatt attggtgtgt ttaaacccaa atcagaagag ccttatggtc 541 aactcaatcc aaaatggacc aaatatgtcc ataaggtctg ctgcccttgc tgctttggcc 601 gaggctgcct gattcctaat caggggtacc tttccgaagc gggtgcctat cttgtggaca 661 acaagcttca tctgagcatt gtacctaaaa caaaggtggt ttggcttgtc agtgagacat 721 ttaactataa tgcgattgac cgtgcaaaat caagaggcaa aaagtatgct ttagaaaaag 781 tgccaaaagt gggtagaaag tttcatagga taggactccc tcctaagatt ggttcctttc 841 agttatttgt tgaaggttac aaggaggctg aatattggct taggaaattt gaagctgacc 901 ctttgcctga gaatattaga aaacaatttc agtcacaatt tgaaagatta gttattttgg 961 attacatcat cagaaataca gacaggggca atgataattg gttagtcaga tacgaaaagc 1021 agaaatgtga aaaggaaatt gaccataagg aatcaaaatg gattgatgat gaagaattcc 1081 ttattaaaat agctgcaatt gataatggtc tagcatttcc ttttaaacat cctgatgaat 1141 ggagagcata tccatttcac tgggcttggc ttcctcaagc aaaagttccc ttttctgaag 1201 aaataagaaa tttgattcta ccatatattT CTGACATGAA CTTCGTGCAA GATTTATGTG 1261 AAGATCTCTA TGAACTTTTT AAGACTGACA AAGGATTTGA CAAAGCCACT TTTGAAAGTC 1321 AGATGTCTGT GATGAGGGGT CAGATCTTAA ACCTTACTCA GGCATTGAGA GACGGGAAGA 1381 GTCCTTTCCA GCTAGTACAG ATACCTTGTG TGATTGTGGA ACGCAGTCAA GGTGGAAGTC 1441 AGGGTCGGAT TGTCCACCTG AGCAATTCCT TTACCCAGAC TGTCAATTGC AGGAAGCCAT 1501 TTTTTTCCTC CTGGTGAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1561 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1621 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACCTCCTG GGCGGCACTG 1681 TCAAACCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt