Construct: ORF TRCN0000489165
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019237.1_s317c1
- DNA Barcode:
- GGCAGTTCGAACGCACTAAAATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OR51E2 (81285)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489165
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81285 | OR51E2 | olfactory receptor family 5... | NM_030774.4 | 99.7% | 99.6% | 777G>T;960_961insG |
| 2 | mouse | 170639 | Olfr78 | olfactory receptor 78 | NM_001168503.1 | 86.7% | 92.5% | (many diffs) |
| 3 | mouse | 170639 | Olfr78 | olfactory receptor 78 | NM_130866.4 | 86.7% | 92.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1038
- ORF length:
- 963
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgagt tcctgcaact tcacacatgc cacctttgtg cttattggta 121 tcccaggatt agagaaagcc catttctggg ttggcttccc cctcctttcc atgtatgtag 181 tggcaatgtt tggaaactgc atcgtggtct tcatcgtaag gacggaacgc agcctgcacg 241 ctccgatgta cctctttctc tgcatgcttg cagccattga cctggcctta tccacatcca 301 ccatgcctaa gatccttgcc cttttctggt ttgattcccg agagattagc tttgaggcct 361 gtcttaccca gatgttcttt attcatgccc tctcagccat tgaatccacc atcctgctgg 421 ccatggcctt tgaccgttat gtggccatct gccacccact gcgccatgct gcagtgctca 481 acaatacagt aacagcccag attggcatcg tggctgtggt ccgcggatcc ctcttttttt 541 tcccactgcc tctgctgatc aagcggctgg ccttctgcca ctccaatgtc ctctcgcact 601 cctattgtgt ccaccaggat gtaatgaagt tggcctatgc agacactttg cccaatgtgg 661 tatatggTCT TACTGCCATT CTGCTGGTCA TGGGCGTGGA CGTAATGTTC ATCTCCTTGT 721 CCTATTTTCT GATAATACGA ACGGTTCTGC AACTGCCTTC CAAGTCAGAG CGGGCCAAGG 781 CCTTTGGAAC CTGTGTGTCA CACATTGGTG TGGTACTCGC CTTCTATGTG CCACTTATTG 841 GCCTCTCAGT TGTACACCGC TTTGGAAACA GCCTTCATCC CATTGTGCGT GTTGTCATGG 901 GTGACATCTA CCTGCTGCTG CCTCCTGTCA TCAATCCCAT CATCTATGGT GCCAAAACCA 961 AACAGATCAG AACACGGGTG CTGGCTATGT TCAAGATCAG CTGTGACAAG GACTTGCAGG 1021 CTGTGGGAGG CAAGGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1081 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1141 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GGCAGTTCGA ACGCACTAAA 1201 ATTAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt