Construct: ORF TRCN0000489183
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021750.1_s317c1
- DNA Barcode:
- ACCGTTATATTTCTCCACCACGAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DYNC1I1 (1780)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489183
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1780 | DYNC1I1 | dynein cytoplasmic 1 interm... | NM_001135556.1 | 99.8% | 99.8% | 675A>G;997C>G;1440C>A |
| 2 | human | 1780 | DYNC1I1 | dynein cytoplasmic 1 interm... | NM_004411.4 | 97.2% | 97.2% | (many diffs) |
| 3 | human | 1780 | DYNC1I1 | dynein cytoplasmic 1 interm... | NM_001135557.1 | 96.6% | 96.6% | (many diffs) |
| 4 | human | 1780 | DYNC1I1 | dynein cytoplasmic 1 interm... | NM_001278421.1 | 94.1% | 94.1% | (many diffs) |
| 5 | human | 1780 | DYNC1I1 | dynein cytoplasmic 1 interm... | NM_001278422.1 | 91.8% | 89.7% | (many diffs) |
| 6 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | NM_010063.4 | 91.2% | 97.9% | (many diffs) |
| 7 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | NM_001191026.1 | 89.5% | 96.1% | (many diffs) |
| 8 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | XM_017321380.1 | 89.5% | 96.1% | (many diffs) |
| 9 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | XM_006504982.3 | 88.8% | 95.3% | (many diffs) |
| 10 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | NM_001191025.1 | 88.1% | 94.7% | (many diffs) |
| 11 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | NM_001191027.1 | 86.4% | 92.9% | (many diffs) |
| 12 | mouse | 13426 | Dync1i1 | dynein cytoplasmic 1 interm... | NM_001191023.1 | 85.7% | 92.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1953
- ORF length:
- 1884
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gtctgacaaa agtgacttaa aagctgagct agagcgcaaa aagcagcgct 121 tagcacagat aagagaagag aagaaacgga aggaagagga gaggaaaaag aaagaggctg 181 atatgcagca gaagaaagaa cccgttcagg acgactctga tctggatcgc aaacgacgag 241 agacagaggc tttgctgcaa agcattggta tctcaccgga gccgcctcta gtcccaaccc 301 ctatgtctcc ctcctcgaaa tcagtgagca ctcccagtga agctggaagc caagactcag 361 gcgatctggg gccattaaca aggaccctgc agtgggacac agacccctca gtgctccagc 421 tgcagtcaga ctcagaactt ggaagaagac tgcataaact gggcgtgtca aaggtcaccc 481 aagtggattt cctgccaagg gaagtagtgt cctactcaaa ggagacccag actcctcttg 541 ccacgcatca gtctgaagag gatgaggaag atgaggaaat ggtggaatct aaagttggcc 601 aggactcaga actggaaaat caggacaaaa aacaggaagt gaaggaagcc cctccaagag 661 agttgacaga ggaagaaaaa cagcagatcc ttcattcaga ggaatttctc atcttttttg 721 accggacaat acgggtaatt gagagagccc tggctgaaga ttccgacatc ttttttgact 781 acagcggccg agagttagag gaaaaagatg gggatgttca ggctggagcc aatctttctt 841 tcaatcgtca gttctatgat gaacattggt ccaagcatcg agtggtcact tgtatggact 901 ggtccctcca gtaccctgag ctgatggtgg cttcttacaa caacaatgaa gatgctcccc 961 atgaaccaga tggagtggcc ttggtttgga acatgaagtt taagaaaacc acaccagaat 1021 acgtcttcca ctgtcagtcc tctgtgatgt cggtctgctt cgccggtttc catcctaact 1081 tggtggttgg tgggacttac tcgggccaga ttgtcctctg ggacaatcgc agtcatcgaa 1141 ggactccagt gcagcggaca cccttatcag ctgctgcaca cacgcatccc gtgtactgtg 1201 taaatgttgt tgggacccag aatgctcata acctcatcac tgtctccact gatggcaaaa 1261 tgtgttcctg gagcctggac atgctctcaa ctccacagga gagcatggag ctggtgtaca 1321 ataagtccaa gcctgtcgct gttaccggaa tggctttccc aacgggagac gtcaataact 1381 tcgtggttgg cagtgaggaa ggtacagtct acacggcttg tcgtcatgga agcaaagcag 1441 gtattggtga ggtctttgaa ggtcaccaag ggccagtgac aggaattaac tgccacatgg 1501 cagtgggacc aatcgacttt tctcacctgt ttgtcacatc atcatttgac tggactgtca 1561 aactgtggac caccaagcac aacaagccgc tctactcctt tgaagacaat gcagactatg 1621 tgtacgatgt catgtggtcc cccgtgcatc cTGCGCTTTT TGCCTGCGTG GACGGGATGG 1681 GGCGCTTGGA CCTCTGGAAC CTCAACAATG ACACCGAGGT TCCAACAGCA AGTGTGGCCA 1741 TTGAGGGGGC ATCCGCCCTA AACCGTGTTC GTTGGGCCCA AGCTGGCAAA GAAGTTGCTG 1801 TTGGGGACTC GGAAGGCCGT ATTTGGGTCT ATGACGTTGG AGAGCTTGCA GTTCCCCACA 1861 ATGATGAATG GACCCGATTT GCCAGGACCC TTGTGGAAAT TCGTGCTAAC AGAGCTGATA 1921 GCGAGGAGGA AGGCACTGTT GAGTTATCTG CCTAGAACCC AGCTTTCTTG TACAAAGTGG 1981 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2041 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2101 AACCGTTATA TTTCTCCACC ACGAAACGCG TTAAGTCgac aatcaacctc tggattacaa 2161 aatttgtgaa agatt