Construct: ORF TRCN0000489202

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019572.1_s317c1
DNA Barcode:
CGCCCACATCTTCGGCGATACCAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ACSBG1 (23205)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489202
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23205 ACSBG1 acyl-CoA synthetase bubbleg... NM_015162.5 99.9% 99.8% 2172_2173insG
2 human 23205 ACSBG1 acyl-CoA synthetase bubbleg... NM_001199377.2 99.4% 99.3% 446_447insCCTGAAGCTCGG;2160_2161insG
3 human 23205 ACSBG1 acyl-CoA synthetase bubbleg... XM_011521390.3 96% 92.6% (many diffs)
4 human 23205 ACSBG1 acyl-CoA synthetase bubbleg... XM_017022025.2 95.8% 93.8% (many diffs)
5 human 23205 ACSBG1 acyl-CoA synthetase bubbleg... XM_017022026.1 95.5% 92.5% (many diffs)
6 human 23205 ACSBG1 acyl-CoA synthetase bubbleg... XM_011521391.2 86% 83.9% (many diffs)
7 mouse 94180 Acsbg1 acyl-CoA synthetase bubbleg... NM_053178.2 84.4% 86% (many diffs)
8 mouse 94180 Acsbg1 acyl-CoA synthetase bubbleg... XM_006511588.2 82.5% 82.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2241
ORF length:
2175
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaacaat
61tgaccatgcc acgcaattct ggagctggat acggctgccc acacggggac cccagcatgc
121tggacagcag agagacccca caggagagcc ggcaggacat gattgtgagg accacccaag
181aaaaattgaa aaccagctca ctgactgaca ggcagccact ctccaaagag tccctgaacc
241atgctctcga gctctcagtg ccagagaagg tgaataatgc ccagtgggat gctccagagg
301aggcgctgtg gacgactcgg gccgatgggc gggtgcgcct gcgcatagac cccagctgcc
361cacagcttcc ctacactgtg catcggatgt tctacgaggc cctggataag tatggggacc
421tcatcgcttt gggcttcaag cgccaggaca agtgggaaca catctcctac tcccaatact
481acctgctcgc ccgcagagcc gccaagggct tcctgaagct cggcctgaag caggcccaca
541gtgtggccat cctcggcttc aactccccgg agtggttctt ctcggcagtg ggcacagtat
601ttgcaggtgg catcgtcact ggcatctaca ccaccagctc cccagaggcc tgccagtaca
661tcgcttatga ctgctgcgcc aatgtcatca tggtcgacac gcagaagcag ctggaaaaga
721tcctgaagat ctggaaacag ttgccacatc taaaggcagt cgtgatatat aaagaacctc
781ctccaaacaa gatggccaat gtgtacacga tggaggaatt catggagctg gggaatgaag
841tgcctgagga agccctggac gccatcattg acacccagca gcccaaccag tgctgtgtgc
901tagtctacac ttccggcacc actgggaacc ccaagggcgt gatgctgagt caagacaata
961tcacgtggac ggcacggtac ggcagccagg ccggtgacat ccggccggca gaagtccagc
1021aggaggtggt agtcagctac ctgcccctca gccatattgc cgcccagatc tacgacctgt
1081ggacaggcat ccagtggggg gcccaggttt gctttgccga acccgacgcc ctgaagggga
1141gcctggtgaa cacgctgcgg gaggtggagc ccacatcaca catgggggtg ccccgggtat
1201gggagaagat catggagcgc atccaggagg tggcggctca gtctggcttc atccggcgaa
1261agatgctgct gtgggccatg tcggtgacct tggagcagaa cctcacctgc cccggcagcg
1321acctgaagcc cttcacaacc agactggcag attacctggt gctagccaag gttcgccagg
1381cactgggatt tgccaagtgt caaaagaact tctatggagc ggcccccatg atggcagaga
1441cacagcactt cttcctgggt ctcaacatcc gcttgtatgc gggctacggc ctcagtgaga
1501cctcaggccc ccacttcatg tccagtccct acaactaccg gctgtacagc tcaggcaagt
1561tggtgcccgg ctgtcgggtg aagctggtga accaggacgc agagggcatt ggcgagatct
1621gcctgtgggg ccgcaccata ttcatgggct acctgaacat ggaggacaag acttgtgagg
1681ccatcgacga ggaaggctgg ctgcacacgg gtgatgctgg ccgcctggac gccgatggct
1741tcctctacat cactgggcgc ctcaaagaat taatcatcac agctggtggg gagaatgtgc
1801cccctgtgcc catcgaggag gccgtgaaga tggagctgcc catcatcagc aacgccatgc
1861tcattgggga ccagaggaag ttcctgtcca tgctgctcac cttgaagtgc actctggacc
1921cagacaccTC TGACCAGACT GATAATCTGA CTGAACAAGC TATGGAGTTC TGCCAGAGGG
1981TGGGCAGCAG AGCCACCACA GTGTCCGAGA TCATAGAGAA GAAGGATGAG GCCGTGTACC
2041AGGCCATCGA AGAGGGGATC CGGAGGGTCA ACATGAACGC GGCGGCCCGG CCCTACCACA
2101TCCAGAAGTG GGCCATTCTC GAGAGAGACT TCTCCATTTC GGGTGGAGAG TTGGGTCCCA
2161CGATGAAACT GAAACGGCTC ACAGTTTTGG AGAAGTACAA AGGTATCATT GACTCCTTTT
2221ACCAAGAGCA AAAAATGGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
2281TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
2341GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACGCCCAC ATCTTCGGCG
2401ATACCAGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)

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