Construct: ORF TRCN0000489232
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019494.1_s317c1
- DNA Barcode:
- CCATAATTGTCAGCCTTGTTAGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- INSR (3643)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489232
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3643 | INSR | insulin receptor | NM_000208.4 | 99.9% | 99.9% | 5C>G |
| 2 | human | 3643 | INSR | insulin receptor | XM_011527988.2 | 99.9% | 99.7% | 5C>G;2842_2843insTAG |
| 3 | human | 3643 | INSR | insulin receptor | NM_001079817.3 | 99.1% | 99% | 5C>G;2229_2230ins36 |
| 4 | human | 3643 | INSR | insulin receptor | XM_011527989.3 | 99% | 98.9% | 5C>G;2229_2230ins36;2806_2807insTAG |
| 5 | mouse | 16337 | Insr | insulin receptor | NM_001330056.1 | 87% | 95.1% | (many diffs) |
| 6 | mouse | 16337 | Insr | insulin receptor | XM_006508700.1 | 86.9% | 95% | (many diffs) |
| 7 | mouse | 16337 | Insr | insulin receptor | NM_010568.3 | 86.2% | 94.4% | (many diffs) |
| 8 | mouse | 16337 | Insr | insulin receptor | XM_006508701.1 | 86.1% | 94.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 96
- ORF end:
- 4242
- ORF length:
- 4146
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccggt accgctcccg cagccatggg caccgggggc cggcgggggg 121 cggcggccgc gccgctgctg gtggcggtgg ccgcgctgct actgggcgcc gcgggccacc 181 tgtaccccgg agaggtgtgt cccggcatgg atatccggaa caacctcact aggttgcatg 241 agctggagaa ttgctctgtc atcgaaggac acttgcagat actcttgatg ttcaaaacga 301 ggcccgaaga tttccgagac ctcagtttcc ccaaactcat catgatcact gattacttgc 361 tgctcttccg ggtctatggg ctcgagagcc tgaaggacct gttccccaac ctcacggtca 421 tccggggatc acgactgttc tttaactacg cgctggtcat cttcgagatg gttcacctca 481 aggaactcgg cctctacaac ctgatgaaca tcacccgggg ttctgtccgc atcgagaaga 541 acaatgagct ctgttacttg gccactatcg actggtcccg tatcctggat tccgtggagg 601 ataattacat cgtgttgaac aaagatgaca acgaggagtg tggagacatc tgtccgggta 661 ccgcgaaggg caagaccaac tgccccgcca ccgtcatcaa cgggcagttt gtcgaacgat 721 gttggactca tagtcactgc cagaaagttt gcccgaccat ctgtaagtca cacggctgca 781 ccgccgaagg cctctgttgc cacagcgagt gcctgggcaa ctgttctcag cccgacgacc 841 ccaccaagtg cgtggcctgc cgcaacttct acctggacgg caggtgtgtg gagacctgcc 901 cgcccccgta ctaccacttc caggactggc gctgtgtgaa cttcagcttc tgccaggacc 961 tgcaccacaa atgcaagaac tcgcggaggc agggctgcca ccagtacgtc attcacaaca 1021 acaagtgcat ccctgagtgt ccctccgggt acacgatgaa ttccagcaac ttgctgtgca 1081 ccccatgcct gggtccctgt cccaaggtgt gccacctcct agaaggcgag aagaccatcg 1141 actcggtgac gtctgcccag gagctccgag gatgcaccgt catcaacggg agtctgatca 1201 tcaacattcg aggaggcaac aatctggcag ctgagctaga agccaacctc ggcctcattg 1261 aagaaatttc agggtatcta aaaatccgcc gatcctacgc tctggtgtca ctttccttct 1321 tccggaagtt acgtctgatt cgaggagaga ccttggaaat tgggaactac tccttctatg 1381 ccttggacaa ccagaaccta aggcagctct gggactggag caaacacaac ctcaccatca 1441 ctcaggggaa actcttcttc cactataacc ccaaactctg cttgtcagaa atccacaaga 1501 tggaagaagt ttcaggaacc aaggggcgcc aggagagaaa cgacattgcc ctgaagacca 1561 atggggacca ggcatcctgt gaaaatgagt tacttaaatt ttcttacatt cggacatctt 1621 ttgacaagat cttgctgaga tgggagccgt actggccccc cgacttccga gacctcttgg 1681 ggttcatgct gttctacaaa gaggcccctt atcagaatgt gacggagttc gacgggcagg 1741 atgcgtgtgg ttccaacagt tggacggtgg tagacattga cccacccctg aggtccaacg 1801 accccaaatc acagaaccac ccagggtggc tgatgcgggg tctcaagccc tggacccagt 1861 atgccatctt tgtgaagacc ctggtcacct tttcggatga acgccggacc tatggggcca 1921 agagtgacat catttatgtc cagacagatg ccaccaaccc ctctgtgccc ctggatccaa 1981 tctcagtgtc taactcatca tcccagatta ttctgaagtg gaaaccaccc tccgacccca 2041 atggcaacat cacccactac ctggttttct gggagaggca ggcggaagac agtgagctgt 2101 tcgagctgga ttattgcctc aaagggctga agctgccctc gaggacctgg tctccaccat 2161 tcgagtctga agattctcag aagcacaacc agagtgagta tgaggattcg gccggcgaat 2221 gctgctcctg tccaaagaca gactctcaga tcctgaagga gctggaggag tcctcgttta 2281 ggaagacgtt tgaggattac ctgcacaacg tggttttcgt ccccagaaaa acctcttcag 2341 gcactggtgc cgaggaccct aggccatctc ggaaacgcag gtcccttggc gatgttggga 2401 atgtgacggt ggccgtgccc acggtggcag ctttccccaa cacttcctcg accagcgtgc 2461 ccacgagtcc ggaggagcac aggccttttg agaaggtggt gaacaaggag tcgctggtca 2521 tctccggctt gcgacacttc acgggctatc gcatcgagct gcaggcttgc aaccaggaca 2581 cccctgagga acggtgcagt gtggcagcct acgtcagtgc gaggaccatg cctgaagcca 2641 aggctgatga cattgttggc cctgtgacgc atgaaatctt tgagaacaac gtcgtccact 2701 tgatgtggca ggagccgaag gagcccaatg gtctgatcgt gctgtatgaa gtgagttatc 2761 ggcgatatgg tgatgaggag ctgcatctct gcgtctcccg caagcacttc gctctggaac 2821 ggggctgcag gctgcgtggg ctgtcaccgg ggaactacag cgtgcgaatc cgggccacct 2881 cccttgcggg caacggctct tggacggaac ccacctattt ctacgtgaca gactatttag 2941 acgtcccgtc aaatattgca aaaattatca tcggccccct catctttgtc tttctcttca 3001 gtgttgtgat tggaagtatt tatctattcc tgagaaagag gcagccagat gggccgctgg 3061 gaccgcttta cgcttcttca aaccctgagt atctcagtgc cagtgatgtg tttccatgct 3121 ctgtgtacgt gccggacgag tgggaggtgt ctcgagagaa gatcaccctc cttcgagagc 3181 tggggcaggg ctccttcggc atggtgtatg agggcaatgc cagggacatc atcaagggtg 3241 aggcagagac ccgcgtggcg gtgaagacgg tcaacgagtc agccagtctc cgagagcgga 3301 ttgagttcct caatgaggcc tcggtcatga agggcttcac ctgccatcac gtggtgcgcc 3361 tcctgggagt ggtgtccaag ggccagccca cgctggtggt gatggagctg atggctcacg 3421 gagacctgaa gagctacctc cgttctctgc ggccagaggc tgagaataat cctggccgcc 3481 ctccccctac ccttcaagag atgattcaga tggcggcaga gattgctgac gggatggcct 3541 acctgaacgc caagaagttt gtgcatcggg acctggcagc gagaaactgc atggtcgccc 3601 atgattttac tgtcaaaatt ggagactttg gaatgaccag agacatctat gaaacggatt 3661 actaccggaa agggggcaag ggtctgctcc ctgtacggtg gatggcaccg gagtccctga 3721 aggatggggt cttcaccact tcttctgaca tgtggtcctt tggcgtggtc ctttgggaaa 3781 tcaccagctt ggcagaacag ccttaccaag gcctgtctaa tgaacaggtg ttgaaatttg 3841 tcatggatgg agggtatctg gatcaacccg acaactgtcc agagagagtc actgacctca 3901 tgcgcatgtg ctggcaattc aaccccaaga tgaggccaac cttcctggag attgtcaacc 3961 tgctcaagga cgacctgcac cccagctttc cagaggtgtc gttcttccac agcgaggaga 4021 acaaggctcc cgagagtgag gagctggaga tggagtttga ggacatggag aatgtgcccc 4081 tggaccgttc ctcgcactgt cagagggagg aggcgggggg ccgggatgga gggtcctcgc 4141 tgggtttcaa gcggagctac gaggaacaca tcccttacac acacatgaac ggaggcaaga 4201 aaaacgggcG GATTCTGACC TTGCCTCGGT CCAATCCTTC CAAGGGTGGG CGCGCCGACC 4261 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 4321 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 4381 ATATATCTTG TGGAAAGGAC GACCATAATT GTCAGCCTTG TTAGTTACGC GTTAAGTCga 4441 caatcaacct ctggattaca aaatttgtga aagatt