Construct: ORF TRCN0000489236
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021726.1_s317c1
- DNA Barcode:
- TTTGTTTACGTCTACAACCGATTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- EPHA3 (2042)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489236
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2042 | EPHA3 | EPH receptor A3 | XM_005264715.3 | 40.5% | 1_1750del;1819G>A | |
| 2 | human | 2042 | EPHA3 | EPH receptor A3 | NM_005233.6 | 40.5% | 1_1753del;1822G>A | |
| 3 | human | 2042 | EPHA3 | EPH receptor A3 | XM_005264716.3 | 33.8% | (many diffs) | |
| 4 | mouse | 13837 | Epha3 | Eph receptor A3 | NM_010140.3 | 36.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 91
- ORF end:
- 100
- ORF length:
- 9
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctctga aaaaagactt cattttggca atgggcattt aaaacttcca ggtctcagga 121 cttatgttga cccacataca tatgaagacc ctacccaaac tgttcatgag tttgccaagg 181 aattggatgc caccaacata tccattgata aagttgttgg agcaggtgaa tttggagagg 241 tgtgcagtgg tcgcttaaaa cttccttcaa aaaaagagat ttcagtggcc attaagaccc 301 tgaaagttgg ctacacagaa aagcagagga gagacttcct gggagaagca agcattatgg 361 gacagtttga ccaccccaat atcattcgac tggaaggagt tgttaccaaa agtaagccag 421 ttatgattgt cacagaatac atggagaatg gttccttgga tagtttccta cgtaaacacg 481 atgcccagtt tactgtcatt cagctagtgg ggatgcttcg agggatagca tctggcatga 541 agtacctgtc agacatgggc tatgttcacc gagacctcgc tgctcggaac atcttgatca 601 acagtaactt ggtgtgtaag gtttctgatt tcggactttc gcgtgtcctg gaggatgacc 661 cagaagctgc ttatacaaca agaggaggga agatcccaat caggtggaca tcaccagaag 721 ctatagccta ccgcaagttc acgtcagcca gcgatgtatg gagttatggg attgttctct 781 gggaggtgat gtcttatgga gagagaccat actgggagat gtccaatcag gatgtaatta 841 aagctgtaga tgagggctat cgactgccac cccccatgga ctgcccagct gccttgtatc 901 agctgatgct ggactgctgg cagaaagaca ggaacaacag acccaagttt gagcagattG 961 TTAGTATTCT GGACAAGCTT ATCCGGAATC CCGGCAGCCT GAAGATCATC ACCAGTGCAG 1021 CCGCAAGGCC ATCAAACCTT CTTCTGGACC AAAGCAATGT GGATATCACT ACCTTCCGCA 1081 CAACAGGTGA CTGGCTTAAT GGTGTCTGGA CAGCACACTG CAAGGAAATC TTCACGGGTG 1141 TGGAGTACAG TTCTTGTGAC ACAATAGCCA AGATTTCCAC AGATGACATG AAAAAGGTTG 1201 GTGTCACCGT GGTTGGGCCA CAGAAGAAGA TCATCAGTAG CATTAAAGCT CTAGAAACGC 1261 AATCAAAGAA TGGCCCAGTT CCCGTGTAAG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 1321 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1381 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATTTGT 1441 TTACGTCTAC AACCGATTAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1501 tgaaagatt