Construct: ORF TRCN0000489270
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021640.1_s317c1
- DNA Barcode:
- CTGTCCTGCGTCAGACCGGACGCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ESRRB (2103)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489270
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2103 | ESRRB | estrogen related receptor beta | XM_024449508.1 | 95.9% | 95.9% | 1_63del;948T>C |
| 2 | human | 2103 | ESRRB | estrogen related receptor beta | XM_011536553.2 | 93.7% | 93.2% | (many diffs) |
| 3 | human | 2103 | ESRRB | estrogen related receptor beta | NM_004452.3 | 90.3% | 83.5% | (many diffs) |
| 4 | human | 2103 | ESRRB | estrogen related receptor beta | XM_024449509.1 | 90.3% | 83.5% | (many diffs) |
| 5 | human | 2103 | ESRRB | estrogen related receptor beta | XM_011536554.2 | 86.8% | 80.3% | (many diffs) |
| 6 | human | 2103 | ESRRB | estrogen related receptor beta | XM_011536550.2 | 85.9% | 81.4% | (many diffs) |
| 7 | human | 2103 | ESRRB | estrogen related receptor beta | XM_017021085.1 | 85.9% | 81.4% | (many diffs) |
| 8 | human | 2103 | ESRRB | estrogen related receptor beta | XM_011536547.2 | 82.8% | 78.5% | (many diffs) |
| 9 | human | 2103 | ESRRB | estrogen related receptor beta | XR_001750189.1 | 56% | (many diffs) | |
| 10 | human | 2103 | ESRRB | estrogen related receptor beta | XR_943401.2 | 44.5% | 1_533del;1418T>C;2034_3365del | |
| 11 | mouse | 26380 | Esrrb | estrogen related receptor, ... | NM_001159500.1 | 76.2% | 79.6% | (many diffs) |
| 12 | mouse | 26380 | Esrrb | estrogen related receptor, ... | XM_006515881.2 | 75.9% | 79.2% | (many diffs) |
| 13 | mouse | 26380 | Esrrb | estrogen related receptor, ... | NM_011934.4 | 73.8% | 77.1% | (many diffs) |
| 14 | mouse | 26380 | Esrrb | estrogen related receptor, ... | XM_006515882.2 | 56.8% | 52.5% | (many diffs) |
| 15 | mouse | 26380 | Esrrb | estrogen related receptor, ... | XM_006515883.3 | 47.8% | 49.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1572
- ORF length:
- 1500
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtcctcg gacgacaggc acctgggctc cagctgcggc tccttcatca 121 agactgagcc gtccagcccg tcctcgggca tcgatgccct cagccaccac agccccagtg 181 gctcgtccga cgccagcggc ggctttggcc tggccctggg cacccacgcc aacggtctgg 241 actcgccacc catgtttgca ggcgccgggc tgggaggcac cccatgccgc aagagctacg 301 aggactgtgc cagcggcatc atggaggact cggccatcaa gtgcgagtac atgctcaacg 361 ccatccccaa gcgcctgtgc ctcgtgtgcg gggacattgc ctctggctac cactacggcg 421 tggcctcctg cgaggcttgc aaggccttct tcaagaggac tatccaaggg aacattgagt 481 acagctgccc ggccaccaac gagtgcgaga tcaccaaacg gaggcgcaag tcctgccagg 541 cctgccgctt catgaaatgc ctcaaagtgg ggatgctgaa ggaaggtgtg cgccttgatc 601 gagtgcgtgg aggccgtcag aaatacaagc gacggctgga ctcagagagc agcccatacc 661 tgagcttaca aatttctcca cctgctaaaa agccattgac caagattgtc tcatacctac 721 tggtggctga gccggacaag ctctatgcca tgcctccccc tggtatgcct gagggggaca 781 tcaaggccct gaccactctc tgtgacctgg cagaccgaga gcttgtggtc atcattggct 841 gggccaagca catcccaggc ttctcaagcc tctccctggg ggaccagatg agcctgctgc 901 agagtgcctg gatggaaatc ctcatcctgg gcatcgtgta ccgctcgctg ccctacgacg 961 acaagctggt gtacgctgag gactacatca tggatgagga gcactcccgc ctcgcggggc 1021 tgctggagct ctaccgggcc atcctgcagc tggtacgcag gtacaagaag ctcaaggtgg 1081 agaaggagga gtttgtgacg ctcaaggccc tggccctcgc caactccgat tccatgtaca 1141 tcgaggatct agaggctgtc cagaagctgc aggacctGCT GCACGAGGCA CTGCAGGACT 1201 ACGAGCTGAG CCAGCGCCAT GAGGAGCCCT GGAGGACGGG CAAGCTGCTG CTGACACTGC 1261 CGCTGCTGCG GCAGACGGCC GCCAAGGCCG TGCAGCACTT CTATAGCGTC AAACTGCAGG 1321 GCAAAGTGCC CATGCACAAA CTCTTCCTGG AGATGCTGGA GGCCAAGGCC TGGGCCAGGG 1381 CTGACTCCCT TCAGGAGTGG AGGCCACTGG AGCAAGTGCC CTCTCCCCTC CACCGAGCCA 1441 CCAAGAGGCA GCATGTGCAT TTCCTAACTC CCTTGCCCCC TCCCCCATCT GTGGCCTGGG 1501 TGGGCACTGC TCAGGCTGGA TACCACCTGG AGGTTTTCCT TCCGCAGAGG GCAGGTTGGC 1561 CAAGAGCAGC TTGAGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1621 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1681 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTGTCCTGCG TCAGACCGGA 1741 CGCCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt