Construct: ORF TRCN0000489277
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021114.1_s317c1
- DNA Barcode:
- GATAAGCCCCACTCGACTCGCCGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PPAN (56342)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489277
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56342 | PPAN | peter pan homolog | NM_020230.7 | 100% | 100% | |
| 2 | human | 56342 | PPAN | peter pan homolog | NM_001346139.1 | 99.7% | 99.7% | 590_591insTAG |
| 3 | human | 692312 | PPAN-P2RY11 | PPAN-P2RY11 readthrough | NM_001198690.2 | 90% | 86.9% | (many diffs) |
| 4 | human | 56342 | PPAN | peter pan homolog | NM_001346141.1 | 88.7% | 88.7% | 0_1ins159 |
| 5 | human | 692312 | PPAN-P2RY11 | PPAN-P2RY11 readthrough | NM_001040664.3 | 56.9% | 53.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1491
- ORF length:
- 1419
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggacag tcagggaggt cccggcacca gaagcgcgcc cgcgcccagg 121 cgcagctccg caacctcgag gcctatgccg cgaacccgca ctcgttcgtg ttcacgcgag 181 gctgcacggg tcgcaacatc cggcagctca gcctggacgt gcggcgggtc atggagccgc 241 tcactgccag ccgtctgcag gttcgtaaga agaactcgct gaaggactgc gtggcagtgg 301 ctgggcccct cggggtcaca cactttctga tcctgagcaa aacagagacc aatgtctact 361 ttaagctgat gcgcctccca ggaggcccca ccttgacctt ccaggtcaag aagtactcgc 421 tggtgcgtga tgtggtctcc tcactgcgcc ggcaccgcat gcacgagcag cagtttgccc 481 acccacccct cctggtactc aacagctttg gcccccatgg tatgcatgtg aagctcatgg 541 ccaccatgtt ccagaacctg ttcccctcca tcaacgtgca caaggtgaac ctgaacacca 601 tcaagcgctg cctcctcatc gactacaacc ccgactccca ggagctggac ttccgccact 661 atagcatcaa agttgttcct gtgggcgcga gtcgcgggat gaagaagctg ctccaggaga 721 agttccccaa catgagccgc ctgcaggaca tcagcgagct gctggccacg ggcgcggggc 781 tgtcggagag cgaggcagag cctgacggcg accacaacat cacagagctg cctcaggctg 841 tcgctggccg tggcaacatg cgggcccagc agagtgcagt gcggctcacc gagatcggcc 901 cgcggatgac actgcagctc atcaaggtcc aggagggcgt cggggagggc aaagtgatgt 961 tccacagttt tgtgagcaag acggaggagg agctgcaggc catcctggaa gccaaggaga 1021 agaagctgcg gctgaaggcg cagaggcagg cccagcaggc ccagaatgtg cagcgcaagc 1081 aggagcagcg ggaggcccac agaaagaaga gccTGGAGGG CATGAAGAAG GCACGGGTCG 1141 GGGGTAGTGA TGAAGAGGCC TCTGGGATCC CTTCAAGGAC GGCGAGCCTG GAGTTGGGTG 1201 AGGACGATGA TGAACAGGAA GATGATGACA TCGAGTATTT CTGCCAGGCG GTGGGCGAGG 1261 CGCCCAGTGA GGACCTGTTC CCCGAGGCCA AGCAGAAACG GCTTGCCAAG TCTCCAGGGC 1321 GGAAGCGGAA GCGGTGGGAA ATGGATCGAG GCAGGGGTCG CCTTTGTGAC CAGAAGTTTC 1381 CCAAGACCAA GGACAAGTCC CAGGGAGCCC AGGCCAGGCG GGGGCCCAGA GGGGCTTCCC 1441 GGGATGGTGG GCGAGGCCGG GGCCGGGGCC GCCCAGGGAA GAGAGTGGCC TGAGACCCAG 1501 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1561 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1621 TATCTTGTGG AAAGGACGAG ATAAGCCCCA CTCGACTCGC CGGACGCGTT AAGTCgacaa 1681 tcaacctctg gattacaaaa tttgtgaaag att