Transcript: Human NM_020230.7

Homo sapiens peter pan homolog (PPAN), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-10
Taxon:
Homo sapiens (human)
Gene:
PPAN (56342)
Length:
2302
CDS:
34..1455

Additional Resources:

NCBI RefSeq record:
NM_020230.7
NBCI Gene record:
PPAN (56342)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_020230.7, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000414370 AGCTGGACTTCCGCCACTATA pLKO_005 605 CDS 100% 13.200 6.600 Y PPAN n/a
2 TRCN0000256389 GAGACCAATGTCTACTTTAAG pLKO_005 307 CDS 100% 13.200 6.600 Y PPAN-P2RY11 n/a
3 TRCN0000256474 GATGATGACATCGAGTATTTC pLKO_005 1183 CDS 100% 13.200 6.600 Y PPAN-P2RY11 n/a
4 TRCN0000256390 TCCGCCACTATAGCATCAAAG pLKO_005 614 CDS 100% 10.800 5.400 Y PPAN-P2RY11 n/a
5 TRCN0000256475 CCGTCTGCAGGTTCGTAAGAA pLKO_005 213 CDS 100% 5.625 2.813 Y PPAN-P2RY11 n/a
6 TRCN0000129631 CAAAGTGATGTTCCACAGTTT pLKO.1 912 CDS 100% 4.950 2.475 Y PPAN n/a
7 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 2139 3UTR 100% 4.950 2.475 Y ERAP2 n/a
8 TRCN0000128301 GAAGATGATGACATCGAGTAT pLKO.1 1180 CDS 100% 4.950 2.475 Y PPAN n/a
9 TRCN0000422640 GTCGCCTTTGTGACCAGAAGT pLKO_005 1319 CDS 100% 4.950 2.475 Y PPAN n/a
10 TRCN0000424356 TCGTAAGAAGAACTCGCTGAA pLKO_005 225 CDS 100% 4.050 2.025 Y PPAN n/a
11 TRCN0000127527 CAAGGTGAACCTGAACACCAT pLKO.1 543 CDS 100% 2.640 1.320 Y PPAN n/a
12 TRCN0000128039 GAGGACGATGATGAACAGGAA pLKO.1 1162 CDS 100% 2.640 1.320 Y PPAN n/a
13 TRCN0000127764 GATGACACTGCAGCTCATCAA pLKO.1 867 CDS 100% 0.495 0.248 Y PPAN n/a
14 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 2140 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_020230.7, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03720 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_03720 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000469669 GACGATCAAGCCGATCGCTGGTAC pLX_317 26.9% 100% 100% V5 n/a
4 TRCN0000491355 GGTGGCATAAGATCCCCTACGTCA pLX_317 21.5% 100% 100% V5 n/a
5 TRCN0000489277 GATAAGCCCCACTCGACTCGCCGG pLX_317 27% 100% 100% V5 (not translated due to prior stop codon) n/a
6 ccsbBroadEn_08639 pDONR223 100% 99.9% 100% None 1125G>A n/a
7 ccsbBroad304_08639 pLX_304 0% 99.9% 100% V5 1125G>A n/a
8 TRCN0000479902 TCAGTCCCAGTGGCACTCCAAGTC pLX_317 23.6% 99.9% 100% V5 1125G>A n/a
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