Transcript: Human NM_001198690.2

Homo sapiens PPAN-P2RY11 readthrough (PPAN-P2RY11), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-06-02
Taxon:
Homo sapiens (human)
Gene:
PPAN-P2RY11 (692312)
Length:
3114
CDS:
34..1596

Additional Resources:

NCBI RefSeq record:
NM_001198690.2
NBCI Gene record:
PPAN-P2RY11 (692312)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001198690.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000414370 AGCTGGACTTCCGCCACTATA pLKO_005 605 CDS 100% 13.200 6.600 Y PPAN n/a
2 TRCN0000256389 GAGACCAATGTCTACTTTAAG pLKO_005 307 CDS 100% 13.200 6.600 Y PPAN-P2RY11 n/a
3 TRCN0000009470 GAGAGAGTGGAGCTGCTTTAT pLKO.1 2758 3UTR 100% 13.200 6.600 Y P2RY11 n/a
4 TRCN0000256474 GATGATGACATCGAGTATTTC pLKO_005 1183 CDS 100% 13.200 6.600 Y PPAN-P2RY11 n/a
5 TRCN0000256390 TCCGCCACTATAGCATCAAAG pLKO_005 614 CDS 100% 10.800 5.400 Y PPAN-P2RY11 n/a
6 TRCN0000256475 CCGTCTGCAGGTTCGTAAGAA pLKO_005 213 CDS 100% 5.625 2.813 Y PPAN-P2RY11 n/a
7 TRCN0000358024 ACAGGACTGGAGACGCAAGAA pLKO_005 2721 3UTR 100% 4.950 2.475 Y P2RY11 n/a
8 TRCN0000367850 AGACACCCAAGTGGCATCTTG pLKO_005 2926 3UTR 100% 4.950 2.475 Y P2RY11 n/a
9 TRCN0000358025 AGGGTGGCAGGTATAAGACTT pLKO_005 2884 3UTR 100% 4.950 2.475 Y P2RY11 n/a
10 TRCN0000129631 CAAAGTGATGTTCCACAGTTT pLKO.1 912 CDS 100% 4.950 2.475 Y PPAN n/a
11 TRCN0000357957 CGTGAGCTGAGCCAATGATGT pLKO_005 2463 3UTR 100% 4.950 2.475 Y P2RY11 n/a
12 TRCN0000128301 GAAGATGATGACATCGAGTAT pLKO.1 1180 CDS 100% 4.950 2.475 Y PPAN n/a
13 TRCN0000256473 GCCCATACTGGTGGTTGAGTT pLKO_005 1451 CDS 100% 4.950 2.475 Y PPAN-P2RY11 n/a
14 TRCN0000422640 GTCGCCTTTGTGACCAGAAGT pLKO_005 1319 CDS 100% 4.950 2.475 Y PPAN n/a
15 TRCN0000009472 TGCCCGAGCTTTGCAGACATA pLKO.1 2199 3UTR 100% 4.950 2.475 Y P2RY11 n/a
16 TRCN0000424356 TCGTAAGAAGAACTCGCTGAA pLKO_005 225 CDS 100% 4.050 2.025 Y PPAN n/a
17 TRCN0000127527 CAAGGTGAACCTGAACACCAT pLKO.1 543 CDS 100% 2.640 1.320 Y PPAN n/a
18 TRCN0000009473 CGACGACAAACTCAGTGGGTT pLKO.1 1412 CDS 100% 2.640 1.320 Y P2RY11 n/a
19 TRCN0000128039 GAGGACGATGATGAACAGGAA pLKO.1 1162 CDS 100% 2.640 1.320 Y PPAN n/a
20 TRCN0000009471 GCGCTTCCTCTTCACCTGCAA pLKO.1 1670 3UTR 100% 0.880 0.440 Y P2RY11 n/a
21 TRCN0000127764 GATGACACTGCAGCTCATCAA pLKO.1 867 CDS 100% 0.495 0.248 Y PPAN n/a
22 TRCN0000009474 CCTCTACTCTACATGGCCGCA pLKO.1 2307 3UTR 100% 0.180 0.090 Y P2RY11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001198690.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03720 pDONR223 100% 90% 86.9% None (many diffs) n/a
2 ccsbBroad304_03720 pLX_304 0% 90% 86.9% V5 (many diffs) n/a
3 TRCN0000469669 GACGATCAAGCCGATCGCTGGTAC pLX_317 26.9% 90% 86.9% V5 (many diffs) n/a
4 TRCN0000491355 GGTGGCATAAGATCCCCTACGTCA pLX_317 21.5% 90% 86.9% V5 (many diffs) n/a
5 TRCN0000489277 GATAAGCCCCACTCGACTCGCCGG pLX_317 27% 90% 86.9% V5 (not translated due to prior stop codon) (many diffs) n/a
6 ccsbBroadEn_08639 pDONR223 100% 89.9% 86.9% None (many diffs) n/a
7 ccsbBroad304_08639 pLX_304 0% 89.9% 86.9% V5 (many diffs) n/a
8 TRCN0000479902 TCAGTCCCAGTGGCACTCCAAGTC pLX_317 23.6% 89.9% 86.9% V5 (many diffs) n/a
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