Construct: ORF TRCN0000489279
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021067.1_s317c1
- DNA Barcode:
- AAATCCCACGTAACTACTAATCCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GRM5 (2915)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489279
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2915 | GRM5 | glutamate metabotropic rece... | NM_000842.4 | 99.9% | 100% | 3279A>C |
| 2 | human | 2915 | GRM5 | glutamate metabotropic rece... | XM_017017627.2 | 99.9% | 100% | 3279A>C |
| 3 | human | 2915 | GRM5 | glutamate metabotropic rece... | NM_001143831.2 | 97.3% | 97.3% | 2629_2724del;3375A>C |
| 4 | human | 2915 | GRM5 | glutamate metabotropic rece... | XM_006718828.4 | 97.3% | 97.3% | 2629_2724del;3375A>C |
| 5 | human | 2915 | GRM5 | glutamate metabotropic rece... | XM_011542792.1 | 97.3% | 97.3% | 2629_2724del;3375A>C |
| 6 | mouse | 108071 | Grm5 | glutamate receptor, metabot... | NM_001081414.2 | 88.6% | 95.3% | (many diffs) |
| 7 | mouse | 108071 | Grm5 | glutamate receptor, metabot... | NM_001143834.1 | 86.3% | 92.8% | (many diffs) |
| 8 | mouse | 108071 | Grm5 | glutamate receptor, metabot... | XM_011241643.2 | 86.3% | 92.8% | (many diffs) |
| 9 | mouse | 108071 | Grm5 | glutamate receptor, metabot... | XM_006507184.3 | 74.3% | 75.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3612
- ORF length:
- 3540
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggtcctt ctgttgatcc tgtcagtctt acttttgaaa gaagatgtcc 121 gtgggagtgc acagtccagt gagaggaggg tggtggctca catgccgggt gacatcatta 181 ttggagctct cttttctgtt catcaccagc ctactgtgga caaagttcat gagaggaagt 241 gtggggcggt ccgtgaacag tatggcattc agagagtgga ggccatgctg cataccctgg 301 aaaggatcaa ttcagacccc acactcttgc ccaacatcac actgggctgt gagataaggg 361 actcctgctg gcattcggct gtggccctag agcagagcat tgagttcata agagattccc 421 tcatttcttc agaagaggaa gaaggcttgg tacgctgtgt ggatggctcc tcctcttcct 481 tccgctccaa gaagcccata gtaggggtca ttgggcctgg ctccagttct gtagccattc 541 aggtccagaa tttgctccag cttttcaaca tacctcagat tgcttactca gcaaccagca 601 tggatctgag tgacaagact ctgttcaaat atttcatgag ggttgtgcct tcagatgctc 661 agcaggcaag ggccatggtg gacatagtga agaggtacaa ctggacctat gtatcagccg 721 tgcacacaga aggcaactat ggagaaagtg ggatggaagc cttcaaagat atgtcagcga 781 aggaagggat ttgcatcgcc cactcttaca aaatctacag taatgcaggg gagcagagct 841 ttgataagct gctgaagaag ctcacaagtc acttgcccaa ggcccgggtg gtggcctgct 901 tctgtgaggg catgacggtg agaggtctgc tgatggccat gaggcgcctg ggtctagcgg 961 gagaatttct gcttctgggc agtgatggct gggctgacag gtatgatgtg acagatggat 1021 atcagcgaga agctgttggt ggcatcacaa tcaagctcca atctcccgat gtcaagtggt 1081 ttgatgatta ttatctgaag ctccggccag aaacaaacca ccgaaaccct tggtttcaag 1141 aattttggca gcatcgtttt cagtgccgac tggaagggtt tccacaggag aacagcaaat 1201 acaacaagac ttgcaatagt tctctgactc tgaaaacaca tcatgttcag gattccaaaa 1261 tgggatttgt gatcaacgcc atctattcga tggcctatgg gctccacaac atgcagatgt 1321 ccctctgccc aggctatgca ggactctgtg atgccatgaa gccaattgat ggacggaaac 1381 ttttggagtc cctgatgaaa accaatttta ctggggtttc tggagatacg atcctattcg 1441 atgagaatgg agactctcca ggaaggtatg aaataatgaa tttcaaggaa atgggaaaag 1501 attactttga ttatatcaac gttggaagtt gggacaatgg agaattaaaa atggatgatg 1561 atgaagtatg gtccaagaaa agcaacatca tcagatctgt gtgcagtgaa ccatgtgaga 1621 aaggccagat caaggtgatc cgaaagggag aagtcagctg ttgttggacc tgtacacctt 1681 gtaaggagaa tgagtatgtc tttgatgagt acacatgcaa ggcatgccaa ctggggtctt 1741 ggcccactga tgatctcaca ggttgtgact tgatcccagt acagtatctt cgatggggtg 1801 accctgaacc cattgcagct gtggtgtttg cctgccttgg cctcctggcc accctgtttg 1861 ttactgtagt cttcatcatt taccgtgata caccagtagt caagtcctca agcagggaac 1921 tctgctacat tatccttgct ggcatctgcc tgggctactt atgtaccttc tgcctcattg 1981 cgaagcccaa acagatttac tgctaccttc agagaattgg cattggtctc tccccagcca 2041 tgagctactc agcccttgta acaaagacca accgtattgc aaggatcctg gctggcagca 2101 agaagaagat ctgtaccaaa aagcccagat tcatgagtgc ctgtgcccag ctagtgattg 2161 ctttcattct catatgcatc cagttgggca tcatcgttgc cctctttata atggagcctc 2221 ctgacataat gcatgactac ccaagcattc gagaagtcta cctgatctgt aacaccacca 2281 acctaggagt tgtcactcca cttggataca atggattgtt gattttgagc tgcaccttct 2341 atgcgttcaa gaccagaaat gttccagcta acttcaacga ggccaagtat atcgccttca 2401 caatgtacac gacctgcatt atatggctag cttttgtgcc aatctacttt ggcagcaact 2461 acaaaatcat caccatgtgt ttctcggtca gcctcagtgc cacagtggcc ctaggctgca 2521 tgtttgtgcc gaaggtgtac atcatcctgg ccaaaccaga gagaaacgtg cgcagcgcct 2581 tcaccacatc taccgtggtg cgcatgcatg taggggatgg caagtcatcc tccgcagcca 2641 gcagatccag cagcctagtc aacctgtgga agagaagggg ctcctctggg gaaaccttaa 2701 gttccaatgg aaaatccgtc acgtgggccc agaatgagaa gagcagccgg gggcagcacc 2761 tgtggcagcg cctgtccatc cacatcaaca agaaagaaaa ccccaaccaa acggccgtca 2821 tcaagccctt ccccaagagc acggagagcc gtggcctggg cgctggcgct ggcgcaggcg 2881 ggagcgctgg gggcgtgggg gccacgggcg gtgcgggctg cgcaggcgcc ggcccaggcg 2941 ggcccgagtc cccagacgcc ggccccaagg cgctgtatga tgtggccgag gctgaggagc 3001 acttcccggc gcccgcgcgg ccgcgctcac cgtcgcccat cagcacgctg agccaccgcg 3061 cgggctcggc cagccgcacg gacgacgatg tgccgtcgct gcactcggag cctgtggcgc 3121 gcagcagctc ctcgcagggc tccctcatgg agcagatcag cagtgtggtc acccgcttca 3181 cggccaacat cagcgagctc aactccatga tgctgtccac cgcggccccc agccccggcg 3241 tcggcgcccc gctctgctcg tcctacctGA TCCCCAAAGA GATCCAGTTG CCCACGACCA 3301 TGACGACCTT TGCCGAAATC CAGCCTCTGC CGGCCATCGA AGTCACGGGC GGCGCGCAGC 3361 CCGCGGCAGG GGCGCAGGCG GCTGGGGACG CGGCCCGGGA GAGCCCCGCG GCCGGTCCCG 3421 AGGCTGCGGC CGCCAAGCCA GACCTGGAGG AGCTGGTGGC TCTCACCCCG CCGTCCCCCT 3481 TCAGAGACTC GGTGGACTCG GGGAGCACAA CCCCCAACTC GCCAGTGTCC GAGTCGGCCC 3541 TCTGTATCCC GTCGTCTCCC AAATATGACA CTCTTATCAT AAGAGATTAC ACTCAGAGCT 3601 CCTCGTCGTT GTGAGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 3661 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 3721 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AAATCCCACG TAACTACTAA 3781 TCCAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt