Construct: ORF TRCN0000489279

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021067.1_s317c1
DNA Barcode:
AAATCCCACGTAACTACTAATCCA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
GRM5 (2915)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489279
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2915 GRM5 glutamate metabotropic rece... NM_000842.4 99.9% 100% 3279A>C
2 human 2915 GRM5 glutamate metabotropic rece... XM_017017627.2 99.9% 100% 3279A>C
3 human 2915 GRM5 glutamate metabotropic rece... NM_001143831.2 97.3% 97.3% 2629_2724del;3375A>C
4 human 2915 GRM5 glutamate metabotropic rece... XM_006718828.4 97.3% 97.3% 2629_2724del;3375A>C
5 human 2915 GRM5 glutamate metabotropic rece... XM_011542792.1 97.3% 97.3% 2629_2724del;3375A>C
6 mouse 108071 Grm5 glutamate receptor, metabot... NM_001081414.2 88.6% 95.3% (many diffs)
7 mouse 108071 Grm5 glutamate receptor, metabot... NM_001143834.1 86.3% 92.8% (many diffs)
8 mouse 108071 Grm5 glutamate receptor, metabot... XM_011241643.2 86.3% 92.8% (many diffs)
9 mouse 108071 Grm5 glutamate receptor, metabot... XM_006507184.3 74.3% 75.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
3612
ORF length:
3540
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggtcctt ctgttgatcc tgtcagtctt acttttgaaa gaagatgtcc
121gtgggagtgc acagtccagt gagaggaggg tggtggctca catgccgggt gacatcatta
181ttggagctct cttttctgtt catcaccagc ctactgtgga caaagttcat gagaggaagt
241gtggggcggt ccgtgaacag tatggcattc agagagtgga ggccatgctg cataccctgg
301aaaggatcaa ttcagacccc acactcttgc ccaacatcac actgggctgt gagataaggg
361actcctgctg gcattcggct gtggccctag agcagagcat tgagttcata agagattccc
421tcatttcttc agaagaggaa gaaggcttgg tacgctgtgt ggatggctcc tcctcttcct
481tccgctccaa gaagcccata gtaggggtca ttgggcctgg ctccagttct gtagccattc
541aggtccagaa tttgctccag cttttcaaca tacctcagat tgcttactca gcaaccagca
601tggatctgag tgacaagact ctgttcaaat atttcatgag ggttgtgcct tcagatgctc
661agcaggcaag ggccatggtg gacatagtga agaggtacaa ctggacctat gtatcagccg
721tgcacacaga aggcaactat ggagaaagtg ggatggaagc cttcaaagat atgtcagcga
781aggaagggat ttgcatcgcc cactcttaca aaatctacag taatgcaggg gagcagagct
841ttgataagct gctgaagaag ctcacaagtc acttgcccaa ggcccgggtg gtggcctgct
901tctgtgaggg catgacggtg agaggtctgc tgatggccat gaggcgcctg ggtctagcgg
961gagaatttct gcttctgggc agtgatggct gggctgacag gtatgatgtg acagatggat
1021atcagcgaga agctgttggt ggcatcacaa tcaagctcca atctcccgat gtcaagtggt
1081ttgatgatta ttatctgaag ctccggccag aaacaaacca ccgaaaccct tggtttcaag
1141aattttggca gcatcgtttt cagtgccgac tggaagggtt tccacaggag aacagcaaat
1201acaacaagac ttgcaatagt tctctgactc tgaaaacaca tcatgttcag gattccaaaa
1261tgggatttgt gatcaacgcc atctattcga tggcctatgg gctccacaac atgcagatgt
1321ccctctgccc aggctatgca ggactctgtg atgccatgaa gccaattgat ggacggaaac
1381ttttggagtc cctgatgaaa accaatttta ctggggtttc tggagatacg atcctattcg
1441atgagaatgg agactctcca ggaaggtatg aaataatgaa tttcaaggaa atgggaaaag
1501attactttga ttatatcaac gttggaagtt gggacaatgg agaattaaaa atggatgatg
1561atgaagtatg gtccaagaaa agcaacatca tcagatctgt gtgcagtgaa ccatgtgaga
1621aaggccagat caaggtgatc cgaaagggag aagtcagctg ttgttggacc tgtacacctt
1681gtaaggagaa tgagtatgtc tttgatgagt acacatgcaa ggcatgccaa ctggggtctt
1741ggcccactga tgatctcaca ggttgtgact tgatcccagt acagtatctt cgatggggtg
1801accctgaacc cattgcagct gtggtgtttg cctgccttgg cctcctggcc accctgtttg
1861ttactgtagt cttcatcatt taccgtgata caccagtagt caagtcctca agcagggaac
1921tctgctacat tatccttgct ggcatctgcc tgggctactt atgtaccttc tgcctcattg
1981cgaagcccaa acagatttac tgctaccttc agagaattgg cattggtctc tccccagcca
2041tgagctactc agcccttgta acaaagacca accgtattgc aaggatcctg gctggcagca
2101agaagaagat ctgtaccaaa aagcccagat tcatgagtgc ctgtgcccag ctagtgattg
2161ctttcattct catatgcatc cagttgggca tcatcgttgc cctctttata atggagcctc
2221ctgacataat gcatgactac ccaagcattc gagaagtcta cctgatctgt aacaccacca
2281acctaggagt tgtcactcca cttggataca atggattgtt gattttgagc tgcaccttct
2341atgcgttcaa gaccagaaat gttccagcta acttcaacga ggccaagtat atcgccttca
2401caatgtacac gacctgcatt atatggctag cttttgtgcc aatctacttt ggcagcaact
2461acaaaatcat caccatgtgt ttctcggtca gcctcagtgc cacagtggcc ctaggctgca
2521tgtttgtgcc gaaggtgtac atcatcctgg ccaaaccaga gagaaacgtg cgcagcgcct
2581tcaccacatc taccgtggtg cgcatgcatg taggggatgg caagtcatcc tccgcagcca
2641gcagatccag cagcctagtc aacctgtgga agagaagggg ctcctctggg gaaaccttaa
2701gttccaatgg aaaatccgtc acgtgggccc agaatgagaa gagcagccgg gggcagcacc
2761tgtggcagcg cctgtccatc cacatcaaca agaaagaaaa ccccaaccaa acggccgtca
2821tcaagccctt ccccaagagc acggagagcc gtggcctggg cgctggcgct ggcgcaggcg
2881ggagcgctgg gggcgtgggg gccacgggcg gtgcgggctg cgcaggcgcc ggcccaggcg
2941ggcccgagtc cccagacgcc ggccccaagg cgctgtatga tgtggccgag gctgaggagc
3001acttcccggc gcccgcgcgg ccgcgctcac cgtcgcccat cagcacgctg agccaccgcg
3061cgggctcggc cagccgcacg gacgacgatg tgccgtcgct gcactcggag cctgtggcgc
3121gcagcagctc ctcgcagggc tccctcatgg agcagatcag cagtgtggtc acccgcttca
3181cggccaacat cagcgagctc aactccatga tgctgtccac cgcggccccc agccccggcg
3241tcggcgcccc gctctgctcg tcctacctGA TCCCCAAAGA GATCCAGTTG CCCACGACCA
3301TGACGACCTT TGCCGAAATC CAGCCTCTGC CGGCCATCGA AGTCACGGGC GGCGCGCAGC
3361CCGCGGCAGG GGCGCAGGCG GCTGGGGACG CGGCCCGGGA GAGCCCCGCG GCCGGTCCCG
3421AGGCTGCGGC CGCCAAGCCA GACCTGGAGG AGCTGGTGGC TCTCACCCCG CCGTCCCCCT
3481TCAGAGACTC GGTGGACTCG GGGAGCACAA CCCCCAACTC GCCAGTGTCC GAGTCGGCCC
3541TCTGTATCCC GTCGTCTCCC AAATATGACA CTCTTATCAT AAGAGATTAC ACTCAGAGCT
3601CCTCGTCGTT GTGAGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
3661CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
3721TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AAATCCCACG TAACTACTAA
3781TCCAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)

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