Construct: ORF TRCN0000489284

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020523.1_s317c1
DNA Barcode:
GCGGTGAGATTTCGAGTGCTTGCG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
WNK1 (65125)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489284
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 65125 WNK1 WNK lysine deficient protei... XM_017019839.1 19% 18.8% (many diffs)
2 human 65125 WNK1 WNK lysine deficient protei... XM_011521009.2 18.5% 18.2% (many diffs)
3 human 65125 WNK1 WNK lysine deficient protei... XM_011521008.2 18.5% 18.2% (many diffs)
4 human 65125 WNK1 WNK lysine deficient protei... NM_014823.3 18.2% 18% (many diffs)
5 human 65125 WNK1 WNK lysine deficient protei... XM_017019838.1 18.2% 18% (many diffs)
6 human 65125 WNK1 WNK lysine deficient protei... XM_017019837.1 18.2% 18% (many diffs)
7 human 65125 WNK1 WNK lysine deficient protei... XM_017019836.1 17.7% 17.4% (many diffs)
8 human 65125 WNK1 WNK lysine deficient protei... XM_017019835.1 17.7% 17.4% (many diffs)
9 human 65125 WNK1 WNK lysine deficient protei... XM_017019834.1 17.5% 17.2% (many diffs)
10 human 65125 WNK1 WNK lysine deficient protei... XM_011521007.2 16.5% 16.3% (many diffs)
11 human 65125 WNK1 WNK lysine deficient protei... XM_011521006.2 16.5% 16.3% (many diffs)
12 human 65125 WNK1 WNK lysine deficient protei... XM_006719003.2 16.3% 16.1% (many diffs)
13 human 65125 WNK1 WNK lysine deficient protei... NM_018979.4 16.3% 16.1% (many diffs)
14 human 65125 WNK1 WNK lysine deficient protei... XM_011521004.2 15.3% 15.1% (many diffs)
15 human 65125 WNK1 WNK lysine deficient protei... XM_011521003.2 15.3% 15% (many diffs)
16 human 65125 WNK1 WNK lysine deficient protei... NM_213655.4 14.8% 14.6% (many diffs)
17 human 65125 WNK1 WNK lysine deficient protei... XM_011521002.2 14.7% 14.5% (many diffs)
18 human 65125 WNK1 WNK lysine deficient protei... NM_001184985.1 14.7% 14.5% (many diffs)
19 human 65125 WNK1 WNK lysine deficient protei... XM_011521001.2 14.4% 14.2% (many diffs)
20 human 65125 WNK1 WNK lysine deficient protei... XM_011521000.2 14.2% 14% (many diffs)
21 human 65125 WNK1 WNK lysine deficient protei... XM_011520998.2 13.9% 13.7% (many diffs)
22 human 65125 WNK1 WNK lysine deficient protei... XM_011520999.2 13.9% 13.7% (many diffs)
23 human 65125 WNK1 WNK lysine deficient protei... XM_011520997.3 13.9% 13.7% (many diffs)
24 mouse 232341 Wnk1 WNK lysine deficient protei... NM_001185020.1 15.8% 16.7% (many diffs)
25 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321557.1 15.8% 16.7% (many diffs)
26 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321556.1 15.4% 16.3% (many diffs)
27 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321555.1 15.4% 16.2% (many diffs)
28 mouse 232341 Wnk1 WNK lysine deficient protei... NM_001185021.1 15.3% 16.1% (many diffs)
29 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321554.1 15.3% 16.1% (many diffs)
30 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321553.1 15.1% 15.9% (many diffs)
31 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321552.1 14.8% 15.6% (many diffs)
32 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321550.1 14.5% 15.3% (many diffs)
33 mouse 232341 Wnk1 WNK lysine deficient protei... NM_198703.3 14.1% 14.9% (many diffs)
34 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321549.1 14.1% 14.9% (many diffs)
35 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321548.1 14.1% 14.8% (many diffs)
36 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321547.1 14% 14.8% (many diffs)
37 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321546.1 14% 14.7% (many diffs)
38 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321545.1 13.9% 14.7% (many diffs)
39 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321544.1 13.9% 14.6% (many diffs)
40 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321543.1 13.9% 14.6% (many diffs)
41 mouse 232341 Wnk1 WNK lysine deficient protei... XM_011241320.2 13.8% 14.6% (many diffs)
42 mouse 232341 Wnk1 WNK lysine deficient protei... XM_017321542.1 13.8% 14.6% (many diffs)
43 mouse 232341 Wnk1 WNK lysine deficient protei... NM_001199083.1 12.8% 13.5% (many diffs)
44 mouse 232341 Wnk1 WNK lysine deficient protei... NM_001199084.1 12.8% 13.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1251
ORF length:
1179
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgtctggc ggcgccgcag agaagcagag cagcactccc ggttccctgt
121tcctctcgcc gccggctcct gcccccaaga atggctccag ctccgattcc tccgtggggg
181agaaactggg agccgcggcc gccgacgctg tgaccggcag gaccgaggag tacaggcgcc
241gccgccacac tatggacaag gacagccgtg gggcggccgc gaccactacc accactgagc
301accgcttctt ccgccggagc gtcatctgcg actccaatgc cactgcactg gagcttcccg
361gccttcctct ttccctgccc cagcccagca tccccgcggc tgtcccgcag agtgctccac
421cggagcccca ccgggaagag accgtgaccg ccaccgccac ttcccaggta gcccagcagc
481ctccagccgc tgccgcccct ggggaacagg ccgtcgcggg ccctgccccc tcgactgtcc
541ccagcagtac cagcaaagac cgcccagtgt cccagcctag ccttgtgggg agcaaagagg
601agccgccgcc ggcgagaagt ggcagcggcg gcggcagcgc caaggagcca caggaggaac
661ggagccagca gcaggatgat atcgaagagc tggagaccaa ggccgtggga atgtctaacg
721atggccgctt tctcaagttt gacatcgaaa tcggcagagg ctcctttaag acggtctaca
781aaggtctgga cactgaaacc accgtggaag tcgcctggtg tgaactgcag gatcgaaaat
841taacaaagtc TGAGAGGCAG AGATTTAAAG AAGAAGCTGA AATGTTAAAA GGTCTTCAGC
901ATCCCAATAT TGTTAGATTT TATGATTCCT GGGAATCCAC AGTAAAAGGA AAGAAGTGCA
961TTGTTTTGGT GACTGAACTT ATGACGTCTG GAACACTTAA AACGTATCTG AAAAGGTTTA
1021AAGTGATGAA GATCAAAGTT CTAAGAAGCT GGTGCCGTCA GATCCTTAAA GGTCTTCAGT
1081TTCTTCATAC TCGAACTCCA CCTATCATTC ACCGCGATCT TAAATGTGAC AACATCTTTA
1141TCACCGGCCC TACTGGCTCA GTCAAGATTG GAGACCTCGG TCTGGCAACC CTGAAGCGGG
1201CTTCTTTTGC CAAGAGTGTG ATAGGTATGT TTCAGGTGTA CCTTGGAGCC TAGAACCCAG
1261CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
1321ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
1381TATCTTGTGG AAAGGACGAG CGGTGAGATT TCGAGTGCTT GCGACGCGTT AAGTCgacaa
1441tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)

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