Construct: ORF TRCN0000489285
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020420.1_s317c1
- DNA Barcode:
- CCATGCAAAGCCTCTTAGCTCCGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- LIMK2 (3985)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489285
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3985 | LIMK2 | LIM domain kinase 2 | NM_005569.4 | 99.9% | 100% | 1209C>G |
| 2 | human | 3985 | LIMK2 | LIM domain kinase 2 | NM_016733.3 | 95.3% | 94.5% | (many diffs) |
| 3 | human | 3985 | LIMK2 | LIM domain kinase 2 | NM_001031801.2 | 83.9% | 74.3% | (many diffs) |
| 4 | mouse | 16886 | Limk2 | LIM motif-containing protei... | NM_010718.4 | 88.2% | 93.1% | (many diffs) |
| 5 | mouse | 16886 | Limk2 | LIM motif-containing protei... | XM_006514548.2 | 87.4% | 92.2% | (many diffs) |
| 6 | mouse | 16886 | Limk2 | LIM motif-containing protei... | NM_173053.1 | 85.6% | 89.9% | (many diffs) |
| 7 | mouse | 16886 | Limk2 | LIM motif-containing protei... | XM_006514550.3 | 84.8% | 89.1% | (many diffs) |
| 8 | mouse | 16886 | Limk2 | LIM motif-containing protei... | XM_006514547.1 | 80.5% | 84.9% | (many diffs) |
| 9 | mouse | 16886 | Limk2 | LIM motif-containing protei... | XM_011243666.2 | 77.3% | 82.7% | (many diffs) |
| 10 | mouse | 16886 | Limk2 | LIM motif-containing protei... | NM_001034030.2 | 62.9% | 67.2% | (many diffs) |
| 11 | mouse | 16886 | Limk2 | LIM motif-containing protei... | XM_006514552.3 | 62.3% | 66.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 84
- ORF end:
- 1998
- ORF length:
- 1914
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggcccccttc accatgtccg cgctggcggg tgaagatgtc tggaggtgtc 121 caggctgtgg ggaccacatt gctccaagcc agatatggta caggactgtc aacgaaacct 181 ggcacggctc ttgcttccgg tgttcagaat gccaggattc cctcaccaac tggtactatg 241 agaaggatgg gaagctctac tgccccaagg actactgggg gaagtttggg gagttctgtc 301 atgggtgctc cctgctgatg acagggcctt ttatggtggc tggggagttc aagtaccacc 361 cagagtgctt tgcctgtatg agctgcaagg tgatcattga ggatggggat gcatatgcac 421 tggtgcagca tgccaccctc tactgtggga agtgccacaa tgaggtggtg ctggcaccca 481 tgtttgagag actctccaca gagtctgttc aggagcagct gccctactct gtcacgctca 541 tctccatgcc ggccaccact gaaggcaggc ggggcttctc cgtgtccgtg gagagtgcct 601 gctccaacta cgccaccact gtgcaagtga aagaggtcaa ccggatgcac atcagtccca 661 acaatcgaaa cgccatccac cctggggacc gcatcctgga gatcaatggg acccccgtcc 721 gcacacttcg agtggaggag gtggaggatg caattagcca gacgagccag acacttcagc 781 tgttgattga acatgacccc gtctcccaac gcctggacca gctgcggctg gaggcccggc 841 tcgctcctca catgcagaat gccggacacc cccacgccct cagcaccctg gacaccaagg 901 agaatctgga ggggacactg aggagacgtt ccctaaggcg cagtaacagt atctccaagt 961 cccctggccc cagctcccca aaggagcccc tgctgttcag ccgtgacatc agccgctcag 1021 aatcccttcg ttgttccagc agctattcac agcagatctt ccggccctgt gacctaatcc 1081 atggggaggt cctggggaag ggcttctttg ggcaggctat caaggtgaca cacaaagcca 1141 cgggcaaagt gatggtcatg aaagagttaa ttcgatgtga tgaggagacc cagaaaactt 1201 ttctgactga ggtgaaagtg atgcgcagcc tggaccaccc caatgtgctc aagttcattg 1261 gtgtgctgta caaggataag aagctgaacc tgctgacaga gtacattgag gggggcacac 1321 tgaaggactt tctgcgcagt atggatccgt tcccctggca gcagaaggtc aggtttgcca 1381 aaggaatcgc ctccggaatg gcctatttgc actctatgtg catcatccac cgggatctga 1441 actcgcacaa ctgcctcatc aagttggaca agactgtggt ggtggcagac tttgggctgt 1501 cacggctcat agtggaagag aggaaaaggg cccccatgga gaaggccacc accaagaaac 1561 gcaccttgcg caagaacgac cgcaagaagc gctacacggt ggtgggaaac ccctactgga 1621 tggcccctGA GATGCTGAAC GGAAAGAGCT ATGATGAGAC GGTGGATATC TTCTCCTTTG 1681 GGATCGTTCT CTGTGAGATC ATTGGGCAGG TGTATGCAGA TCCTGACTGC CTTCCCCGAA 1741 CACTGGACTT TGGCCTCAAC GTGAAGCTTT TCTGGGAGAA GTTTGTTCCC ACAGATTGTC 1801 CCCCGGCCTT CTTCCCGCTG GCCGCCATCT GCTGCAGACT GGAGCCTGAG AGCAGACCAG 1861 CATTCTCGAA ATTGGAGGAC TCCTTTGAGG CCCTCTCCCT GTACCTGGGG GAGCTGGGCA 1921 TCCCGCTGCC TGCAGAGCTG GAGGAGTTGG ACCACACTGT GAGCATGCAG TACGGCCTGA 1981 CCCGGGACTC ACCTCCCTAG TCTAGAAAGG GTGGGCGCGC CGACCCAGCT TTCTTGTACA 2041 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2101 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2161 AGGACGACCA TGCAAAGCCT CTTAGCTCCG GACGCGTTAA GTCgacaatc aacctctgga 2221 ttacaaaatt tgtgaaagat t