Construct: ORF TRCN0000489301
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019302.1_s317c1
- DNA Barcode:
- CAGTTGCCCTAATCGCATATATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- QRFPR (84109)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489301
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84109 | QRFPR | pyroglutamylated RFamide pe... | NM_198179.3 | 99.9% | 100% | 816C>T |
| 2 | human | 84109 | QRFPR | pyroglutamylated RFamide pe... | XM_017008693.2 | 43.6% | 43.3% | (many diffs) |
| 3 | mouse | 229214 | Qrfpr | pyroglutamylated RFamide pe... | NM_198192.2 | 83.8% | 83.1% | (many diffs) |
| 4 | mouse | 243407 | C130060K24Rik | RIKEN cDNA C130060K24 gene | NM_175524.4 | 81.4% | 78.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 60
- ORF end:
- 1353
- ORF length:
- 1293
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaacca 61 tgcaggcgct taacattacc ccggagcagt tctctcggct gctgcgggac cacaacctga 121 cgcgggagca gttcatcgct ctgtaccggc tgcgaccgct cgtctacacc ccagagctgc 181 cgggacgcgc caagctggcc ctcgtgctca ccggcgtgct catcttcgcc ctggcgctct 241 ttggcaatgc tctggtgttc tacgtggtga cccgcagcaa ggccatgcgc accgtcacca 301 acatctttat ctgctccttg gcgctcagtg acctgctcat caccttcttc tgcattcccg 361 tcaccatgct ccagaacatt tccgacaact ggctgggggg tgctttcatt tgcaagatgg 421 tgccatttgt ccagtctacc gctgttgtga cagaaatcct cactatgacc tgcattgctg 481 tggaaaggca ccagggactt gtgcatcctt ttaaaatgaa gtggcaatac accaaccgaa 541 gggctttcac aatgctaggt gtggtctggc tggtggcagt catcgtagga tcacccatgt 601 ggcacgtgca acaacttgag atcaaatatg acttcctata tgaaaaggaa cacatctgct 661 gcttagaaga gtggaccagc cctgtgcacc agaagatcta caccaccttc atccttgtca 721 tcctcttcct cctgcctctt atggtgatgc ttattctgta cagtaaaatt ggttatgaac 781 tttggataaa gaaaagagtt ggggatggtt cagtgcttcg aactattcat ggaaaagaaa 841 tgtccaaaat agccaggaag aagaaacgag ctgttattat gatggtgaca gtggtggctc 901 tctttgctgt gtgctgggca ccattccatg ttgtccatat gatgattgaa tacagtaatt 961 ttgaaaagga atatgatgat gtcacaatca agatgatttt tgctatcgtg caaattattg 1021 gattttccaa ctccatctgt aatcccattG TCTATGCATT TATGAATGAA AACTTCAAAA 1081 AAAATGTTTT GTCTGCAGTT TGTTATTGCA TAGTAAATAA AACCTTCTCT CCAGCACAAA 1141 GGCATGGAAA TTCAGGAATT ACAATGATGC GGAAGAAAGC AAAGTTTTCC CTCAGAGAGA 1201 ATCCAGTGGA GGAAACCAAA GGAGAAGCAT TCAGTGATGG CAACATTGAA GTCAAATTGT 1261 GTGAACAGAC AGAGGAGAAG AAAAAGCTCA AACGACATCT TGCTCTCTTT AGGTCTGAAC 1321 TGGCTGAGAA TTCTCCTTTA GACAGTGGGC ATGACCCAGC TTTCTTGTAC AAAGTGGTTG 1381 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1441 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA 1501 GTTGCCCTAA TCGCATATAT CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1561 ttgtgaaaga tt