Construct: ORF TRCN0000489305
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021775.1_s317c1
- DNA Barcode:
- CAAAAAAAAAAACCAAACAGTCCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MGLL (11343)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489305
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11343 | MGLL | monoglyceride lipase | NM_007283.6 | 100% | 100% | |
| 2 | human | 11343 | MGLL | monoglyceride lipase | NM_001003794.2 | 96.8% | 96.8% | 0_1ins30 |
| 3 | human | 11343 | MGLL | monoglyceride lipase | XM_011512377.2 | 92.3% | 92.3% | 510_587del |
| 4 | human | 11343 | MGLL | monoglyceride lipase | NM_001256585.1 | 90.4% | 90.4% | 508_509ins90 |
| 5 | human | 11343 | MGLL | monoglyceride lipase | XM_011512378.2 | 89.3% | 89.3% | 0_1ins30;480_557del |
| 6 | human | 11343 | MGLL | monoglyceride lipase | XM_017005666.2 | 87.2% | 87.2% | 0_1ins30;478_479ins90 |
| 7 | human | 11343 | MGLL | monoglyceride lipase | XM_017005662.2 | 86.9% | 79.7% | (many diffs) |
| 8 | human | 11343 | MGLL | monoglyceride lipase | XM_017005664.2 | 79.5% | 75.3% | (many diffs) |
| 9 | human | 11343 | MGLL | monoglyceride lipase | XM_017005665.1 | 79.5% | 75.3% | (many diffs) |
| 10 | human | 11343 | MGLL | monoglyceride lipase | XM_024453333.1 | 79.5% | 75.3% | (many diffs) |
| 11 | human | 11343 | MGLL | monoglyceride lipase | XM_024453334.1 | 76.6% | 76.6% | 0_1ins219 |
| 12 | human | 11343 | MGLL | monoglyceride lipase | XM_011512379.2 | 73.6% | 69.6% | (many diffs) |
| 13 | human | 11343 | MGLL | monoglyceride lipase | XM_011512382.1 | 70.7% | 70.7% | 0_1ins219;291_368del |
| 14 | human | 11343 | MGLL | monoglyceride lipase | XM_017005663.1 | 70.7% | 60% | (many diffs) |
| 15 | human | 11343 | MGLL | monoglyceride lipase | XM_011512383.2 | 70.2% | 65.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1008
- ORF length:
- 939
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggaaacagga cctgaagacc cttccagcat gccagaggaa agttccccca 121 ggcggacccc gcagagcatt ccctaccagg acctccctca cctggtcaat gcagacggac 181 agtacctctt ctgcaggtac tggaaaccca caggcacacc caaggccctc atctttgtgt 241 cccatggagc cggagagcac agtggccgct atgaagagct ggctcggatg ctgatggggc 301 tggacctgct ggtgttcgcc cacgaccatg ttggccacgg acagagcgaa ggggagagga 361 tggtagtgtc tgacttccac gttttcgtca gggatgtgtt gcagcatgtg gattccatgc 421 agaaagacta ccctgggctt cctgtcttcc ttctgggcca ctccatggga ggcgccatcg 481 ccatcctcac ggccgcagag aggccgggcc acttcgccgg catggtactc atttcgcctc 541 tggttcttgc caatcctgaa tctgcaacaa ctttcaaggt ccttgctgcg aaagtgctca 601 accttgtgct gccaaacttg tccctcgggc ccatcgactc cagcgtgctc tctcggaata 661 agacagaggt cgacatttat aactcagacc cccTGATCTG CCGGGCAGGG CTGAAGGTGT 721 GCTTCGGCAT CCAACTGCTG AATGCCGTCT CACGGGTGGA GCGCGCCCTC CCCAAGCTGA 781 CTGTGCCCTT CCTGCTGCTC CAGGGCTCTG CCGATCGCCT ATGTGACAGC AAAGGGGCCT 841 ACCTGCTCAT GGAGTTAGCC AAGAGCCAGG ACAAGACTCT CAAGATTTAT GAAGGTGCCT 901 ACCATGTTCT CCACAAGGAG CTTCCTGAAG TCACCAACTC CGTCTTCCAT GAAATAAACA 961 TGTGGGTCTC TCAAAGGACA GCCACGGCAG GAACTGCGTC CCCACCCTAG AACCCAGCTT 1021 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1081 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1141 CTTGTGGAAA GGACGACAAA AAAAAAAACC AAACAGTCCG ACGCGTTAAG TCgacaatca 1201 acctctggat tacaaaattt gtgaaagatt