Construct: ORF TRCN0000489314
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021243.1_s317c1
- DNA Barcode:
- CTCAAACTTACCCCAGACTTGTGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- EBAG9 (9166)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489314
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9166 | EBAG9 | estrogen receptor binding s... | NM_001278938.1 | 100% | 100% | |
| 2 | human | 9166 | EBAG9 | estrogen receptor binding s... | NM_004215.5 | 100% | 100% | |
| 3 | human | 9166 | EBAG9 | estrogen receptor binding s... | NM_198120.2 | 100% | 100% | |
| 4 | human | 9166 | EBAG9 | estrogen receptor binding s... | XM_017013960.1 | 100% | 100% | |
| 5 | mouse | 55960 | Ebag9 | estrogen receptor-binding f... | NM_019480.4 | 91% | 97.6% | (many diffs) |
| 6 | mouse | 55960 | Ebag9 | estrogen receptor-binding f... | XM_006521193.2 | 91% | 97.6% | (many diffs) |
| 7 | mouse | 55960 | Ebag9 | estrogen receptor-binding f... | XM_006521194.3 | 91% | 97.6% | (many diffs) |
| 8 | mouse | 55960 | Ebag9 | estrogen receptor-binding f... | XM_006521195.3 | 91% | 97.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 711
- ORF length:
- 639
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccatc acccagtttc ggttatttaa attttgtacc tgcctagcaa 121 cagtattctc attcctaaag agattaatat gcagatctgg cagaggacgg aaattaagtg 181 gagaccaaat aactttgcca actacagttg attattcatc agttcctaag cagacagatg 241 ttgaagagtg gacttcctgg gatgaagatg cacccaccag tgtaaagatc gaaggaggga 301 atgggaatgt ggcaacacaa caaaattctt tGGAACAACT GGAACCTGAC TATTTTAAGG 361 ACATGACACC AACTATTAGG AAAACTCAGA AAATTGTTAT TAAGAAGAGA GAACCATTGA 421 ATTTTGGCAT CCCAGATGGG AGCACAGGTT TCTCTAGTAG ATTAGCAGCT ACACAAGATC 481 TGCCTTTTAT TCATCAGTCT TCTGAATTAG GTGACTTAGA TACCTGGCAG GAAAATACCA 541 ATGCATGGGA AGAAGAAGAA GATGCAGCCT GGCAAGCAGA AGAAGTTCTG AGACAGCAGA 601 AACTAGCAGA CAGAGAAAAG AGAGCAGCCG AACAACAAAG GAAGAAAATG GAAAAGGAAG 661 CACAACGGCT AATGAAGAAG GAACAAAACA AAATTGGTGT GAAACTTTCA TAGAACCCAG 721 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 781 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 841 TATCTTGTGG AAAGGACGAC TCAAACTTAC CCCAGACTTG TGTACGCGTT AAGTCgacaa 901 tcaacctctg gattacaaaa tttgtgaaag att