Construct: ORF TRCN0000489318
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021084.1_s317c1
- DNA Barcode:
- GCTCGAGGCGATTACGCTCATCTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADGRB1 (575)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489318
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 575 | ADGRB1 | adhesion G protein-coupled ... | NM_001702.3 | 89.8% | 87.8% | (many diffs) |
| 2 | human | 575 | ADGRB1 | adhesion G protein-coupled ... | XM_017013696.1 | 87.9% | 86% | (many diffs) |
| 3 | human | 575 | ADGRB1 | adhesion G protein-coupled ... | XM_017013695.1 | 87.6% | 85.7% | (many diffs) |
| 4 | human | 575 | ADGRB1 | adhesion G protein-coupled ... | XM_017013694.1 | 87.3% | 85.4% | (many diffs) |
| 5 | human | 575 | ADGRB1 | adhesion G protein-coupled ... | XM_017013692.1 | 85.6% | 83.7% | (many diffs) |
| 6 | human | 575 | ADGRB1 | adhesion G protein-coupled ... | XM_017013691.1 | 85.5% | 83.7% | (many diffs) |
| 7 | human | 575 | ADGRB1 | adhesion G protein-coupled ... | XM_017013693.1 | 85.4% | 83.5% | (many diffs) |
| 8 | human | 575 | ADGRB1 | adhesion G protein-coupled ... | XM_017013697.1 | 82.2% | 80.4% | (many diffs) |
| 9 | human | 575 | ADGRB1 | adhesion G protein-coupled ... | XM_011517202.2 | 81.5% | 79.7% | 3117G>C;3397_3398ins100;3483_3484ins689 |
| 10 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | NM_174991.3 | 79.5% | 83.6% | (many diffs) |
| 11 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | XM_011245404.1 | 79.2% | 83.3% | (many diffs) |
| 12 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | XM_011245403.1 | 77.9% | 81.9% | (many diffs) |
| 13 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | XM_006520269.2 | 77.6% | 81.7% | (many diffs) |
| 14 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | XM_006520270.2 | 77.6% | 81.6% | (many diffs) |
| 15 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | XM_006520271.2 | 77.4% | 81.5% | (many diffs) |
| 16 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | XM_011245406.2 | 70.6% | 75.4% | (many diffs) |
| 17 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | XM_011245405.1 | 69.9% | 73.5% | (many diffs) |
| 18 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | XR_383967.3 | 65.5% | (many diffs) | |
| 19 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | XR_383969.2 | 59.3% | (many diffs) | |
| 20 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | XR_875215.1 | 59.1% | (many diffs) | |
| 21 | mouse | 107831 | Adgrb1 | adhesion G protein-coupled ... | XM_006520276.2 | 44.2% | 45.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 4344
- ORF length:
- 4272
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaggggc caggccgccg ccccgggccc cgtctggatc ctcgccccgc 121 tgctactgct gctgctgctg ctgggacgcc gcgcgcgggc ggccgccgga gcagacgcgg 181 ggcccgggcc cgagccgtgc gccacgctgg tgcagggaaa gttcttcggc tacttctccg 241 cggccgccgt gttcccggcc aacgcctcgc gctgctcctg gacgctacgc aacccggacc 301 cgcggcgcta cactctctac atgaaggtgg ccaaggcgcc cgtgccctgc agcggccccg 361 gccgcgtgcg cacctaccag ttcgactcct tcctcgagtc cacgcgcacc tacctgggcg 421 tggagagctt cgacgaggtg ctgcggctct gcgacccctc cgcacccctg gccttcctgc 481 aggccagcaa gcagttcctg cagatgcggc gccagcagcc gccccagcac gacgggctcc 541 ggccccgggc cgggccgccg ggccccaccg acgacttctc cgtggagtac ctggtggtgg 601 ggaaccgcaa ccccagccgt gccgcctgcc agatgctgtg ccgctggctg gacgcgtgtc 661 tggccggtag tcgcagctcg cacccctgcg ggatcatgca gaccccctgc gcctgcctgg 721 gcggcgaggc gggcggccct gccgcgggac ccctggcccc ccgcggggat gtctgcttga 781 gagatgcggt ggctggtggc cctgaaaact gcctcaccag cctgacccag gaccggggcg 841 ggcacggcgc cacaggcggc tggaagctgt ggtccctgtg gggcgaatgc acgcgggact 901 gcgggggagg cctccagacg cggacgcgca cctgcctgcc cgcgccgggc gtggagggcg 961 gcggctgcga gggggtgctg gaggagggtc gccagtgcaa ccgcgaggcc tgcggccccg 1021 ctgggcgcac cagctcccgg agccagtccc tgcggtccac agatgcccgg cggcgcgagg 1081 agctggggga cgagctgcag cagtttgggt tcccagcccc ccagaccggt gacccagcag 1141 ccgaggagtg gtccccgtgg agcgtgtgct ccagcacctg cggcgagggc tggcagaccc 1201 gcacgcgctt ctgcgtgtcc tcctcctaca gcacgcagtg cagcggaccc ctgcgcgagc 1261 agcggctgtg caacaactct gccgtgtgcc cagtgcatgg tgcctgggat gagtggtcgc 1321 cctggagcct ctgctccagc acctgtggcc gtggctttcg ggatcgcacg cgcacctgca 1381 ggccccccca gtttgggggc aacccctgtg agggccctga gaagcaaacc aagttctgca 1441 acattgccct gtgccctggc cgggcagtgg atggaaactg gaatgagtgg tcgagctgga 1501 gcgcctgctc cgccagctgc tcccagggcc gacagcagcg cacgcgtgaa tgcaacgggc 1561 cttcctacgg gggtgcggag tgccagggcc actgggtgga gacccgagac tgcttcctgc 1621 agcagtgccc agtggatggc aagtggcagg cctgggcgtc atggggcagt tgcagcgtca 1681 cgtgtggggc tggcagccag cgacgggagc gtgtctgctc tgggcccttc ttcgggggag 1741 cagcctgcca gggcccccag gatgagtacc ggcagtgcgg cacccagcgg tgtcccgagc 1801 cccatgagat ctgtgatgag gacaactttg gtgctgtgat ctggaaggag accccagcgg 1861 gagaggtggc tgctgtccgg tgtccccgca acgccacagg actcatcctg cgacggtgtg 1921 agctggacga ggaaggcatc gcctactggg agccccccac ctacatccgc tgtgtttcca 1981 ttgactacag aaacatccag atgatgaccc gggagcacct ggccaaggct cagcgagggc 2041 tgcctgggga gggggtctcg gaggtcatcc agacactggt ggagatctct caggacggga 2101 ccagctacag tggggacctg ctgtccacca tcgatgtcct gaggaacatg acagagattt 2161 tccggagagc gtactacagc cccacccctg gggacgtaca gaactttgtc cagatcctta 2221 gcaacctgtt ggcagaggag aatcgggaca agtgggagga ggcccagctg gcgggcccca 2281 acgccaagga gctgttccgg ctggtggagg actttgtgga cgtcatcggc ttccgcatga 2341 aggacctgag ggatgcatac caggtgacag acaacctggt tctcagcatc cataagctcc 2401 cagccagcgg agccactgac atcagcttcc ccatgaaggg ctggcgggcc acgggtgact 2461 gggccaaggt gccagaggac agggtcactg tgtccaagag tgtcttctcc acggggctga 2521 cagaggccga tgaagcatcc gtgtttgtgg tgggcaccgt gctctacagg aacctgggca 2581 gcttcctggc cctgcagagg aacacgaccg tcctgaattc taaggtgatc tccgtgactg 2641 tgaaaccccc gcctcgctcc ctgcgcacac ccttggagat cgagtttgcc cacatgtata 2701 atggcaccac caaccagacc tgtatcctgt gggatgagac ggatgtaccc tcctcctccg 2761 cccccccgca gctcgggccc tggtcgtggc gcggctgccg cacggtgccc ctcgacgccc 2821 tccggacgcg ctgcctctgt gaccggctct ccaccttcgc catcttagcc cagctcagcg 2881 ccgacgcgaa catggagaag gcgactctgc cgtcggtgac gctcatcgtg ggctgtggcg 2941 tgtcctctct caccctgctc atgctggtca tcatctacgt gtccgtgtgg aggtacattc 3001 gctcagagcg ttctgtcatc ctcatcaact tctgcctgtc catcatctcc tccaatgccc 3061 tcatcctcat cgggcagacc cagacccgca acaaggtggt gtgcacgctg gtggccgcct 3121 tcctgcactt cttcttcctg tcctccttct gctgggtgct caccgaggcc tggcagtcct 3181 acatggccgt gacgggccac ctccggaacc gcctcatccg caagcgcttc ctctgcctgg 3241 gctgggggct ccctgcactg gttgtggcca tttctgtggg attcaccaag gccaaagggt 3301 acagcaccat gaactactgc tggctctccc tggagggggg actgctctat gccttcgtgg 3361 gacctgccgc tgccgttgtg ctggtgaaca tggtcattgg gatcctggtg ttcaacaagc 3421 tcgtgtccaa agacggcatc acggacaaga agctgaagga gcgggcaggg gcctccctgt 3481 ggagctcctg cgtggtgctg ccgctgctgg cgctgacctg gatgtcggct gtgctcgccg 3541 tcaccgaccg ccgctccgcc ctcttccaga tcctcttcgc tgtcttcgac tcgctggagg 3601 gcttcgtcat cgtcatggtg cactgtatcc tccgtagaga ggtccaggac gctgtgaaat 3661 gccgtgtggt tgaccggcag gaggagggca acggggactc agggggctcc ttccagaacg 3721 gccacgccca gctcatgacc gacttcgaga aggacgtgga tctggcctgt agatcagtgc 3781 tgaacaagga catcgcggcc tgccgcactg ccaccatcac gggcacactg aagcggccgt 3841 ctctgcccga ggaggagaag ctgaagctgg cccatgccaa ggggccgccc accaatttca 3901 acagcctgcc ggccaacgtg tccaagctgc acctgcacgg ctcaccccgc tatcccggcg 3961 ggcccctgcc cgacttcccc aaccactcac tgaccctcaa gagggacaag gcgcccaagt 4021 cctccTTCGT CGGTGACGGG GACATCTTCA AGAAGCTGGA CTCGGATCTG AGCCGGGCCC 4081 AGGAGAAGGC TCTGGACACG AGCTACGTGA TCCTGCCCAC GGCCACGGCC ACGCTGCGGC 4141 CCAAGCCCAA GGAGGAGCCC AAGTACAGCA TCCACATTGA CCAGATGCCG CAGACCCGCC 4201 TCATCCACCT CAGCAGCCCC TGCCCCCACC GCCCAATCTG GAGCCGGCAC CCCCCAGCCT 4261 GGGGGATCCC GGGGAGCCTG CCGCCCATCC GGGACCCAGC ACGGGGCCCA GCACCAAGAA 4321 CGAGAATGTC GCCACCTTGT CTGTGAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 4381 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 4441 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCTCGAGG 4501 CGATTACGCT CATCTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 4561 aagatt