Construct: ORF TRCN0000489322
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020668.1_s317c1
- DNA Barcode:
- TTTTATGGAACCTATCATTCAATT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NEK3 (4752)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489322
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4752 | NEK3 | NIMA related kinase 3 | NM_002498.3 | 100% | 100% | |
| 2 | human | 4752 | NEK3 | NIMA related kinase 3 | NM_152720.2 | 100% | 100% | |
| 3 | human | 4752 | NEK3 | NIMA related kinase 3 | NM_001146099.1 | 96.6% | 96.6% | 876_877ins51 |
| 4 | human | 4752 | NEK3 | NIMA related kinase 3 | NR_164641.1 | 68.9% | 1_112del;989_990ins50;1581_2078del | |
| 5 | human | 4752 | NEK3 | NIMA related kinase 3 | NR_027415.2 | 62.8% | 1_376del;1245_1246insA;1894_2412del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1590
- ORF length:
- 1518
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggatgac tacatggtcc tgagaatgat tggggagggc tccttcggca 121 gagctctttt ggttcagcat gaaagcagta atcagatgtt tgccatgaaa gaaataaggc 181 ttcccaagtc tttctctaat acacagaatt ctaggaagga ggctgttctt ttagccaaaa 241 tgaaacaccc taatattgtt gccttcaaag aatcatttga agctgaagga cacttgtata 301 ttgtgatgga atactgtgat ggaggggatc taatgcaaaa gattaaacag cagaaaggaa 361 agttatttcc tgaagacatg atacttaatt ggtttaccca aatgtgcctt ggagtaaatc 421 acattcacaa gaaacgtgtg ctacacagag atatcaagtc caagaatatc ttcctcactc 481 agaatggaaa agtgaaattg ggagactttg gatctgcccg tcttctctcc aatccgatgg 541 catttgcttg tacctatgtg ggaactcctt attatgtgcc tccagaaatt tgggaaaacc 601 tgccttataa caataaaagt gacatctggt ccttgggttg catcctgtat gaactctgta 661 cccttaagca tccatttcag gcaaatagtt ggaaaaatct tatcctcaaa gtatgtcaag 721 ggtgcatcag tccactgccg tctcattact cctatgaact tcagttccta gtcaagcaga 781 tgtttaaaag gaatccctca catcgcccct cggctacaac gcttctctct cgaggcatcg 841 tagctcggct tgtccagaag tgcttacccc ccgagatcat catggaatat ggtgaggaag 901 tattagaaga aataaaaaat tcgaagcata acacaccaag aaaaaaaaca aaccccagca 961 gaatcaggat agctttggga aatgaagcaa gcacagtgca agaggaagaa caagatagaa 1021 agggtagcca tactgatttg gaaagcatta atgaaaattt agttgaaagt gcattgagaa 1081 gagtaaacag agaagaaaaa ggtaataagt cagtccatct gaggaaagcc agttcaccaa 1141 atcttcatag acgacagtgg gagaaaaatg tacccaatac agctcttaca gctttggaaa 1201 atgcatccat actcaccTCC AGTTTAACAG CAGAGGACGA TAGAGGTGGT TCTGTAATAA 1261 AGTACAGCAA AAATACTACT CGTAAGCAGT GGCTCAAAGA GACCCCTGAC ACTTTGTTGA 1321 ACATCCTTAA GAATGCTGAT CTCAGCTTGG CTTTTCAAAC ATACACAATA TATAGACCAG 1381 GTTCAGAAGG GTTCTTGAAA GGCCCCCTGT CTGAAGAAAC AGAAGCATCG GACAGTGTTG 1441 ATGGAGGTCA CGATTCTGTC ATTTTGGATC CAGAGCGACT TGAGCCTGGG CTAGATGAGG 1501 AGGACACGGA CTTTGAGGAG GAAGATGACA ACCCCGACTG GGTGTCAGAG CTGAAGAAGC 1561 GAGCTGGATG GCAAGGCCTG TGCGACAGAT AGAACCCAGC TTTCTTGTAC AAAGTGGTTG 1621 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1681 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT 1741 TTATGGAACC TATCATTCAA TTACGCGTTA AGTCgacaat caacctctgg attacaaaat 1801 ttgtgaaaga tt