Construct: ORF TRCN0000489350
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021722.1_s317c1
- DNA Barcode:
- CGCGGCAGTCTCCAGTCATTGATT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- NTRK2 (4915)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489350
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4915 | NTRK2 | neurotrophic receptor tyros... | NM_001369535.1 | 52.2% | 1% | 1_949del;1994_1998delTAGGC |
| 2 | human | 4915 | NTRK2 | neurotrophic receptor tyros... | NM_001369536.1 | 51% | 1% | 1_997del;2042_2046delTAGGC |
| 3 | human | 4915 | NTRK2 | neurotrophic receptor tyros... | NM_001369534.1 | 42.9% | .8% | 1_1381del;2426_2430delTAGGC |
| 4 | human | 4915 | NTRK2 | neurotrophic receptor tyros... | NM_001018064.3 | 42.3% | .8% | 1_1417del;2462_2466delTAGGC |
| 5 | human | 4915 | NTRK2 | neurotrophic receptor tyros... | NM_001369532.1 | 42.3% | .8% | 1_1417del;2462_2466delTAGGC |
| 6 | human | 4915 | NTRK2 | neurotrophic receptor tyros... | NM_001369533.1 | 42.3% | .8% | 1_1417del;2462_2466delTAGGC |
| 7 | human | 4915 | NTRK2 | neurotrophic receptor tyros... | XM_011518718.3 | 42.3% | .8% | 1_1417del;2462_2466delTAGGC |
| 8 | human | 4915 | NTRK2 | neurotrophic receptor tyros... | XM_017014752.1 | 42.3% | .8% | 1_1417del;2462_2466delTAGGC |
| 9 | human | 4915 | NTRK2 | neurotrophic receptor tyros... | XM_017014753.2 | 42.3% | .8% | 1_1417del;2462_2466delTAGGC |
| 10 | human | 4915 | NTRK2 | neurotrophic receptor tyros... | NM_006180.4 | 41.5% | .8% | 1_1465del;2510_2514delTAGGC |
| 11 | human | 4915 | NTRK2 | neurotrophic receptor tyros... | XM_005252001.3 | 41.5% | .8% | 1_1465del;2510_2514delTAGGC |
| 12 | human | 4915 | NTRK2 | neurotrophic receptor tyros... | XM_005252003.3 | 41.5% | .8% | 1_1465del;2510_2514delTAGGC |
| 13 | human | 4915 | NTRK2 | neurotrophic receptor tyros... | XM_005252004.2 | 41.5% | .8% | 1_1465del;2510_2514delTAGGC |
| 14 | human | 4915 | NTRK2 | neurotrophic receptor tyros... | XM_017014751.2 | 41.5% | .8% | 1_1465del;2510_2514delTAGGC |
| 15 | mouse | 18212 | Ntrk2 | neurotrophic tyrosine kinas... | NM_001025074.2 | 37.6% | .8% | (many diffs) |
| 16 | mouse | 18212 | Ntrk2 | neurotrophic tyrosine kinas... | NM_001282961.1 | 37.6% | .8% | (many diffs) |
| 17 | mouse | 18212 | Ntrk2 | neurotrophic tyrosine kinas... | XM_006517149.3 | 37.6% | .8% | (many diffs) |
| 18 | mouse | 18212 | Ntrk2 | neurotrophic tyrosine kinas... | XM_017315419.1 | 37.6% | .8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 81
- ORF end:
- 177
- ORF length:
- 96
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaattgg 61 cccagcctcc gttatcagca atgatgatga ctctgccagc ccactccatc acatctccaa 121 tgggagtaac actccatctt cttcggaagg tggcccagat gctgtcatta ttggaatgac 181 caagatccct gtcattgaaa atccccagta ctttggcatc accaacagtc agctcaagcc 241 agacacattt gttcagcaca tcaagcgaca taacattgtt ctgaaaaggg agctaggcga 301 aggagccttt ggaaaagtgt tcctagctga atgctataac ctctgtcctg agcaggacaa 361 gatcttggtg gcagtgaaga ccctgaagga tgccagtgac aatgcacgca aggacttcca 421 ccgtgaggcc gagctcctga ccaacctcca gcatgagcac atcgtcaagt tctatggcgt 481 ctgcgtggag ggcgaccccc tcatcatggt ctttgagtac atgaagcatg gggacctcaa 541 caagttcctc agggcacacg gccctgatgc cgtgctgatg gctgagggca acccgcccac 601 ggaactgacg cagtcgcaga tgctgcatat agcccagcag atcgccgcgg gcatggtcta 661 cctggcgtcc cagcacttcg tgcaccgcga tttggccacc aggaactgcc tggtcgggga 721 gaacttgctg gtgaaaaTCG GGGACTTTGG GATGTCCCGG GACGTGTACA GCACTGACTA 781 CTACAGGGTC GGTGGCCACA CAATGCTGCC CATTCGCTGG ATGCCTCCAG AGAGCATCAT 841 GTACAGGAAA TTCACGACGG AAAGCGACGT CTGGAGCCTG GGGGTCGTGT TGTGGGAGAT 901 TTTCACCTAT GGCAAACAGC CCTGGTACCA GCTGTCAAAC AATGAGGTGA TAGAGTGTAT 961 CACTCAGGGC CGAGTCCTGC AGCGACCCCG CACGTGCCCC CAGGAGGTGT ATGAGCTGAT 1021 GCTGGGGTGC TGGCAGCGAG AGCCCCACAT GAGGAAGAAC ATCAAGGGCA TCCATACCCT 1081 CCTTCAGAAC TTGGCCAAGG CATCTCCGGT CTACCTGGAC ATTCTAGGCT AGGACCCAGC 1141 TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA 1201 TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT 1261 ATCTTGTGGA AAGGACGACG CGGCAGTCTC CAGTCATTGA TTACGCGTTA AGTCgacaat 1321 caacctctgg attacaaaat ttgtgaaaga tt