Construct: ORF TRCN0000489361
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020552.1_s317c1
- DNA Barcode:
- AACGGTACGTTGGGCACGTCACCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PIP5K1B (8395)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489361
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | NM_003558.4 | 100% | 100% | |
| 2 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | XM_005252261.3 | 100% | 100% | |
| 3 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | XM_005252262.5 | 100% | 100% | |
| 4 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | XM_006717300.2 | 100% | 100% | |
| 5 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | XM_011519082.2 | 100% | 100% | |
| 6 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | XM_011519083.2 | 100% | 100% | |
| 7 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | XM_011519084.3 | 100% | 100% | |
| 8 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | XM_017015188.1 | 100% | 100% | |
| 9 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | XM_017015189.1 | 100% | 100% | |
| 10 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | XM_017015190.1 | 100% | 100% | |
| 11 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | NM_001278253.1 | 92.8% | 92.9% | 1503T>C;1506_1506delGins115 |
| 12 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | XM_006717301.1 | 92.8% | 92.9% | 1503T>C;1506_1506delGins115 |
| 13 | human | 8395 | PIP5K1B | phosphatidylinositol-4-phos... | XM_017015191.1 | 92.8% | 92.9% | 1503T>C;1506_1506delGins115 |
| 14 | mouse | 18719 | Pip5k1b | phosphatidylinositol-4-phos... | NM_008846.3 | 87.9% | 96.2% | (many diffs) |
| 15 | mouse | 18719 | Pip5k1b | phosphatidylinositol-4-phos... | XM_006526763.3 | 87.9% | 96.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1692
- ORF length:
- 1620
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtcttct gctgctgaaa atggagaggc agcacctgga aaacaaaatg 121 aagaaaaaac ctataaaaag actgcatcat ctgctattaa aggtgctatt cagctgggaa 181 taggatacac agtgggtaat ctcacttcca agccagaacg agatgttctt atgcaagact 241 tttatgtggt ggaaagtgtg ttcctaccca gcgaagggag caatctgacc ccagcacatc 301 actacccaga ctttagattt aagacatacg ctccattagc attccgatat ttcagagaac 361 tttttggtat caagcctgat gattacttgt attccatctg cagtgaacct ctaatagaac 421 tgtctaaccc tggagccagt ggatccttgt tttttgtgac cagtgatgat gaatttatca 481 tcaaaacagt tcagcacaaa gaagctgagt ttcttcagaa gctactgcca ggctattaca 541 tgaatttaaa ccagaatcca aggactcttt tgccaaaatt ttacggactg tattgtatgc 601 aatcaggagg cattaatatc aggattgtgg tgatgaacaa cgttttgcca cgctccatga 661 gaatgcactt tacatatgac ttgaaaggct caacgtataa gcgaagagca tcccgtaaag 721 agagagagaa atccaacccc acatttaagg acttagattt cctgcaagac atgcacgaag 781 ggttgtattt tgatacggaa acatacaacg cgcttatgaa aacacttcag agagactgcc 841 gggtgctaga aagcttcaag atcatggatt atagccttct gttgggaatt catttcctgg 901 accattccct caaagagaaa gaggaggaga ccccacaaaa tgtgcctgat gctaagcgga 961 ctgggatgca gaaggttctc tactcaacag ccatggaatc tatccagggt ccagggaaat 1021 ctggagatgg gataatcaca gagaacccag acacaatggg aggcattcca gctaaaagcc 1081 ataggggaga aaaactactt ttatttatgg gcattattga cattctgcaa tcatataggt 1141 taatgaagaa gttagaacat tcctggaaag ctcttgttta tgatggggac actgtttctg 1201 ttcatagacc aagcttttat gcagacagat ttcttaagtt catgaattcc agagttttca 1261 agaaaattca agctttgaag gcttcaccgt ctaagaaacg gtgcaattca atcgccgccc 1321 TAAAGGCCAC TTCACAGGAG ATTGTGTCCT CAATTAGCCA GGAATGGAAG GATGAGAAGC 1381 GGGATTTGCT GACTGAAGGA CAAAGTTTTA GCAGCCTTGA TGAAGAAGCC CTGGGATCCC 1441 GACACAGGCC AGACCTGGTC CCTAGCACTC CATCACTGTT TGAAGCTGCT TCCTTGGCAA 1501 CCACAATTTC ATCTTCTTCC TTATACGTCA ATGAGCACTA TCCACACGAC AGGCCTACAC 1561 TCTATTCAAA CAGCAAAGGG TTACCTTCCA GTTCAACATT TACCTTGGAA GAGGGGACCA 1621 TCTACTTGAC CGCTGAGCCC AACACTCTGG AAGTGCAGGA TGACAATGCT TCTGTGCTTG 1681 ACGTCTATTT ATAGGAACCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1741 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1801 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AACGGTACGT TGGGCACGTC 1861 ACCTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt