Transcript: Human NM_001278253.1

Homo sapiens phosphatidylinositol-4-phosphate 5-kinase type 1 beta (PIP5K1B), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-09-22
Taxon:
Homo sapiens (human)
Gene:
PIP5K1B (8395)
Length:
2682
CDS:
435..1943

Additional Resources:

NCBI RefSeq record:
NM_001278253.1
NBCI Gene record:
PIP5K1B (8395)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148929 GCTACTGCCAGGCTATTACA pXPR_003 TGG 466 31% 6 1.2692 PIP5K1B PIP5K1B 77086
2 BRDN0001149384 AATCTAAGTCCTTAAATGTG pXPR_003 GGG 671 44% 7 0.5217 PIP5K1B PIP5K1B 77084
3 BRDN0001146307 ATTCCAGCTAAAAGCCATAG pXPR_003 GGG 1010 67% 9 0.2386 PIP5K1B PIP5K1B 77085
4 BRDN0001147624 ATCACTGGTCACAAAAAACA pXPR_003 AGG 379 25% 6 -0.2839 PIP5K1B PIP5K1B 77083
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001278253.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000359201 ACATGCACGAAGGGTTGTATT pLKO_005 1132 CDS 100% 13.200 18.480 N PIP5K1B n/a
2 TRCN0000230744 ACGACAGGCCTACACTCTATT pLKO_005 1909 CDS 100% 13.200 18.480 N PIP5K1B n/a
3 TRCN0000230742 CCATTAGCATTCCGATATTTC pLKO_005 696 CDS 100% 13.200 18.480 N PIP5K1B n/a
4 TRCN0000359135 TACTACCCTATTCTATCATTT pLKO_005 2127 3UTR 100% 13.200 18.480 N PIP5K1B n/a
5 TRCN0000037860 CGTCTAAGAAACGGTGCAATT pLKO.1 1651 CDS 100% 10.800 15.120 N PIP5K1B n/a
6 TRCN0000037862 CGGAAACATACAACGCGCTTA pLKO.1 1159 CDS 100% 4.050 5.670 N PIP5K1B n/a
7 TRCN0000230743 ATGCAATCAGGAGGCATTAAT pLKO_005 960 CDS 100% 15.000 12.000 N PIP5K1B n/a
8 TRCN0000218779 CATCCACACACATGGTAAATT pLKO_005 2336 3UTR 100% 15.000 10.500 N PIP5K1B n/a
9 TRCN0000037859 CCAGGCTATTACATGAATTTA pLKO.1 891 CDS 100% 15.000 10.500 N PIP5K1B n/a
10 TRCN0000359136 CATCTGCAGTGAACCTCTAAT pLKO_005 758 CDS 100% 13.200 9.240 N PIP5K1B n/a
11 TRCN0000197135 GCTGACTGAAGGACAAAGTTT pLKO.1 1751 CDS 100% 5.625 3.938 N PIP5K1B n/a
12 TRCN0000037861 CGCTCCATTAGCATTCCGATA pLKO.1 692 CDS 100% 4.050 2.835 N PIP5K1B n/a
13 TRCN0000196843 GCAACATATGTAATCTGCTTA pLKO.1 2299 3UTR 100% 0.000 0.000 N PIP5K1B n/a
14 TRCN0000037863 GCACATCACTACCCAGACTTT pLKO.1 657 CDS 100% 4.950 2.970 N PIP5K1B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001278253.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489361 AACGGTACGTTGGGCACGTCACCT pLX_317 23.3% 92.8% 92.9% V5 (not translated due to prior stop codon) 1503T>C;1506_1506delGins115 n/a
2 ccsbBroadEn_11269 pDONR223 100% 91.3% 90.8% None (many diffs) n/a
3 ccsbBroad304_11269 pLX_304 0% 91.3% 90.8% V5 (many diffs) n/a
4 ccsbBroadEn_14892 pDONR223 0% 91.3% 90.8% None (many diffs) n/a
5 ccsbBroad304_14892 pLX_304 0% 91.3% 90.8% V5 (many diffs) n/a
6 TRCN0000474001 GACGGGCAACATTTCGGGGGATCA pLX_317 28.7% 91.3% 90.8% V5 (many diffs) n/a
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