Construct: ORF TRCN0000489372
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019945.1_s317c1
- DNA Barcode:
- CTCACCGCTACATTCTGCATATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HNF4A (3172)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489372
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3172 | HNF4A | hepatocyte nuclear factor 4... | NM_178850.3 | 99.9% | 99.7% | 1251_1252insG |
| 2 | human | 3172 | HNF4A | hepatocyte nuclear factor 4... | NM_001030004.3 | 92.5% | 91.6% | (many diffs) |
| 3 | human | 3172 | HNF4A | hepatocyte nuclear factor 4... | NM_001287184.2 | 92.4% | 91.1% | (many diffs) |
| 4 | human | 3172 | HNF4A | hepatocyte nuclear factor 4... | NM_178849.3 | 86.4% | 79.7% | (many diffs) |
| 5 | human | 3172 | HNF4A | hepatocyte nuclear factor 4... | NM_000457.4 | 84.8% | 79.5% | (many diffs) |
| 6 | human | 3172 | HNF4A | hepatocyte nuclear factor 4... | NM_001030003.3 | 80% | 72.7% | (many diffs) |
| 7 | human | 3172 | HNF4A | hepatocyte nuclear factor 4... | NM_001287182.2 | 79.9% | 72.4% | (many diffs) |
| 8 | human | 3172 | HNF4A | hepatocyte nuclear factor 4... | NM_001258355.2 | 78.4% | 71.1% | (many diffs) |
| 9 | human | 3172 | HNF4A | hepatocyte nuclear factor 4... | NM_175914.4 | 78.4% | 72.5% | (many diffs) |
| 10 | human | 3172 | HNF4A | hepatocyte nuclear factor 4... | XM_005260407.4 | 78.4% | 73.6% | (many diffs) |
| 11 | human | 3172 | HNF4A | hepatocyte nuclear factor 4... | NM_001287183.1 | 78.3% | 72.3% | (many diffs) |
| 12 | mouse | 15378 | Hnf4a | hepatic nuclear factor 4, a... | XM_006498787.1 | 77% | 78.7% | (many diffs) |
| 13 | mouse | 15378 | Hnf4a | hepatic nuclear factor 4, a... | NM_008261.3 | 76.4% | 77.6% | (many diffs) |
| 14 | mouse | 15378 | Hnf4a | hepatic nuclear factor 4, a... | XM_006498788.1 | 71.3% | 70.8% | (many diffs) |
| 15 | mouse | 15378 | Hnf4a | hepatic nuclear factor 4, a... | NM_001312907.1 | 71.3% | 71.3% | (many diffs) |
| 16 | mouse | 15378 | Hnf4a | hepatic nuclear factor 4, a... | NM_001312906.1 | 70.7% | 70.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1326
- ORF length:
- 1254
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcgactc tccaaaaccc tcgtcgacat ggacatggcc gactacagtg 121 ctgcactgga cccagcctac accaccctgg aatttgagaa tgtgcaggtg ttgacgatgg 181 gcaatgacac gtccccatca gaaggcacca acctcaacgc gcccaacagc ctgggtgtca 241 gcgccctgtg tgccatctgc ggggaccggg ccacgggcaa acactacggt gcctcgagct 301 gtgacggctg caagggcttc ttccggagga gcgtgcggaa gaaccacatg tactcctgca 361 gatttagccg gcagtgcgtg gtggacaaag acaagaggaa ccagtgccgc tactgcaggc 421 tcaagaaatg cttccgggct ggcatgaaga aggaagccgt ccagaatgag cgggaccgga 481 tcagcactcg aaggtcaagc tatgaggaca gcagcctgcc ctccatcaat gcgctcctgc 541 aggcggaggt cctgtcccga cagatcacct cccccgtctc cgggatcaac ggcgacattc 601 gggcgaagaa gattgccagc atcgcagatg tgtgtgagtc catgaaggag cagctgctgg 661 ttctcgttga gtgggccaag tacatcccag ctttctgcga gctccccctg gacgaccagg 721 tggccctgct cagagcccat gctggcgagc acctgctgct cggagccacc aagagatcca 781 tggtgttcaa ggacgtgctg ctcctaggca atgactacat tgtccctcgg cactgcccgg 841 agctggcgga gatgagccgg gtgtccatac gcatccttga cgagctggtg ctgcccttcc 901 aggagctgca gatcgatgac aatgagtatg cctacctcaa agccatcatc ttctttgacc 961 cagatgccaa ggggctgagc gatccaggga agatcaagcg gctgcgttcc caggtgcagg 1021 tgagcttgga ggactacatc aacgaccgcc agtatgactc gcgtggccgc tttggagagc 1081 tgctgctgct gctgcccacc ttgcagagca tcacctggca gatgatcgag cagatccagt 1141 tcatcaagct cTTCGGCATG GCCAAGATTG ACAACCTGTT GCAGGAGATG CTGCTGGGAG 1201 GTCCGTGCCA AGCCCAGGAG GGGCGGGGTT GGAGTGGGGA CTCCCCAGGA GACAGGCCTC 1261 ACACAGTGAG CTCACCCCTC AGCTCCTTGG CTTCCCCACT GTGCCGCTTT GGGCAAGTTG 1321 CTGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1381 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1441 GGCTTTATAT ATCTTGTGGA AAGGACGACT CACCGCTACA TTCTGCATAT CGACGCGTTA 1501 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt