Construct: ORF TRCN0000489375
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019597.1_s317c1
- DNA Barcode:
- GGTTACAGCATCAAGTGAACATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SRPK3 (26576)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489375
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26576 | SRPK3 | SRSF protein kinase 3 | NM_001170760.2 | 99.9% | 99.8% | 1698_1699insG |
| 2 | human | 26576 | SRPK3 | SRSF protein kinase 3 | NM_014370.4 | 99.7% | 99.6% | 774_776delGCA;1701_1702insG |
| 3 | human | 26576 | SRPK3 | SRSF protein kinase 3 | NM_001170761.1 | 94.1% | 94% | 948_949ins99;1599_1600insG |
| 4 | mouse | 56504 | Srpk3 | serine/arginine-rich protei... | NM_019684.2 | 85.7% | 94.1% | (many diffs) |
| 5 | mouse | 56504 | Srpk3 | serine/arginine-rich protei... | XM_006528172.3 | 82.1% | 90.2% | (many diffs) |
| 6 | mouse | 56504 | Srpk3 | serine/arginine-rich protei... | XM_017318579.1 | 37.9% | 40.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1767
- ORF length:
- 1701
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagaat 61 tcaccatgag cgccagcacg ggcggtggtg gggacagcgg cggcagcggc ggcagtagca 121 gcagctcaca ggcctcctgc gggcccgagt cctcgggctc cgaactagcc ctggccacac 181 cggtgcctca gatgctgcag ggccttctgg gctccgacga cgaggaacag gaagacccca 241 aagactactg caagggcggc taccaccctg tgaagatcgg cgacgtgttc aatgggcggt 301 accacgtggt gcgcaaactg ggctggggcc acttctccac cgtctggctc tgctgggaca 361 tccagcgcaa gcgctttgtg gccctcaaag tggtgaagag tgcggggcat tacacggaga 421 cagctgtgga tgagatcaag ctcctgaaat gtgtccggga cagcgacccc agtgacccca 481 aaagagagac cattgtccag ctcattgatg acttcaggat ctcaggagtc aatggagtcc 541 atgtgtgcat ggtgctggag gtgctgggcc accagctcct caaatggatc atcaagtcca 601 actaccaggg cctgcccgtg ccctgcgtga agagcatcgt gaggcaggtg ctgcacggcc 661 tggactacct ccacaccaag tgcaagatca tccacacgga catcaagccc gagaacatct 721 tgctgtgtgt gggggacgct tacatcaggc gcctggctgc cgaggccacg gagtggcaac 781 aggcaggggc gccgcccccc tcccgctcca tagtcagcac tgccccccag gaggtcttga 841 ccggtaagct gtccaaaaac aagaggaaga agatgaggcg caaacggaaa cagcagaagc 901 ggctgctgga ggagcggctg cgggacctgc agaggctgga ggccatggag gctgccaccc 961 aggctgagga ctctggcttg agactagacg ggggcagcgg ctccacatcc tcttcaggct 1021 gtcaccccgg gggcgccaga gcaggtccct ccccagcctc ttcctccccc gccccagggg 1081 gcggccgtag cctcagcgcg ggctcacaga cctcaggctt ctccggctcc ctcttctctc 1141 ctgcctcctg ctccatcctc tccggctcgt ccaatcagcg agagaccggg ggcctcctgt 1201 cgcctagcac accattcggt gcctcgaacc tcctggtgaa ccccctggag ccccaaaatg 1261 cagataagat caagatcaag atcgcagacc tgggcaacgc ctgctgggtg cacaagcact 1321 tcacggaaga catccagact cggcagtacc gggccgtcga ggtgctgatc ggcgccgaat 1381 acggcccccc ggcagacatc tggagcacag cctgcatggc cTTCGAGCTG GCCACTGGTG 1441 ACTACCTGTT CGAGCCGCAT TCTGGAGAAG ACTACAGTCG TGATGAGGAC CACATCGCTC 1501 ACATAGTGGA GCTTCTGGGG GACATCCCCC CAGCCTTCGC CCTCTCAGGC CGCTATTCCC 1561 GGGAGTTCTT CAACCGGAGA GGAGAGCTGC GGCACATCCA CAATCTCAAG CACTGGGGCC 1621 TGTACGAGGT ACTCATGGAA AAGTACGAGT GGCCCCTAGA GCAGGCCACA CAGTTCAGCG 1681 CCTTTCTGCT GCCCATGATG GAGTACATCC CCGAAAAGCG GGCCAGTGCC GCTGACTGCC 1741 TCCAGCACCC CTGGCTCAAC CCCGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1801 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1861 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG GTTACAGCAT 1921 CAAGTGAACA TCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1981 att