Construct: ORF TRCN0000489381
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021683.1_s317c1
- DNA Barcode:
- TCACTGGGCAATGCCACGACGAAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- IRAK1 (3654)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489381
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3654 | IRAK1 | interleukin 1 receptor asso... | NM_001569.4 | 94% | 97.4% | (many diffs) |
| 2 | human | 3654 | IRAK1 | interleukin 1 receptor asso... | NM_001025242.2 | 89.8% | 93.3% | (many diffs) |
| 3 | human | 3654 | IRAK1 | interleukin 1 receptor asso... | NM_001025243.2 | 86.8% | 90.5% | (many diffs) |
| 4 | human | 3654 | IRAK1 | interleukin 1 receptor asso... | XM_005274668.4 | 86.7% | 90.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2157
- ORF length:
- 2091
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcatggc gggggggccg ggcccggggg agcccgcagc ccccggcgcc cagcacttct 121 tgtacgaggt gccgccctgg gtcatgtgcc gcttctacaa agtgatggac gccctggagc 181 ccgccgactg gtgccagttc gccgccctga tcgtgcgcga ccagaccgag ctgcggctgt 241 gcgagcgctc cgggcagcgc acggccagcg tcctgtggcc ctggatcaac cgcaacgccc 301 gtgtggccga cctcgtgcac atcctcacgc acctgcagct gctccgtgcg cgggacatca 361 tcacagcctg gcaccctccc gccccgcttc cgtccccagg caccactgcc ccgaggccca 421 gcagcatccc tgcacccgcc gaggccgagg cctggagccc ccggaagttg ccatcctcag 481 cctccacctt cctctcccca gcttttccag gctcccagac ccattcaggg cctgagctcg 541 gcctggttcc aagccctgct tccctgtggc ctccaccgcc atctccagcc ccttcttcta 601 ccaagccagg cccagagagc tcagtgtccc tcctgcaggg agcccgcccc tctccgtttt 661 gctggcccct ctgtgagatt tcccggggca cccacaactt ctcggaggag ctcaagatcg 721 gggagggtgg ctttgggtgc gtgtaccggg cggtgatgag gaacacggtg tatgctgtga 781 agaggctgaa ggagaacgct gacctggagt ggactgcagt gaagcagagc ttcctgaccg 841 aggtggagca gctgtccagg tttcgtcacc caaacattgt ggactttgct ggctactgtg 901 ctcagaacgg cttctactgc ctggtgtacg gcttcctgcc caacggctcc ctggaggacc 961 gtctccactg ccagacccag gcctgcccac ctctctcctg gcctcagcga ctggacatcc 1021 ttctgggtac agcccgggca attcagtttc tacatcagga cagccccagc ctcatccatg 1081 gagacatcaa gagttccaac gtccttctgg atgagaggct gacacccaag ctgggagact 1141 ttggcctggc ccggttcagc cgctttgccg ggtccagccc cagccagagc agcatggtgg 1201 cccggacaca gacagtgcgg ggcaccctgg cctacctgcc cgaggagtac atcaagacgg 1261 gaaggctggc tgtggacacg gacaccttca gctttggggt ggtagtgcta gagaccttgg 1321 ctggtcagag ggctgtgaag acgcacggtg ccaggaccaa gtatctgaaa gacctggtgg 1381 aagaggaggc tgaggaggct ggagtggctt tgagaagcac ccagagcaca ctgcaagcag 1441 gtctggctgc agatgcctgg gctgctccca tcgccatgca gatctacaag aagcgcctgg 1501 gccagctggc ctgctgctgc ctgcaccgcc gggccaaaag gaggcctcct atgacccagg 1561 tgtacgagag gctagagaag ctgcaggcag tggtggcggg ggtgcccggg catttggagg 1621 ccgccagctg catcccccct tccccgcagg agaactccta cgtgtccagc actggcagag 1681 cccacagtgg ggctgctcca tggcagcccc tggcagcgcc atcaggagcc agtgcccagg 1741 cagcagagca gctgcagaga ggccccaacc agcccgtgga gagtgacgag agcctaggcg 1801 gcctctctgc tgccctgcgc tcctggcact tgactccaag ctgccctctg gacccagcac 1861 ccctcaggga ggccggctgt ccTCAGGGGG ACACGGCAGG AGAATCGAGC TGGGGGAGTG 1921 GCCCAGGATC CCGGCCCACA GCCGTGGAAG GACTGGCCCT TGGCAGCTCT GCATCATCAT 1981 CGTCAGAGCC ACCGCAGATT ATCATCAACC CTGCCCGACA GAAGATGGTC CAGAAGCTGG 2041 CCCTGTACGA GGATGGGGCC CTGGACAGCC TGCAGCTGCT GTCGTCCAGC TCCCTCCCAG 2101 GCTTGGGCCT GGAACAGGAC AGGCAGGGGC CCGAAGAAAG TGATGAATTT CAGAGCTGAG 2161 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2221 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2281 TTTATATATC TTGTGGAAAG GACGATCACT GGGCAATGCC ACGACGAACA CGCGTTAAGT 2341 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt