Transcript: Human NM_001025242.2

Homo sapiens interleukin 1 receptor associated kinase 1 (IRAK1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-21
Taxon:
Homo sapiens (human)
Gene:
IRAK1 (3654)
Length:
3491
CDS:
91..2139

Additional Resources:

NCBI RefSeq record:
NM_001025242.2
NBCI Gene record:
IRAK1 (3654)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146412 ACACGGTGTATGCTGTGAAG pXPR_003 AGG 714 35% 5 0.8565 IRAK1 IRAK1 76421
2 BRDN0001145661 ATTTATCCCACAGAAAGACC pXPR_003 TGG 1306 64% 11 0.5449 IRAK1 IRAK1 76420
3 BRDN0001145800 AGGAGTACATCAAGACGGGA pXPR_003 AGG 1194 58% 9 0.4933 IRAK1 IRAK1 76418
4 BRDN0001147615 GATCAACCGCAACGCCCGTG pXPR_003 TGG 235 11% 2 0.3811 IRAK1 IRAK1 76419
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001025242.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000121239 GAACACGGTGTATGCTGTGAA pLKO.1 786 CDS 100% 4.950 6.930 N IRAK1 n/a
2 TRCN0000342718 GAACACGGTGTATGCTGTGAA pLKO_005 786 CDS 100% 4.950 6.930 N IRAK1 n/a
3 TRCN0000121139 CCGCTTCTACAAAGTGATGGA pLKO.1 174 CDS 100% 2.640 3.696 N IRAK1 n/a
4 TRCN0000121240 CCAGCACTTCTTGTACGAGGT pLKO.1 135 CDS 100% 2.160 3.024 N IRAK1 n/a
5 TRCN0000000545 CGGGCAATTCAGTTTCTACAT pLKO.1 1060 CDS 100% 4.950 3.960 N IRAK1 n/a
6 TRCN0000121140 TCACCCAAACATTGTGGACTT pLKO.1 891 CDS 100% 4.050 3.240 N IRAK1 n/a
7 TRCN0000121305 AGTTCCAACGTCCTTCTGGAT pLKO.1 1117 CDS 100% 2.640 2.112 N IRAK1 n/a
8 TRCN0000000543 CCCTCCTACCTGCTTACAATT pLKO.1 2577 3UTR 100% 13.200 9.240 N IRAK1 n/a
9 TRCN0000342655 CCCTCCTACCTGCTTACAATT pLKO_005 2577 3UTR 100% 13.200 9.240 N IRAK1 n/a
10 TRCN0000121238 CATCGCCATGCAGATCTACAA pLKO.1 1494 CDS 100% 4.950 3.465 N IRAK1 n/a
11 TRCN0000195319 CTCATCCATGGAGACATCAAG pLKO.1 1096 CDS 100% 4.950 3.465 N IRAK1 n/a
12 TRCN0000121138 GCCACCGCAGATTATCATCAA pLKO.1 1968 CDS 100% 4.950 3.465 N IRAK1 n/a
13 TRCN0000342654 GCCACCGCAGATTATCATCAA pLKO_005 1968 CDS 100% 4.950 3.465 N IRAK1 n/a
14 TRCN0000121237 GTATGCTGTGAAGAGGCTGAA pLKO.1 795 CDS 100% 4.050 2.835 N IRAK1 n/a
15 TRCN0000000546 CATTGTGGACTTTGCTGGCTA pLKO.1 900 CDS 100% 2.640 1.848 N IRAK1 n/a
16 TRCN0000000547 AGGAGTACATCAAGACGGGAA pLKO.1 1268 CDS 100% 2.160 1.512 N IRAK1 n/a
17 TRCN0000199361 CCTTGGCAGCTCTGCATCATC pLKO.1 1938 CDS 100% 1.650 1.155 N IRAK1 n/a
18 TRCN0000121304 CCCGACAGAAGATGGTCCAGA pLKO.1 1994 CDS 100% 0.880 0.616 N IRAK1 n/a
19 TRCN0000199382 CACCCACAACTTCTCGGAGGA pLKO.1 714 CDS 100% 0.720 0.504 N IRAK1 n/a
20 TRCN0000000544 GCCCGAAGAAAGTGATGAATT pLKO.1 2109 CDS 100% 0.000 0.000 N IRAK1 n/a
21 TRCN0000121137 GCTGAAGTAGGAGGATCATTT pLKO.1 2794 3UTR 100% 13.200 7.920 N IRAK1 n/a
22 TRCN0000342719 GCTGAAGTAGGAGGATCATTT pLKO_005 2794 3UTR 100% 13.200 7.920 N IRAK1 n/a
23 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 2628 3UTR 100% 4.950 2.475 Y ERAP2 n/a
24 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 2629 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001025242.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489710 TGTCAATGGTAATAGGAATCCGTG pLX_317 13.3% 95.7% 95.7% V5 (not translated due to prior stop codon) 1536_1537ins90 n/a
2 TRCN0000491566 TGCCAGATTATATCCTCATCCTCG pLX_317 14% 95.7% 95.6% V5 1536_1537ins90;2046_2047insG n/a
3 TRCN0000489613 CACCTTAGGGTACTTGCCGCTGTA pLX_317 14.4% 93.5% 93.3% V5 (not translated due to prior stop codon) (many diffs) n/a
4 TRCN0000488845 GCTCGCGGTGCTCTGCTTGCCGTG pLX_317 10.8% 93.4% 93.2% V5 (many diffs) n/a
5 TRCN0000489381 TCACTGGGCAATGCCACGACGAAC pLX_317 13.6% 89.8% 93.3% V5 (not translated due to prior stop codon) (many diffs) n/a
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