Construct: ORF TRCN0000489399
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020453.1_s317c1
- DNA Barcode:
- CGAACATATCTCTTAATTACTCTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- EIF2AK3 (9451)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489399
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9451 | EIF2AK3 | eukaryotic translation init... | NM_004836.7 | 99.9% | 99.8% | 497A>G;2110G>T |
| 2 | human | 9451 | EIF2AK3 | eukaryotic translation init... | NM_001313915.1 | 86.4% | 86.2% | 0_1ins453;44A>G;1657G>T |
| 3 | human | 9451 | EIF2AK3 | eukaryotic translation init... | XM_017005376.2 | 79.5% | 79.4% | 0_1ins684;1426G>T |
| 4 | mouse | 13666 | Eif2ak3 | eukaryotic translation init... | NM_010121.3 | 82.3% | 87.6% | (many diffs) |
| 5 | mouse | 13666 | Eif2ak3 | eukaryotic translation init... | XM_011241202.2 | 82.3% | 87.5% | (many diffs) |
| 6 | mouse | 13666 | Eif2ak3 | eukaryotic translation init... | XM_006505501.1 | 65.3% | 66.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3420
- ORF length:
- 3348
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagcgc gccatcagcc cggggctgct ggtacgggcg ctgctgctgc 121 tgctgctgct gctggggctc gcggcaagga cggtggccgc ggggcgcgcc cgtggcctcc 181 cagcgccgac ggcggaggcg gcgttcggcc tcggggcggc cgctgctccc acctcagcga 241 cgcgagtacc ggcggcgggc gccgtggctg cggccgaggt gactgtggag gacgctgagg 301 cgctgccggc agccgcggga gagcaggagc ctcggggtcc ggaaccagac gatgagacag 361 agttgcgacc gcgcggcagg tcattagtaa ttatcagcac tttagatggg agaattgctg 421 ccttggatcc tgaaaatcat ggtaaaaagc agtgggattt ggatgtggga tccggttcct 481 tggtgtcatc cagccttagc aaaccagagg tatttgggaa taagatgatc attccttccc 541 tggatggagc cctcttccag tgggaccgag accgtgaaag catggaaaca gttcctttca 601 cagttgaatc acttcttgaa tcttcttata aatttggaga tgatgttgtt ttggttggag 661 gaaaatctct gactacatat ggactcagtg catatagtgg aaaggtgagg tatatctgtt 721 cagctctggg ttgtcgccaa tgggatagtg acgaaatgga acaagaggaa gacatcctgc 781 ttctacagcg tacccaaaaa actgttagag ctgtcggacc tcgcagtggc aatgagaagt 841 ggaatttcag tgttggccac tttgaacttc ggtatattcc agacatggaa acgagagccg 901 gatttattga aagcaccttt aagcccaatg agaacacaga agagtctaaa attatttcag 961 atgtggaaga acaggaagct gccataatgg acatagtgat aaaggtttcg gttgctgact 1021 ggaaagttat ggcattcagt aagaagggag gacatctgga atgggagtac cagttttgta 1081 ctccaattgc atctgcctgg ttacttaagg atgggaaagt cattcccatc agtctttttg 1141 atgatacaag ttatacatct aatgatgatg ttttagaaga tgaagaagac attgtagaag 1201 ctgccagagg agccacagaa aacagtgttt acttgggaat gtatagaggc cagctgtatc 1261 tgcagtcatc agtcagaatt tcagaaaagt ttccttcaag tcccaaggct ttggaatctg 1321 tcactaatga aaacgcaatt attcctttac caacaatcaa atggaaaccc ttaattcatt 1381 ctccttccag aactcctgtc ttggtaggat ctgatgaatt tgacaaatgt ctcagtaatg 1441 ataagttttc tcatgaagaa tatagtaatg gtgcactttc aatcttgcag tatccatatg 1501 ataatggtta ttatctacca tactacaaga gggagaggaa caaacgaagc acacagatta 1561 cagtcagatt cctcgacaac ccacattaca acaagaatat ccgcaaaaag gatcctgttc 1621 ttcttttaca ctggtggaaa gaaatagttg caacgatttt gttttgtatc atagcaacaa 1681 cgtttattgt gcgcaggctt ttccatcctc atcctcacag gcaaaggaag gagtctgaaa 1741 ctcagtgtca aactgaaaat aaatatgatt ctgtaagtgg tgaagccaat gacagtagct 1801 ggaatgacat aaaaaactct ggatatatat cacgatatct aactgatttt gagccaattc 1861 aatgcctggg acgtggtggc tttggagttg tttttgaagc taaaaacaaa gtagatgact 1921 gcaattatgc tatcaagagg atccgtctcc ccaataggga attggctcgg gaaaaggtaa 1981 tgcgagaagt taaagcctta gccaagcttg aacacccggg cattgttaga tatttcaatg 2041 cctggctcga agcaccacca gagaagtggc aagaaaagat ggatgaaatt tggctgaaag 2101 atgaaagcac agactggcca ctcagctctc ctagcccaat ggatgcacca tcagttaaaa 2161 tacgcagaat ggatcctttc tctacaaaag aacatattga aatcatagct ccttcaccac 2221 aaagaagcag gtctttttca gtagggattt cctgtgacca gacaagttca tctgagagcc 2281 agttctcacc actggaattc tcaggaatgg accatgagga catcagtgag tcagtggatg 2341 cagcatacaa cctccaggac agttgcctta cagactgtga tgtggaagat gggactatgg 2401 atggcaatga tgaggggcac tcctttgaac tttgtccttc tgaagcttct ccttatgtaa 2461 ggtcaaggga gagaacctcc tcttcaatag tatttgaaga ttctggctgt gataatgctt 2521 ccagtaaaga agagccgaaa actaatcgat tgcatattgg caaccattgt gctaataaac 2581 taactgcttt caagcccacc agtagcaaat cttcttctga agctacattg tctatttctc 2641 ctccaagacc aaccacttta agtttagatc tcactaaaaa caccacagaa aaactccagc 2701 ccagttcacc aaaggtgtat ctttacattc aaatgcagct gtgcagaaaa gaaaacctca 2761 aagactggat gaatggacga tgtaccatag aggagagaga gaggagcgtg tgtctgcaca 2821 tcttcctgca gatcgcagag gcagtggagt ttcttcacag taaaggactg atgcacaggg 2881 acctcaagcc atccaacata ttctttacaa tggatgatgt ggtcaaggtt ggagactttg 2941 ggttagtgac tgcaatggac caggatgagg aagagcagac ggttctgacc ccaatgccag 3001 cttatgccag acacacagga caagtaggga ccaaactgta tatgagccca gagcagattc 3061 atggaaacag ctattctcat aaagtggaca tcttttcttt aggcctgatt ctatttGAAT 3121 TGCTGTATCC ATTCAGCACT CAGATGGAGA GAGTCAGGAC CTTAACTGAT GTAAGAAATC 3181 TCAAATTTCC ACCATTATTT ACTCAGAAAT ATCCTTGTGA GTACGTGATG GTTCAAGACA 3241 TGCTCTCTCC ATCCCCCATG GAACGACCTG AAGCTATAAA CATCATTGAA AATGCTGTAT 3301 TTGAGGACTT GGACTTTCCA GGAAAAACAG TGCTCAGACA GAGGTCTCGC TCCTTGAGTT 3361 CATCGGGAAC AAAACATTCA AGACAGTCCA ACAACTCCCA TAGCCCTTTG CCAAGCAATT 3421 AGGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 3481 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT GAAAGTATTT CGATTTCTTG 3541 GCTTTATATA TCTTGTGGAA AGGACGACGA ACATATCTCT TAATTACTCT CACGCGTTAA 3601 GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t