Transcript: Mouse XM_011241202.2

PREDICTED: Mus musculus eukaryotic translation initiation factor 2 alpha kinase 3 (Eif2ak3), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Eif2ak3 (13666)
Length:
4537
CDS:
244..3585

Additional Resources:

NCBI RefSeq record:
XM_011241202.2
NBCI Gene record:
Eif2ak3 (13666)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145848 GAATATACCGAAGTTCAAAG pXPR_003 TGG 779 23% 5 0.5686 Eif2ak3 EIF2AK3 77166
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011241202.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000028772 GCCACTTTGAACTTCGGTATA pLKO.1 1016 CDS 100% 10.800 15.120 N Eif2ak3 n/a
2 TRCN0000321805 GCCACTTTGAACTTCGGTATA pLKO_005 1016 CDS 100% 10.800 15.120 N Eif2ak3 n/a
3 TRCN0000028759 CCATACGATAACGGTTACTAT pLKO.1 1654 CDS 100% 5.625 7.875 N Eif2ak3 n/a
4 TRCN0000321873 GTGACCCATCTGCACTAATTT pLKO_005 4013 3UTR 100% 15.000 12.000 N Eif2ak3 n/a
5 TRCN0000262381 CACTTTGAACTTCGGTATATT pLKO_005 1018 CDS 100% 15.000 10.500 N EIF2AK3 n/a
6 TRCN0000028787 CCATGAGTTCATCTGGAACAA pLKO.1 3515 CDS 100% 4.950 3.465 N Eif2ak3 n/a
7 TRCN0000321872 CCATGAGTTCATCTGGAACAA pLKO_005 3515 CDS 100% 4.950 3.465 N Eif2ak3 n/a
8 TRCN0000028831 CCTCTACTGTTCACTCAGAAA pLKO.1 3352 CDS 100% 4.950 3.465 N Eif2ak3 n/a
9 TRCN0000321806 CCTCTACTGTTCACTCAGAAA pLKO_005 3352 CDS 100% 4.950 3.465 N Eif2ak3 n/a
10 TRCN0000028799 GCCTGTTTGATGATACAAGTT pLKO.1 1292 CDS 100% 4.950 3.465 N Eif2ak3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011241202.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000492157 AAACCCTACTTTCGTCCTAATACG pLX_317 10.9% 82.3% 87.5% V5 (many diffs) n/a
2 TRCN0000491412 ACACCGATACTCGCACGTATCTCC pLX_317 10.1% 82.3% 87.5% V5 (many diffs) n/a
3 TRCN0000489399 CGAACATATCTCTTAATTACTCTC pLX_317 9.3% 82.3% 87.5% V5 (not translated due to prior stop codon) (many diffs) n/a
4 TRCN0000488883 GCACCGGTCTTAAATTCAATCTAG pLX_317 8.5% 82.3% 87.5% V5 (not translated due to prior stop codon) (many diffs) n/a
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