Construct: ORF TRCN0000489400
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020449.1_s317c1
- DNA Barcode:
- CTCGCCACCCATCAACGGCCTCCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NEK2 (4751)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489400
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4751 | NEK2 | NIMA related kinase 2 | NM_002497.4 | 99.7% | 100% | (many diffs) |
| 2 | human | 4751 | NEK2 | NIMA related kinase 2 | XM_005273147.2 | 97.9% | 98.2% | (many diffs) |
| 3 | human | 4751 | NEK2 | NIMA related kinase 2 | NM_001204183.1 | 85.5% | 83.6% | (many diffs) |
| 4 | human | 4751 | NEK2 | NIMA related kinase 2 | NM_001204182.1 | 85.4% | 83.8% | (many diffs) |
| 5 | mouse | 18005 | Nek2 | NIMA (never in mitosis gene... | NM_010892.3 | 83% | 88% | (many diffs) |
| 6 | mouse | 18005 | Nek2 | NIMA (never in mitosis gene... | XM_006497123.3 | 81.7% | 86.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1407
- ORF length:
- 1335
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgccttcc cgggcggagg actatgaagt gttgtacacc attggcacag 121 gctcctacgg ccgctgccag aagatccgga ggaagagtga tggcaagata ttagtttgga 181 aagaacttga ctatggctcc atgacagaag ctgagaaaca gatgcttgtt tctgaagtga 241 atttgcttcg tgaactgaaa catccaaaca tcgttcgtta ctatgatcgg attattgacc 301 ggaccaatac aacactgtac attgtaatgg aatattgtga aggaggggat ctggctagtg 361 taattacaaa gggaaccaag gaaaggcaat acttagatga agagtttgtt cttcgagtga 421 tgactcagtt gactctggcc ctgaaggaat gccacagacg aagtgatggt ggtcataccg 481 tattgcatcg ggatctgaaa ccagccaatg ttttcctgga tggcaagcaa aacgtcaagc 541 ttggagactt tgggctagct agaatattaa accacgacac gagttttgca aaaacatttg 601 ttggcacacc ttattacatg tctcctgaac aaatgaatcg catgtcctac aatgagaaat 661 cagatatctg gtcattgggc tgcttgctgt atgagttatg tgcattaatg cctccattta 721 cagcttttag ccagaaagaa ctcgctggga aaatcagaga aggcaaattc aggcgaattc 781 cataccgtta ctctgatgaa ttgaatgaaa ttattacgag gatgttaaac ttaaaggatt 841 accatcgacc ttctgttgaa gaaattcttg agaacccttt aatagcagat ttggttgcag 901 acgagcaaag aagaaatctt gagagaagag ggcgacaatt aggagagcca gaaaaatcgc 961 aggattccag ccctgtattg agtgagctga aactgaagga aattcagtta caggagcgag 1021 agcgagctct caaagcaaga gaagaaagat tGGAGCAGAA AGAACAGGAG CTTTGTGTTC 1081 GTGAGAGACT AGCAGAGGAC AAACTGGCTA GAGCAGAAAA TCTGTTGAAG AACTACAGCT 1141 TGCTAAAGGA ACGGAAGTTC CTGTCTCTGG CAAGTAATCC AGAACTTCTT AATCTTCCAT 1201 CCTCAGTAAT TAAGAAGAAA GTTCATTTCA GTGGGGAAAG TAAAGAGAAC ATCATGAGGA 1261 GTGAGAATTC TGAGAGTCAG CTCACATCTA AGTCCAAGTG CAAGGACCTG AAGAAAAGGC 1321 TTCACGCTGC CCAGCTGCGT GCTCAAGCCC TGTCAGATAT TGAGAAAAAT TACCAACTGA 1381 AAAGCAGGCA GATCCTGGGC ATGCGCTAGG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 1441 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1501 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACTCGC 1561 CACCCATCAA CGGCCTCCTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1621 tgaaagatt