Construct: ORF TRCN0000489401
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020418.1_s317c1
- DNA Barcode:
- ATTTTTCATCCTTTACTTGGCCGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TBK1 (29110)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489401
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29110 | TBK1 | TANK binding kinase 1 | NM_013254.4 | 99.9% | 100% | 978T>A |
| 2 | human | 29110 | TBK1 | TANK binding kinase 1 | XM_005268809.1 | 99.9% | 100% | 978T>A |
| 3 | human | 29110 | TBK1 | TANK binding kinase 1 | XM_005268810.1 | 99.9% | 100% | 978T>A |
| 4 | human | 29110 | TBK1 | TANK binding kinase 1 | XR_001748674.2 | 69.5% | (many diffs) | |
| 5 | mouse | 56480 | Tbk1 | TANK-binding kinase 1 | NM_019786.4 | 86.3% | 94.1% | (many diffs) |
| 6 | mouse | 56480 | Tbk1 | TANK-binding kinase 1 | XR_001779563.1 | 50.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 84
- ORF end:
- 2271
- ORF length:
- 2187
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggcccccttc accatgcaga gcacttctaa tcatctgtgg cttttatctg 121 atattttagg ccaaggagct actgcaaatg tctttcgtgg aagacataag aaaactggtg 181 atttatttgc tatcaaagta tttaataaca taagcttcct tcgtccagtg gatgttcaaa 241 tgagagaatt tgaagtgttg aaaaaactca atcacaaaaa tattgtcaaa ttatttgcta 301 ttgaagagga gacaacaaca agacataaag tacttattat ggaattttgt ccatgtggga 361 gtttatacac tgttttagaa gaaccttcta atgcctatgg actaccagaa tctgaattct 421 taattgtttt gcgagatgtg gtgggtggaa tgaatcatct acgagagaat ggtatagtgc 481 accgtgatat caagccagga aatatcatgc gtgttatagg ggaagatgga cagtctgtgt 541 acaaactcac agattttggt gcagctagag aattagaaga tgatgagcag tttgtttctc 601 tgtatggcac agaagaatat ttgcaccctg atatgtatga gagagcagtg ctaagaaaag 661 atcatcagaa gaaatatgga gcaacagttg atctttggag cattggggta acattttacc 721 atgcagctac tggatcactg ccatttagac cctttgaagg gcctcgtagg aataaagaag 781 tgatgtataa aataattaca ggaaagcctt ctggtgcaat atctggagta cagaaagcag 841 aaaatggacc aattgactgg agtggagaca tgcctgtttc ttgcagtctt tctcggggtc 901 ttcaggttct acttacccct gttcttgcaa acatccttga agcagatcag gaaaagtgtt 961 ggggttttga ccagtttttt gcagaaacta gtgatatact tcaccgaatg gtaattcatg 1021 ttttttcgct acaacaaatg acagctcata agatttatat acatagctat aatactgcta 1081 ctatatttca tgaactggta tataaacaaa ccaaaattat ttcttcaaat caagaactta 1141 tctacgaagg gcgacgctta gtcttagaac ctggaaggct ggcacaacat ttccctaaaa 1201 ctactgagga aaaccctata tttgtagtaa gccgggaacc tctgaatacc ataggattaa 1261 tatatgaaaa aatttccctc cctaaagtac atccacgtta tgatttagac ggggatgcta 1321 gcatggctaa ggcaataaca ggggttgtgt gttatgcctg cagaattgcc agtaccttac 1381 tgctttatca ggaattaatg cgaaagggga tacgatggct gattgaatta attaaagatg 1441 attacaatga aactgttcac aaaaagacag aagttgtgat cacattggat ttctgtatca 1501 gaaacattga aaaaactgtg aaagtatatg aaaagttgat gaagatcaac ctggaagcgg 1561 cagagttagg tgaaatttca gacatacaca ccaaattgtt gagactttcc agttctcagg 1621 gaacaataga aaccagtctt caggatatcg acagcagatt atctccaggt ggatcactgg 1681 cagacgcatg ggcacatcaa gaaggcactc atccgaaaga cagaaatgta gaaaaactac 1741 aagtcctgtt aaattgcatg acagagattt actatcagtt caaaaaagac aaagcagaac 1801 gtagattagc ttataatgaa gaacaaatcc acaaatttga taagcaaaaa ctgtattacc 1861 atgccacaaa agctatgacg cactttacag atgaatgtgt taaaaagtat gaggcatttt 1921 tGAATAAGTC AGAAGAATGG ATAAGAAAGA TGCTTCATCT TAGGAAACAG TTATTATCGC 1981 TGACTAATCA GTGTTTTGAT ATTGAAGAAG AAGTATCAAA ATATCAAGAA TATACTAATG 2041 AGTTACAAGA AACTCTGCCT CAGAAAATGT TTACAGCTTC CAGTGGAATC AAACATACCA 2101 TGACCCCAAT TTATCCAAGT TCTAACACAT TAGTAGAAAT GACTCTTGGT ATGAAGAAAT 2161 TAAAGGAAGA GATGGAAGGG GTGGTTAAAG AACTTGCTGA AAATAACCAC ATTTTAGAAA 2221 GGTTTGGCTC TTTAACCATG GATGGTGGCC TTCGCAACGT TGACTGTCTT TAGTCTAGAA 2281 AGGGTGGGCG CGCCGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2341 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2401 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATTTTTCATC CTTTACTTGG 2461 CCGTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt