Construct: ORF TRCN0000489402
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020261.1_s317c1
- DNA Barcode:
- TAGACTCGTCGCGTCGTCTGCCGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- STK17B (9262)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489402
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9262 | STK17B | serine/threonine kinase 17b | NM_004226.4 | 100% | 100% | |
| 2 | human | 9262 | STK17B | serine/threonine kinase 17b | XM_011512168.3 | 100% | 100% | |
| 3 | human | 9262 | STK17B | serine/threonine kinase 17b | XM_011512169.1 | 100% | 100% | |
| 4 | human | 9262 | STK17B | serine/threonine kinase 17b | XM_011512170.1 | 100% | 100% | |
| 5 | human | 9262 | STK17B | serine/threonine kinase 17b | XM_011512171.2 | 65.5% | 65.5% | 0_1ins384 |
| 6 | mouse | 98267 | Stk17b | serine/threonine kinase 17b... | NM_133810.3 | 87.3% | 91.3% | (many diffs) |
| 7 | mouse | 98267 | Stk17b | serine/threonine kinase 17b... | XM_006496359.3 | 58.2% | 60.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1188
- ORF length:
- 1116
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtcgagg aggagatttg attgccgaag tatttcaggc ctactaacta 121 caactcctca aattccaata aaaatggaaa actttaataa tttctatata cttacatcta 181 aagagctagg gagaggaaaa tttgctgtgg ttagacaatg tatatcaaaa tctactggcc 241 aagaatatgc tgcaaaattt ctaaaaaaga gaagaagagg acaggattgt cgagcagaaa 301 ttttacacga gattgctgtg cttgaattgg caaagtcttg tccccgtgtt attaatcttc 361 atgaggtcta tgaaaataca agtgaaatca ttttgatatt ggaatatgct gcaggtggag 421 aaattttcag cctgtgttta cctgagttgg ctgaaatggt ttctgaaaat gatgttatca 481 gactcattaa acaaatactt gaaggagttt attatctaca tcagaataac attgtacacc 541 ttgatttaaa gccacagaat atattactga gcagcatata ccctctcggg gacattaaaa 601 tagtagattt tggaatgtct cgaaaaatag ggcatgcgtg tgaacttcgg gaaatcatgg 661 gaacaccaga atatttagct ccagaaatcc tgaactatga tcccattacc acagcaacag 721 atatgtggaa tattggtata atagcatata tgttgttaac tcacacatca ccatttgtgg 781 gagaagataa tcaagaaaca tacctcaata tttctcaagt taatgtagat tattcggaag 841 aaactttttc atcagtttca cagctggcca cagactttat tcagagCCTT TTAGTAAAAA 901 ATCCAGAGAA AAGACCAACA GCAGAGATAT GCCTTTCTCA TTCTTGGCTA CAGCAGTGGG 961 ACTTTGAAAA CTTGTTTCAC CCTGAAGAAA CTTCCAGTTC CTCTCAAACT CAGGATCATT 1021 CTGTAAGGTC CTCTGAAGAC AAGACTTCTA AATCCTCCTG TAATGGAACC TGTGGTGATA 1081 GAGAAGACAA AGAGAATATC CCAGAGGATA GCAGCATGGT TTCCAAAAGA TTTCGTTTCG 1141 ATGACTCATT ACCCAATCCC CATGAACTTG TTTCAGATTT GCTCTGTTGA GACCCAGCTT 1201 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1261 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1321 CTTGTGGAAA GGACGATAGA CTCGTCGCGT CGTCTGCCGT ACGCGTTAAG TCgacaatca 1381 acctctggat tacaaaattt gtgaaagatt