Construct: ORF TRCN0000489414
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019495.1_s317c1
- DNA Barcode:
- ACATAGAATGGAGATTGAGGCCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- INSR (3643)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489414
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3643 | INSR | insulin receptor | NM_001079817.3 | 99.9% | 99.9% | 5C>G;3219C>T |
| 2 | human | 3643 | INSR | insulin receptor | XM_011527989.3 | 99.8% | 99.7% | 5C>G;2806_2807insTAG;3216C>T |
| 3 | human | 3643 | INSR | insulin receptor | NM_000208.4 | 99% | 99% | 5C>G;2230_2265del;3255C>T |
| 4 | human | 3643 | INSR | insulin receptor | XM_011527988.2 | 99% | 98.9% | (many diffs) |
| 5 | mouse | 16337 | Insr | insulin receptor | NM_010568.3 | 87% | 95.2% | (many diffs) |
| 6 | mouse | 16337 | Insr | insulin receptor | XM_006508701.1 | 86.9% | 95.1% | (many diffs) |
| 7 | mouse | 16337 | Insr | insulin receptor | NM_001330056.1 | 86.2% | 94.4% | (many diffs) |
| 8 | mouse | 16337 | Insr | insulin receptor | XM_006508700.1 | 86.1% | 94.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 105
- ORF end:
- 4215
- ORF length:
- 4110
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggccgccccc ttcaccggta ccgctcccgc agccatgggc accgggggcc 121 ggcggggggc ggcggccgcg ccgctgctgg tggcggtggc cgcgctgcta ctgggcgccg 181 cgggccacct gtaccccgga gaggtgtgtc ccggcatgga tatccggaac aacctcacta 241 ggttgcatga gctggagaat tgctctgtca tcgaaggaca cttgcagata ctcttgatgt 301 tcaaaacgag gcccgaagat ttccgagacc tcagtttccc caaactcatc atgatcactg 361 attacttgct gctcttccgg gtctatgggc tcgagagcct gaaggacctg ttccccaacc 421 tcacggtcat ccggggatca cgactgttct ttaactacgc gctggtcatc ttcgagatgg 481 ttcacctcaa ggaactcggc ctctacaacc tgatgaacat cacccggggt tctgtccgca 541 tcgagaagaa caatgagctc tgttacttgg ccactatcga ctggtcccgt atcctggatt 601 ccgtggagga taattacatc gtgttgaaca aagatgacaa cgaggagtgt ggagacatct 661 gtccgggtac cgcgaagggc aagaccaact gccccgccac cgtcatcaac gggcagtttg 721 tcgaacgatg ttggactcat agtcactgcc agaaagtttg cccgaccatc tgtaagtcac 781 acggctgcac cgccgaaggc ctctgttgcc acagcgagtg cctgggcaac tgttctcagc 841 ccgacgaccc caccaagtgc gtggcctgcc gcaacttcta cctggacggc aggtgtgtgg 901 agacctgccc gcccccgtac taccacttcc aggactggcg ctgtgtgaac ttcagcttct 961 gccaggacct gcaccacaaa tgcaagaact cgcggaggca gggctgccac cagtacgtca 1021 ttcacaacaa caagtgcatc cctgagtgtc cctccgggta cacgatgaat tccagcaact 1081 tgctgtgcac cccatgcctg ggtccctgtc ccaaggtgtg ccacctccta gaaggcgaga 1141 agaccatcga ctcggtgacg tctgcccagg agctccgagg atgcaccgtc atcaacggga 1201 gtctgatcat caacattcga ggaggcaaca atctggcagc tgagctagaa gccaacctcg 1261 gcctcattga agaaatttca gggtatctaa aaatccgccg atcctacgct ctggtgtcac 1321 tttccttctt ccggaagtta cgtctgattc gaggagagac cttggaaatt gggaactact 1381 ccttctatgc cttggacaac cagaacctaa ggcagctctg ggactggagc aaacacaacc 1441 tcaccatcac tcaggggaaa ctcttcttcc actataaccc caaactctgc ttgtcagaaa 1501 tccacaagat ggaagaagtt tcaggaacca aggggcgcca ggagagaaac gacattgccc 1561 tgaagaccaa tggggaccag gcatcctgtg aaaatgagtt acttaaattt tcttacattc 1621 ggacatcttt tgacaagatc ttgctgagat gggagccgta ctggcccccc gacttccgag 1681 acctcttggg gttcatgctg ttctacaaag aggcccctta tcagaatgtg acggagttcg 1741 acgggcagga tgcgtgtggt tccaacagtt ggacggtggt agacattgac ccacccctga 1801 ggtccaacga ccccaaatca cagaaccacc cagggtggct gatgcggggt ctcaagccct 1861 ggacccagta tgccatcttt gtgaagaccc tggtcacctt ttcggatgaa cgccggacct 1921 atggggccaa gagtgacatc atttatgtcc agacagatgc caccaacccc tctgtgcccc 1981 tggatccaat ctcagtgtct aactcatcat cccagattat tctgaagtgg aaaccaccct 2041 ccgaccccaa tggcaacatc acccactacc tggttttctg ggagaggcag gcggaagaca 2101 gtgagctgtt cgagctggat tattgcctca aagggctgaa gctgccctcg aggacctggt 2161 ctccaccatt cgagtctgaa gattctcaga agcacaacca gagtgagtat gaggattcgg 2221 ccggcgaatg ctgctcctgt ccaaagacag actctcagat cctgaaggag ctggaggagt 2281 cctcgtttag gaagacgttt gaggattacc tgcacaacgt ggttttcgtc cccaggccat 2341 ctcggaaacg caggtccctt ggcgatgttg ggaatgtgac ggtggccgtg cccacggtgg 2401 cagctttccc caacacttcc tcgaccagcg tgcccacgag tccggaggag cacaggcctt 2461 ttgagaaggt ggtgaacaag gagtcgctgg tcatctccgg cttgcgacac ttcacgggct 2521 atcgcatcga gctgcaggct tgcaaccagg acacccctga ggaacggtgc agtgtggcag 2581 cctacgtcag tgcgaggacc atgcctgaag ccaaggctga tgacattgtt ggccctgtga 2641 cgcatgaaat ctttgagaac aacgtcgtcc acttgatgtg gcaggagccg aaggagccca 2701 atggtctgat cgtgctgtat gaagtgagtt atcggcgata tggtgatgag gagctgcatc 2761 tctgcgtctc ccgcaagcac ttcgctctgg aacggggctg caggctgcgt gggctgtcac 2821 cggggaacta cagcgtgcga atccgggcca cctcccttgc gggcaacggc tcttggacgg 2881 aacccaccta tttctacgtg acagactatt tagacgtccc gtcaaatatt gcaaaaatta 2941 tcatcggccc cctcatcttt gtctttctct tcagtgttgt gattggaagt atttatctat 3001 tcctgagaaa gaggcagcca gatgggccgc tgggaccgct ttacgcttct tcaaaccctg 3061 agtatctcag tgccagtgat gtgtttccat gctctgtgta cgtgccggac gagtgggagg 3121 tgtctcgaga gaagatcacc ctccttcgag agctggggca gggctccttc ggcatggtgt 3181 atgagggcaa tgccagggac atcatcaagg gtgaggcaga gacccgcgtg gcggtgaaga 3241 cggtcaacga gtcagccagt ctccgagagc ggattgagtt cctcaatgag gcctcggtca 3301 tgaagggctt cacctgccat catgtggtgc gcctcctggg agtggtgtcc aagggccagc 3361 ccacgctggt ggtgatggag ctgatggctc acggagacct gaagagctac ctccgttctc 3421 tgcggccaga ggctgagaat aatcctggcc gccctccccc tacccttcaa gagatgattc 3481 agatggcggc agagattgct gacgggatgg cctacctgaa cgccaagaag tttgtgcatc 3541 gggacctggc agcgagaaac tgcatggtcg cccatgattt tactgtcaaa attggagact 3601 ttggaatgac cagagacatc tatgaaacgg attactaccg gaaagggggc aagggtctgc 3661 tccctgtacg gtggatggca ccggagtccc tgaaggatgg ggtcttcacc acttcttctg 3721 acatgtggtc ctttggcgtg gtcctttggg aaatcaccag cttggcagaa cagccttacc 3781 aaggcctgtc taatgaacag gtgttgaaat ttgtcatgga tggagggtat ctggatcaac 3841 ccgacaactg tccagagaga gtcactgacc TCATGCGCAT GTGCTGGCAA TTCAACCCCA 3901 AGATGAGGCC AACCTTCCTG GAGATTGTCA ACCTGCTCAA GGACGACCTG CACCCCAGCT 3961 TTCCAGAGGT GTCGTTCTTC CACAGCGAGG AGAACAAGGC TCCCGAGAGT GAGGAGCTGG 4021 AGATGGAGTT TGAGGACATG GAGAATGTGC CCCTGGACCG TTCCTCGCAC TGTCAGAGGG 4081 AGGAGGCGGG GGGCCGGGAT GGAGGGTCCT CGCTGGGTTT CAAGCGGAGC TACGAGGAAC 4141 ACATCCCTTA CACACACATG AACGGAGGCA AGAAAAACGG GCGGATTCTG ACCTTGCCTC 4201 GGTCCAATCC TTCCAAGGGT GGGCGCGCCG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 4261 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 4321 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACATA 4381 GAATGGAGAT TGAGGCCAAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 4441 tgaaagatt