Construct: ORF TRCN0000489425
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021805.1_s317c1
- DNA Barcode:
- CTGATGTACAGCTGTTCTTTAACA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SORT1 (6272)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489425
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6272 | SORT1 | sortilin 1 | NM_002959.7 | 99.9% | 100% | 594T>C;969A>C |
| 2 | human | 6272 | SORT1 | sortilin 1 | XM_005271100.2 | 99.7% | 99.7% | 594T>C;832_833insAAG;966A>C |
| 3 | human | 6272 | SORT1 | sortilin 1 | XM_005271101.3 | 83.5% | 83.6% | 0_1ins408;186T>C;561A>C |
| 4 | human | 6272 | SORT1 | sortilin 1 | XM_005271102.2 | 83.5% | 83.6% | 0_1ins408;186T>C;561A>C |
| 5 | human | 6272 | SORT1 | sortilin 1 | XM_006710812.2 | 83.5% | 83.6% | 0_1ins408;186T>C;561A>C |
| 6 | human | 6272 | SORT1 | sortilin 1 | NM_001205228.1 | 83.4% | 83.3% | (many diffs) |
| 7 | mouse | 20661 | Sort1 | sortilin 1 | NM_019972.3 | 87.1% | 90.8% | (many diffs) |
| 8 | mouse | 20661 | Sort1 | sortilin 1 | XM_006501224.3 | 86.9% | 90.6% | (many diffs) |
| 9 | mouse | 20661 | Sort1 | sortilin 1 | NM_001271599.1 | 84% | 87.3% | (many diffs) |
| 10 | mouse | 20661 | Sort1 | sortilin 1 | XM_006501223.3 | 83.9% | 87.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2562
- ORF length:
- 2493
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggagcggccc tggggagctg cggacggcct ctcgcgctgg ccccatggcc 121 tcggcctcct cctcctcctg cagctgctgc cgccgtcgac cctcagccag gaccggctgg 181 acgcgccgcc gccgcccgct gcgccgctgc cgcgctggtc tggccccatc ggggtgagct 241 gggggctgcg ggcggccgca gccgggggcg cgtttccccg cggcggccgt tggcgtcgca 301 gcgcgccggg cgaggacgag gagtgcggcc gggtccggga cttcgtcgcc aagctggcca 361 acaacacgca ccagcatgtg tttgatgatc tcagaggctc agtatccttg tcctgggttg 421 gagatagcac tggggtcatt ctagtcttga ctaccttcca tgtaccactg gtaattatga 481 cttttggaca gtccaagcta tatcgaagtg aggattatgg gaagaacttt aaggatatta 541 cagatctcat caataacacc tttattcgga ctgaatttgg catggctatt ggtcctgaga 601 actctggaaa ggtggtgtta acagcagagg tgtctggagg aagtcgtgga ggaagaatct 661 tcagatcatc agattttgcg aagaattttg tgcaaacaga tctccctttt catcctctca 721 ctcagatgat gtatagccct cagaattctg attatctttt agctctcagc actgaaaatg 781 gcctgtgggt gtccaagaat tttgggggaa aatgggaaga aatccacaaa gcagtatgtt 841 tggccaaatg gggatcagac aacaccatct tctttacaac ctatgcaaat ggctcctgca 901 aagctgacct tggggctctg gaattatgga gaacttcaga cttgggaaaa agcttcaaaa 961 ctattggtgt gaaaatctac tcatttggtc ttgggggacg tttccttttt gcctctgtga 1021 tggctgataa ggataccaca agaaggatcc acgtttcaac agatcaaggg gacacatgga 1081 gcatggccca gctcccctcc gtgggacagg aacagttcta ttctattctg gcagcaaatg 1141 atgacatggt attcatgcat gtagatgaac ctggagacac tgggtttggc acaatcttta 1201 cctcagatga tcgaggcatt gtctattcca agtctttgga ccgacatctc tacactacca 1261 caggcggaga gacggacttt accaacgtga cctccctccg cggcgtctac ataacaagcg 1321 tgctctccga agataattct atccagacca tgatcacttt tgaccaagga ggaaggtgga 1381 cgcacctgag gaagcctgaa aacagtgaat gtgatgctac agcaaaaaac aagaatgagt 1441 gcagccttca tattcatgct tcctacagca tctcccagaa actgaatgtt ccaatggccc 1501 cactctcaga gccgaatgcc gtaggcattg tcattgctca tggtagcgtg ggggatgcca 1561 tctcagtgat ggttccagat gtgtacatct cagatgatgg gggttactcc tggacaaaga 1621 tgctggaagg accccactat tacaccatcc tggattctgg aggcatcatt gtggccattg 1681 agcacagcag ccgtcctatc aatgtgatta agttctccac agacgaaggt caatgctggc 1741 aaacctacac gttcaccagg gaccccatct atttcactgg cctagcttca gaacctggag 1801 ctaggtccat gaatatcagc atttggggct tcacagaatc tttcctgacc agccagtggg 1861 tctcctacac cattgatttt aaagatatcc ttgaaaggaa ctgtgaagag aaggactata 1921 ccatatggct ggcacactcc acagaccctg aagattatga agatggctgc attttgggct 1981 acaaagaaca gtttctgcgg ctacgcaagt catccgtgtg tcagaatggt cgagactatg 2041 ttgtgaccaa gcagccctcc atctgcctct gttccctgga ggactttctc tgtgattttg 2101 gctactaccg tccagaaaat gactccaagt gtgtggaaca gccagaactg aagggccacg 2161 acctggagtt ttgtctgtac ggaagagaag aaCACCTAAC AACAAATGGG TACCGGAAAA 2221 TTCCAGGGGA CAAATGCCAG GGTGGGGTAA ATCCAGTTCG AGAAGTAAAA GACTTGAAAA 2281 AGAAATGCAC AAGCAACTTT TTGAGTCCGG AAAAACAGAA TTCCAAGTCA AATTCTGTTC 2341 CAATTATCCT GGCCATCGTG GGATTGATGC TGGTCACAGT CGTAGCAGGA GTGCTCATTG 2401 TGAAGAAATA TGTCTGTGGG GGAAGGTTCC TGGTGCATCG ATACTCTGTG CTGCAGCAGC 2461 ATGCAGAGGC CAATGGTGTG GATGGTGTGG ATGCTTTGGA CACAGCCTCC CACACTAATA 2521 AAAGTGGTTA TCATGATGAC TCAGATGAGG ACCTCTTGGA ATAGGACCCA GCTTTCTTGT 2581 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2641 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2701 GAAAGGACGA CTGATGTACA GCTGTTCTTT AACAACGCGT TAAGTCgaca atcaacctct 2761 ggattacaaa atttgtgaaa gatt