Construct: ORF TRCN0000489439

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020235.1_s317c1
DNA Barcode:
TGGGGACTCGCATTTTCGGGAGAG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
GALK2 (2585)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489439
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2585 GALK2 galactokinase 2 NM_002044.4 99.9% 100% 1221C>T
2 human 2585 GALK2 galactokinase 2 XM_005254279.4 98% 96.9% (many diffs)
3 human 2585 GALK2 galactokinase 2 XM_006720461.4 97.6% 97.1% (many diffs)
4 human 2585 GALK2 galactokinase 2 NM_001001556.3 96.3% 96.2% (many diffs)
5 human 2585 GALK2 galactokinase 2 NM_001289030.2 94.6% 94.7% 0_1ins72;1149C>T
6 human 2585 GALK2 galactokinase 2 NM_001289031.1 94.6% 94.7% 0_1ins72;1149C>T
7 human 2585 GALK2 galactokinase 2 XM_005254280.4 94.4% 93.3% (many diffs)
8 human 2585 GALK2 galactokinase 2 XM_006720462.4 92.8% 91.8% (many diffs)
9 human 2585 GALK2 galactokinase 2 XM_024449892.1 92.8% 91.8% (many diffs)
10 human 2585 GALK2 galactokinase 2 XM_006720463.4 90.9% 89.9% (many diffs)
11 human 2585 GALK2 galactokinase 2 XM_017022062.2 87.4% 87.5% 0_1ins72;432_433ins99;1050C>T
12 human 2585 GALK2 galactokinase 2 XM_005254284.4 87.4% 86.2% (many diffs)
13 human 2585 GALK2 galactokinase 2 XM_024449893.1 86.9% 86.3% (many diffs)
14 human 2585 GALK2 galactokinase 2 XM_024449894.1 85.7% 84.7% (many diffs)
15 human 2585 GALK2 galactokinase 2 XM_006720464.4 79.9% 78.9% (many diffs)
16 human 2585 GALK2 galactokinase 2 XM_017022063.2 76.4% 75.3% (many diffs)
17 human 2585 GALK2 galactokinase 2 XM_011521441.3 70.2% 69.3% (many diffs)
18 human 2585 GALK2 galactokinase 2 XM_017022064.2 70.2% 69.3% (many diffs)
19 human 2585 GALK2 galactokinase 2 NM_001352047.1 69.7% 69.3% (many diffs)
20 human 2585 GALK2 galactokinase 2 NM_001352048.1 69.7% 69.3% (many diffs)
21 human 2585 GALK2 galactokinase 2 XM_024449895.1 64.5% 64.6% 0_1ins387;117_118ins99;735C>T
22 human 2585 GALK2 galactokinase 2 XR_001751207.2 63.6% 1_91del;1058_1227del;1302_1303ins333
23 human 2585 GALK2 galactokinase 2 XR_001751204.2 33% (many diffs)
24 human 2585 GALK2 galactokinase 2 XR_001751202.2 32.3% (many diffs)
25 human 2585 GALK2 galactokinase 2 XR_001751205.2 31.9% (many diffs)
26 human 2585 GALK2 galactokinase 2 XR_002957633.1 31.3% (many diffs)
27 human 2585 GALK2 galactokinase 2 XR_001751206.2 31% (many diffs)
28 human 2585 GALK2 galactokinase 2 XR_001751208.2 30% (many diffs)
29 human 2585 GALK2 galactokinase 2 NR_147895.2 26.2% (many diffs)
30 mouse 69976 Galk2 galactokinase 2 NM_001291002.1 84.9% 88.8% (many diffs)
31 mouse 69976 Galk2 galactokinase 2 NM_175154.3 82% 85% (many diffs)
32 mouse 69976 Galk2 galactokinase 2 XM_006500153.3 80.6% 84% (many diffs)
33 mouse 69976 Galk2 galactokinase 2 XM_011239770.2 80.6% 84% (many diffs)
34 mouse 69976 Galk2 galactokinase 2 XM_006500154.1 69.8% 70% (many diffs)
35 mouse 69976 Galk2 galactokinase 2 XM_011239772.1 49.7% 51% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1446
ORF length:
1374
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggctaca gagagccctg ctacgcgtcg ggtccaggtg gcagaacatc
121ctaggttact gaagctaaag gagatgttta actccaagtt tggatctatt cccaagtttt
181atgttcgagc accaggaaga gtcaacataa taggagagca tatagattat tgtggatatt
241ctgttcttcc tatggctgta gaacaagatg tgctaatagc tgtagaacct gtgaaaacgt
301acgctctcca actggccaat acaaatccct tgtatccgga cttcagtact agtgctaata
361acatccagat tgataaaacc aagcctttgt ggcacaacta tttcttatgt ggacttaaag
421gaattcagga acactttggt cttagtaacc tgactggaat gaactgcctg gtagatggaa
481atatcccacc aagttctggc ctctccagct ccagtgcttt ggtctgttgt gctggcttgg
541tgacgctcac agtgctggga aggaatctat ccaaggtgga acttgcagaa atctgtgcca
601agagtgagcg ttacattggc actgaaggag gaggcatgga ccagtctata tcatttcttg
661cagaagaagg aactgccaag ttgatagaat ttagtcctct gagggcaacc gatgtaaaac
721tcccaagtgg agcagtgttt gtgattgcca acagttgtgt ggagatgaat aaggcagcaa
781cttcccattt caatatcagg gtgatggagt gtcggctggc tgcgaagctc ctggctaaat
841acaaaagctt gcaatgggac aaagtactga ggctggagga ggtgcaggct aaactaggga
901ttagtctaga agaaatgctg ttggtcacag aagatgccct tcatcctgaa ccctataacc
961ctgaggagat ctgcaggtgt ctgggaatta gcctggagga actccgaacc caaatcctga
1021gtccaaacac tcaagatgtg ctcaTCTTCA AACTCTATCA GCGGGCAAAG CATGTGTACA
1081GCGAGGCTGC GCGAGTGCTC CAGTTTAAGA AGATATGTGA AGAAGCACCT GAAAACATGG
1141TCCAGCTGCT GGGAGAGTTG ATGAACCAGA GCCACATGAG CTGCCGGGAC ATGTATGAGT
1201GCAGCTGCCC CGAGCTGGAT CAGCTGGTGG ACATCTGTCG GAAGTTTGGG GCTCAAGGGT
1261CACGACTTAC TGGAGCAGGA TGGGGAGGCT GTACAGTATC AATGGTACCT GCGGACAAGC
1321TGCCCAGCTT TCTAGCAAAT GTGCACAAAG CTTATTACCA GAGGAGTGAT GGAAGCTTAG
1381CACCGGAGAA GCAAAGTTTG TTTGCTACCA AACCTGGAGG TGGGGCTTTG GTTTTGCTTG
1441AGGCCTGAGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1501CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1561TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATGGGGA CTCGCATTTT CGGGAGAGAC
1621GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)

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