Construct: ORF TRCN0000489449
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019606.1_s317c1
- DNA Barcode:
- AGCAGTATTTACGCACTAGCTGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HTR2B (3357)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489449
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3357 | HTR2B | 5-hydroxytryptamine recepto... | NM_000867.5 | 99.7% | 99.5% | 640T>C;1430A>G;1442_1443delTA |
| 2 | human | 3357 | HTR2B | 5-hydroxytryptamine recepto... | XM_006712482.3 | 92.6% | 92.5% | (many diffs) |
| 3 | human | 3357 | HTR2B | 5-hydroxytryptamine recepto... | NM_001320758.2 | 90.9% | 90.8% | (many diffs) |
| 4 | human | 3357 | HTR2B | 5-hydroxytryptamine recepto... | XM_005246520.4 | 74.9% | 74.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1518
- ORF length:
- 1443
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggct ctctcttaca gagtgtctga acttcaaagc acaattcctg 121 agcacatttt gcagagcacc tttgttcacg ttatctcttc taactggtct ggattacaga 181 cagaatcaat accagaggaa atgaaacaga ttgttgagga acagggaaat aaactgcact 241 gggcagctct tctgatactc atggtgataa tacccacaat tggtggaaat acccttgtta 301 ttctggctgt ttcactggag aagaagctgc agtatgctac taattacttt ctaatgtcct 361 tggcggtggc tgatttgctg gttggattgt ttgtgatgcc aattgccctc ttgacaataa 421 tgtttgaggc tatgtggccc ctcccacttg ttctatgtcc tgcctggtta tttcttgacg 481 ttctcttttc aaccgcatcc atcatgcatc tctgtgccat ttcagtggat cgttacatag 541 ccatcaaaaa gccaatccag gccaatcaat ataactcacg ggctacagca ttcatcaaga 601 ttacagtggt gtggttaatt tcaataggca ttgccattcc agtccctatt aaagggatag 661 agactgatgt ggacaaccca aacaatatca cttgtgtgct gacaaaggaa cgtcttggcg 721 atttcatgct ctttggctca ctggctgcct tcttcacacc tcttgcaatt atgattgtca 781 cctactttct cactatccat gctttacaga agaaggctta cttagtcaaa aacaagccac 841 ctcaacgcct aacatggttg actgtgtcta cagttttcca aagggatgaa acaccttgct 901 cgtcaccgga aaaggtggca atgctggatg gttctcgaaa ggacaaggct ctgcccaact 961 caggtgatga aacacttatg cgaagaacat ccacaattgg gaaaaagtca gtgcagacca 1021 tttccaacga acagagagcc tcaaaggtcc tagggattgt gtttttcctc tttttgctta 1081 tgtggtgtcc cttctttatt acaaatataa ctttagtttt atgtgattcc tgtaaCCAAA 1141 CTACTCTCCA AATGCTCCTG GAGATATTTG TGTGGATAGG CTATGTTTCC TCAGGAGTGA 1201 ATCCTTTGGT CTACACCCTC TTCAATAAGA CATTTCGGGA TGCATTTGGC CGATATATCA 1261 CCTGCAATTA CCGGGCCACA AAGTCAGTAA AAACTCTCAG AAAACGCTCC AGTAAGATCT 1321 ACTTCCGGAA TCCAATGGCA GAGAACTCTA AGTTTTTCAA GAAACATGGA ATTCGAAATG 1381 GGATTAACCC TGCCATGTAC CAGAGTCCAA TGAGGCTCCG AAGTTCAACC ATTCAGTCTT 1441 CATCAATCAT TCTACTAGAT ACGCTTCTCC TCACTGAAAA TGAAGGTGAC AAAACTGAAG 1501 AGCGAGTTAG TTATGTAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1561 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1621 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAGCAGTA TTTACGCACT 1681 AGCTGCAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt