Construct: ORF TRCN0000489469
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020306.1_s317c1
- DNA Barcode:
- ACCTGGAAGACTAAACTACCCCCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MLH1 (4292)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489469
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4292 | MLH1 | mutL homolog 1 | NM_000249.3 | 99.8% | 99.8% | 2268_2269insTTG |
| 2 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354628.1 | 95.7% | 95.7% | 1892_1893ins93;2175_2176insTTG |
| 3 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354629.1 | 95.5% | 95.5% | 206_207ins99;2169_2170insTTG |
| 4 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354630.1 | 92.6% | 92.6% | 1730_1731ins165;2103_2104insTTG |
| 5 | human | 4292 | MLH1 | mutL homolog 1 | NM_001258271.1 | 90.7% | 90.7% | 1894_1895ins207;2061_2062insTTG |
| 6 | human | 4292 | MLH1 | mutL homolog 1 | XM_005265161.2 | 90.7% | 90.7% | 676_677ins207;2061_2062insTTG |
| 7 | human | 4292 | MLH1 | mutL homolog 1 | NM_001167617.2 | 86.9% | 86.5% | 0_1ins289;9_10insGAGGT;1974_1975insTTG |
| 8 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354620.1 | 86.9% | 86.5% | 0_1ins289;9_10insGAGGT;1974_1975insTTG |
| 9 | human | 4292 | MLH1 | mutL homolog 1 | NM_001167618.2 | 68% | 68% | 0_1ins723;1545_1546insTTG |
| 10 | human | 4292 | MLH1 | mutL homolog 1 | NM_001167619.2 | 68% | 68% | 0_1ins723;1545_1546insTTG |
| 11 | human | 4292 | MLH1 | mutL homolog 1 | NM_001258273.1 | 68% | 68% | 0_1ins723;1545_1546insTTG |
| 12 | human | 4292 | MLH1 | mutL homolog 1 | NM_001258274.2 | 68% | 68% | 0_1ins723;1545_1546insTTG |
| 13 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354615.1 | 68% | 68% | 0_1ins723;1545_1546insTTG |
| 14 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354616.1 | 68% | 68% | 0_1ins723;1545_1546insTTG |
| 15 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354617.1 | 68% | 68% | 0_1ins723;1545_1546insTTG |
| 16 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354618.1 | 68% | 68% | 0_1ins723;1545_1546insTTG |
| 17 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354619.1 | 68% | 68% | 0_1ins723;1545_1546insTTG |
| 18 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354621.1 | 54.8% | 54.8% | 0_1ins1023;1245_1246insTTG |
| 19 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354622.1 | 54.8% | 54.8% | 0_1ins1023;1245_1246insTTG |
| 20 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354623.1 | 54.8% | 54.8% | 0_1ins1023;1245_1246insTTG |
| 21 | human | 4292 | MLH1 | mutL homolog 1 | XM_017006450.2 | 54.8% | 54.8% | 0_1ins1023;1245_1246insTTG |
| 22 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354624.1 | 52.5% | 52.5% | 0_1ins1074;1194_1195insTTG |
| 23 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354625.1 | 52.5% | 52.5% | 0_1ins1074;1194_1195insTTG |
| 24 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354626.1 | 52.5% | 52.5% | 0_1ins1074;1194_1195insTTG |
| 25 | human | 4292 | MLH1 | mutL homolog 1 | NM_001354627.1 | 52.5% | 52.5% | 0_1ins1074;1194_1195insTTG |
| 26 | mouse | 17350 | Mlh1 | mutL homolog 1 | NM_026810.2 | 86.6% | 88.3% | (many diffs) |
| 27 | mouse | 17350 | Mlh1 | mutL homolog 1 | XM_011242935.2 | 79.8% | 79.6% | (many diffs) |
| 28 | mouse | 17350 | Mlh1 | mutL homolog 1 | XR_379848.2 | 65.1% | (many diffs) | |
| 29 | mouse | 17350 | Mlh1 | mutL homolog 1 | XR_001778805.1 | 63.9% | (many diffs) | |
| 30 | mouse | 17350 | Mlh1 | mutL homolog 1 | XR_379849.3 | 59.5% | (many diffs) | |
| 31 | mouse | 17350 | Mlh1 | mutL homolog 1 | XM_006511946.3 | 58.2% | 53.9% | (many diffs) |
| 32 | mouse | 17350 | Mlh1 | mutL homolog 1 | NM_001324522.1 | 58% | 58.2% | (many diffs) |
| 33 | mouse | 17350 | Mlh1 | mutL homolog 1 | XM_006511948.3 | 58% | 58.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2343
- ORF length:
- 2271
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtcgttc gtggcagggg ttattcggcg gctggacgag acagtggtga 121 accgcatcgc ggcgggggaa gttatccagc ggccagctaa tgctatcaaa gagatgattg 181 agaactgttt agatgcaaaa tccacaagta ttcaagtgat tgttaaagag ggaggcctga 241 agttgattca gatccaagac aatggcaccg ggatcaggaa agaagatctg gatattgtat 301 gtgaaaggtt cactactagt aaactgcagt cctttgagga tttagccagt atttctacct 361 atggctttcg aggtgaggct ttggccagca taagccatgt ggctcatgtt actattacaa 421 cgaaaacagc tgatggaaag tgtgcataca gagcaagtta ctcagatgga aaactgaaag 481 cccctcctaa accatgtgct ggcaatcaag ggacccagat cacggtggag gacctttttt 541 acaacatagc cacgaggaga aaagctttaa aaaatccaag tgaagaatat gggaaaattt 601 tggaagttgt tggcaggtat tcagtacaca atgcaggcat tagtttctca gttaaaaaac 661 aaggagagac agtagctgat gttaggacac tacccaatgc ctcaaccgtg gacaatattc 721 gctccatctt tggaaatgct gttagtcgag aactgataga aattggatgt gaggataaaa 781 ccctagcctt caaaatgaat ggttacatat ccaatgcaaa ctactcagtg aagaagtgca 841 tcttcttact cttcatcaac catcgtctgg tagaatcaac ttccttgaga aaagccatag 901 aaacagtgta tgcagcctat ttgcccaaaa acacacaccc attcctgtac ctcagtttag 961 aaatcagtcc ccagaatgtg gatgttaatg tgcaccccac aaagcatgaa gttcacttcc 1021 tgcacgagga gagcatcctg gagcgggtgc agcagcacat cgagagcaag ctcctgggct 1081 ccaattcctc caggatgtac ttcacccaga ctttgctacc aggacttgct ggcccctctg 1141 gggagatggt taaatccaca acaagtctga cctcgtcttc tacttctgga agtagtgata 1201 aggtctatgc ccaccagatg gttcgtacag attcccggga acagaagctt gatgcatttc 1261 tgcagcctct gagcaaaccc ctgtccagtc agccccaggc cattgtcaca gaggataaga 1321 cagatatttc tagtggcagg gctaggcagc aagatgagga gatgcttgaa ctcccagccc 1381 ctgctgaagt ggctgccaaa aatcagagct tggaggggga tacaacaaag gggacttcag 1441 aaatgtcaga gaagagagga cctacttcca gcaaccccag aaagagacat cgggaagatt 1501 ctgatgtgga aatggtggaa gatgattccc gaaaggaaat gactgcagct tgtacccccc 1561 ggagaaggat cattaacctc actagtgttt tgagtctcca ggaagaaatt aatgagcagg 1621 gacatgaggt tctccgggag atgttgcata accactcctt cgtgggctgt gtgaatcctc 1681 agtgggcctt ggcacagcat caaaccaagt tataccttct caacaccacc aagcttagtg 1741 aagaactgtt ctaccagata ctcatttatg attttgccaa ttttggtgtt ctcaggttat 1801 cggagccagc accgctcttt gaccttgcca tgcttgcctt agatagtcca gagagtggct 1861 ggacagagga agatggtccc aaagaaggac ttgctgaata cattgttgag tttctgaaga 1921 agaaggctga gatgcttgca gactatttct ctttggaaat tgatgaggaa gggaacctga 1981 ttggattacc ccttcTGATT GACAACTATG TGCCCCCTTT GGAGGGACTG CCTATCTTCA 2041 TTCTTCGACT AGCCACTGAG GTGAATTGGG ACGAAGAAAA GGAATGTTTT GAAAGCCTCA 2101 GTAAAGAATG CGCTATGTTC TATTCCATCC GGAAGCAGTA CATATCTGAG GAGTCGACCC 2161 TCTCAGGCCA GCAGAGTGAA GTGCCTGGCT CCATTCCAAA CTCCTGGAAG TGGACTGTGG 2221 AACACATTGT CTATAAAGCC TTGCGCTCAC ACATTCTGCC TCCTAAACAT TTCACAGAAG 2281 ATGGAAATAT CCTGCAGCTT GCTAACCTGC CTGATCTATA CAAAGTCTTT GAGAGGTGTT 2341 TGTGAGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2401 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2461 CTTGGCTTTA TATATCTTGT GGAAAGGACG AACCTGGAAG ACTAAACTAC CCCCCACGCG 2521 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt