Construct: ORF TRCN0000489479

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019665.1_s317c1
DNA Barcode:
CGCGGCATTACAACGAGCCGGACG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ADCY2 (108)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489479
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 108 ADCY2 adenylate cyclase 2 NM_020546.3 99.5% 99.4% 1_15del;3273_3274insG
2 human 108 ADCY2 adenylate cyclase 2 XM_011513942.2 95.2% 95.2% 1_15del;1954_1955ins138;3135_3136insG
3 human 108 ADCY2 adenylate cyclase 2 XR_427657.2 81.9% 1_29del;2481_2482insAGAATGAATATTACTGT;3271_3940delinsG
4 human 108 ADCY2 adenylate cyclase 2 XR_001741974.2 80.2% 1_29del;1885_1886ins85;3203_3869delinsG
5 human 108 ADCY2 adenylate cyclase 2 XR_001741973.1 70% 1_29del;2896_3591del;3984_4653delinsG
6 mouse 210044 Adcy2 adenylate cyclase 2 NM_153534.2 87.7% 94.7% (many diffs)
7 mouse 210044 Adcy2 adenylate cyclase 2 XR_001780768.2 67.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
75
ORF end:
3336
ORF length:
3261
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcgc caccatgcgg cgccgccgct acctgcggga ccgctccgag gaggcggcgg
121gcggcggaga cgggctgccg cggtcccggg actggctcta cgagtcctac tactgcatga
181gccagcagca cccgctcatc gtcttcctgc tgctcatcgt catgggctcc tgcctcgccc
241tgctcgccgt cttcttcgcg ctcgggctgg aagttgaaga ccatgtggcg tttctaataa
301cagttccaac tgccctggcg attttctttg cgatatttat cctggtctgc atcgagtctg
361tgtttaagaa gctgctgcgc ctcttctcgt tggtgatatg gatatgcctt gttgccatgg
421gatacctgtt catgtgtttt ggaggcaccg tctctccctg ggaccaggta tcgttcttcc
481tcttcatcat cttcgtggtg tacaccatgc tgcccttcaa catgcgagac gccatcattg
541ccagcgtcct cacctcctcc tcccacacca tcgtgcttag cgtctgcctg tctgcaacac
601cgggaggcaa ggagcacctg gtctggcaga tcctggccaa tgtgatcatt ttcatctgtg
661ggaacctggc gggagcctac cataagcacc tcatggaact cgctcttcag caaacatatc
721aggacacctg taattgcatc aagtcgcgga tcaagttgga atttgaaaaa cgtcaacagg
781agcggcttct gctctccctg ctgccggccc acatcgccat ggagatgaaa gcggagatca
841tccagaggct gcagggcccc aaggcgggcc agatggagaa cacaaataac ttccacaacc
901tgtatgtgaa gcggcataca aacgtgagca tcttatacgc tgacatcgtt ggctttaccc
961ggctggcaag tgactgctcc ccgggagaac tagtccacat gctgaatgag ctctttggaa
1021agtttgatca aattgcaaag gagaatgaat gcatgagaat taaaatttta ggagactgct
1081actactgtgt atctggactc cctatatctc tccctaacca tgccaagaac tgtgtgaaaa
1141tggggctgga catgtgtgaa gccataaaga aagtgaggga tgctactgga gttgatatca
1201acatgcgcgt gggcgtgcat tctgggaatg tcctgtgtgg cgtgattggt ctgcagaagt
1261ggcaatatga tgtgtggtca catgatgtga ccttggccaa ccacatggaa gctggagggg
1321tccctggacg tgttcacatt tcttctgtca ccctggagca cttgaatggc gcttataaag
1381tggaggaggg agatggtgac attagggacc catatttaaa acagcacctg gtgaaaacct
1441actttgtgat caaccccaag ggagaacgac ggagccccca gcatctcttc agacctcgcc
1501acacccttga tggagccaaa atgagggcct cggtccgcat gacccggtac ttggagtcct
1561ggggggcagc caagcccttt gcacacctac atcacaggga cagcatgacc acagagaacg
1621gcaagatcag caccacggat gtacccatgg gtcagcataa ttttcaaaat cgcaccttaa
1681gaaccaagtc acaaaagaag agatttgaag aagaattgaa tgaaaggatg attcaagcaa
1741ttgatgggat taatgcacag aagcaatggc tcaagtctga agacattcag agaatctcac
1801tgcttttcta taacaaagta ctagaaaaag agtaccgggc cacggcactg ccagcgttca
1861agtattatgt gacttgtgcc tgtctcatat tcttctgcat cttcattgtg cagattctcg
1921tgctgccaaa aacgtccgtc ctgggcatct cctttggggc tgcgtttctc ttgctggcct
1981tcatcctctt cgtctgcttt gctggacagc ttctgcaatg cagcaaaaaa gcctctcccc
2041tgctcatgtg gcttttgaag tcctcgggca tcattgccaa ccgcccctgg ccacggatct
2101ctctcacgat catcaccaca gccatcatat taatgatggc cgtgttcaac atgtttttcc
2161tgagtgactc agaggaaaca atccctccaa ctgccaacac aacaaacaca agcttttcag
2221cctcaaataa tcaggtggcg attctgcgtg cgcagaattt atttttcctc ccgtacttta
2281tctacagctg cattctggga ctgatatcct gttccgtgtt cctgcgggta aactatgagc
2341tgaagatgtt gatcatgatg gtggccttgg tgggctacaa caccatccta ctccacaccc
2401acgcccacgt cctgggcgac tacagccagg tcttatttga gagaccaggc atttggaaag
2461acctgaagac catgggctct gtgtctctct ctatattctt catcacactg cttgttctgg
2521gtagacagaa tgaatattac tgtaggttag acttcttatg gaagaacaaa ttcaaaaaag
2581agcgggagga gatagagacc atggagaacc tgaaccgcgt gctgctggag aacgtgcttc
2641ccgcgcacgt ggctgagcac ttcctggcca ggagcctgaa gaatgaggag ctataccacc
2701agtcctatga ctgcgtctgc gtcatgtttg cctccattcc ggatttcaaa gaattttata
2761cagaatccga cgtgaacaag gagggcttgg aatgccttcg gctcctgaac gagatcatcg
2821ctgactttga tgatcttctt tccaagccaa aattcagtgg agttgaaaag attaagacca
2881ttggcagcac atacatggca gcaacaggtc tgagcgctgt gcccagccag gagcactccc
2941aggagcccga gcggcagtac atgcacattg gcaccatggt ggagtttgct tttgcccTGG
3001TAGGGAAGCT GGATGCCATC AACAAGCACT CCTTCAACGA CTTCAAATTG CGAGTGGGTA
3061TTAACCATGG ACCTGTGATA GCTGGTGTGA TTGGAGCTCA GAAGCCACAA TATGATATCT
3121GGGGCAACAC TGTCAATGTG GCCAGTAGGA TGGACAGCAC CGGAGTCCTG GACAAAATAC
3181AGGTTACCGA GGAGACGAGC CTCGTCCTGC AGACCCTCGG ATACACGTGC ACCTGTCGAG
3241GAATAATCAA CGTGAAAGGA AAGGGGGACC TGAAGACGTA CTTTGTAAAC ACAGAAATGT
3301CAAGGTCCCT TTCCCAGAGC AACGTGGCAT CCGACCCAGC TTTCTTGTAC AAAGTGGTTG
3361ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
3421GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACG
3481CGGCATTACA ACGAGCCGGA CGACGCGTTA AGTCgacaat caacctctgg attacaaaat
3541ttgtgaaaga tt

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.