Construct: ORF TRCN0000489479
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019665.1_s317c1
- DNA Barcode:
- CGCGGCATTACAACGAGCCGGACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADCY2 (108)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489479
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 108 | ADCY2 | adenylate cyclase 2 | NM_020546.3 | 99.5% | 99.4% | 1_15del;3273_3274insG |
| 2 | human | 108 | ADCY2 | adenylate cyclase 2 | XM_011513942.2 | 95.2% | 95.2% | 1_15del;1954_1955ins138;3135_3136insG |
| 3 | human | 108 | ADCY2 | adenylate cyclase 2 | XR_427657.2 | 81.9% | 1_29del;2481_2482insAGAATGAATATTACTGT;3271_3940delinsG | |
| 4 | human | 108 | ADCY2 | adenylate cyclase 2 | XR_001741974.2 | 80.2% | 1_29del;1885_1886ins85;3203_3869delinsG | |
| 5 | human | 108 | ADCY2 | adenylate cyclase 2 | XR_001741973.1 | 70% | 1_29del;2896_3591del;3984_4653delinsG | |
| 6 | mouse | 210044 | Adcy2 | adenylate cyclase 2 | NM_153534.2 | 87.7% | 94.7% | (many diffs) |
| 7 | mouse | 210044 | Adcy2 | adenylate cyclase 2 | XR_001780768.2 | 67.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 3336
- ORF length:
- 3261
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgcgg cgccgccgct acctgcggga ccgctccgag gaggcggcgg 121 gcggcggaga cgggctgccg cggtcccggg actggctcta cgagtcctac tactgcatga 181 gccagcagca cccgctcatc gtcttcctgc tgctcatcgt catgggctcc tgcctcgccc 241 tgctcgccgt cttcttcgcg ctcgggctgg aagttgaaga ccatgtggcg tttctaataa 301 cagttccaac tgccctggcg attttctttg cgatatttat cctggtctgc atcgagtctg 361 tgtttaagaa gctgctgcgc ctcttctcgt tggtgatatg gatatgcctt gttgccatgg 421 gatacctgtt catgtgtttt ggaggcaccg tctctccctg ggaccaggta tcgttcttcc 481 tcttcatcat cttcgtggtg tacaccatgc tgcccttcaa catgcgagac gccatcattg 541 ccagcgtcct cacctcctcc tcccacacca tcgtgcttag cgtctgcctg tctgcaacac 601 cgggaggcaa ggagcacctg gtctggcaga tcctggccaa tgtgatcatt ttcatctgtg 661 ggaacctggc gggagcctac cataagcacc tcatggaact cgctcttcag caaacatatc 721 aggacacctg taattgcatc aagtcgcgga tcaagttgga atttgaaaaa cgtcaacagg 781 agcggcttct gctctccctg ctgccggccc acatcgccat ggagatgaaa gcggagatca 841 tccagaggct gcagggcccc aaggcgggcc agatggagaa cacaaataac ttccacaacc 901 tgtatgtgaa gcggcataca aacgtgagca tcttatacgc tgacatcgtt ggctttaccc 961 ggctggcaag tgactgctcc ccgggagaac tagtccacat gctgaatgag ctctttggaa 1021 agtttgatca aattgcaaag gagaatgaat gcatgagaat taaaatttta ggagactgct 1081 actactgtgt atctggactc cctatatctc tccctaacca tgccaagaac tgtgtgaaaa 1141 tggggctgga catgtgtgaa gccataaaga aagtgaggga tgctactgga gttgatatca 1201 acatgcgcgt gggcgtgcat tctgggaatg tcctgtgtgg cgtgattggt ctgcagaagt 1261 ggcaatatga tgtgtggtca catgatgtga ccttggccaa ccacatggaa gctggagggg 1321 tccctggacg tgttcacatt tcttctgtca ccctggagca cttgaatggc gcttataaag 1381 tggaggaggg agatggtgac attagggacc catatttaaa acagcacctg gtgaaaacct 1441 actttgtgat caaccccaag ggagaacgac ggagccccca gcatctcttc agacctcgcc 1501 acacccttga tggagccaaa atgagggcct cggtccgcat gacccggtac ttggagtcct 1561 ggggggcagc caagcccttt gcacacctac atcacaggga cagcatgacc acagagaacg 1621 gcaagatcag caccacggat gtacccatgg gtcagcataa ttttcaaaat cgcaccttaa 1681 gaaccaagtc acaaaagaag agatttgaag aagaattgaa tgaaaggatg attcaagcaa 1741 ttgatgggat taatgcacag aagcaatggc tcaagtctga agacattcag agaatctcac 1801 tgcttttcta taacaaagta ctagaaaaag agtaccgggc cacggcactg ccagcgttca 1861 agtattatgt gacttgtgcc tgtctcatat tcttctgcat cttcattgtg cagattctcg 1921 tgctgccaaa aacgtccgtc ctgggcatct cctttggggc tgcgtttctc ttgctggcct 1981 tcatcctctt cgtctgcttt gctggacagc ttctgcaatg cagcaaaaaa gcctctcccc 2041 tgctcatgtg gcttttgaag tcctcgggca tcattgccaa ccgcccctgg ccacggatct 2101 ctctcacgat catcaccaca gccatcatat taatgatggc cgtgttcaac atgtttttcc 2161 tgagtgactc agaggaaaca atccctccaa ctgccaacac aacaaacaca agcttttcag 2221 cctcaaataa tcaggtggcg attctgcgtg cgcagaattt atttttcctc ccgtacttta 2281 tctacagctg cattctggga ctgatatcct gttccgtgtt cctgcgggta aactatgagc 2341 tgaagatgtt gatcatgatg gtggccttgg tgggctacaa caccatccta ctccacaccc 2401 acgcccacgt cctgggcgac tacagccagg tcttatttga gagaccaggc atttggaaag 2461 acctgaagac catgggctct gtgtctctct ctatattctt catcacactg cttgttctgg 2521 gtagacagaa tgaatattac tgtaggttag acttcttatg gaagaacaaa ttcaaaaaag 2581 agcgggagga gatagagacc atggagaacc tgaaccgcgt gctgctggag aacgtgcttc 2641 ccgcgcacgt ggctgagcac ttcctggcca ggagcctgaa gaatgaggag ctataccacc 2701 agtcctatga ctgcgtctgc gtcatgtttg cctccattcc ggatttcaaa gaattttata 2761 cagaatccga cgtgaacaag gagggcttgg aatgccttcg gctcctgaac gagatcatcg 2821 ctgactttga tgatcttctt tccaagccaa aattcagtgg agttgaaaag attaagacca 2881 ttggcagcac atacatggca gcaacaggtc tgagcgctgt gcccagccag gagcactccc 2941 aggagcccga gcggcagtac atgcacattg gcaccatggt ggagtttgct tttgcccTGG 3001 TAGGGAAGCT GGATGCCATC AACAAGCACT CCTTCAACGA CTTCAAATTG CGAGTGGGTA 3061 TTAACCATGG ACCTGTGATA GCTGGTGTGA TTGGAGCTCA GAAGCCACAA TATGATATCT 3121 GGGGCAACAC TGTCAATGTG GCCAGTAGGA TGGACAGCAC CGGAGTCCTG GACAAAATAC 3181 AGGTTACCGA GGAGACGAGC CTCGTCCTGC AGACCCTCGG ATACACGTGC ACCTGTCGAG 3241 GAATAATCAA CGTGAAAGGA AAGGGGGACC TGAAGACGTA CTTTGTAAAC ACAGAAATGT 3301 CAAGGTCCCT TTCCCAGAGC AACGTGGCAT CCGACCCAGC TTTCTTGTAC AAAGTGGTTG 3361 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 3421 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACG 3481 CGGCATTACA ACGAGCCGGA CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 3541 ttgtgaaaga tt