Construct: ORF TRCN0000489506
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019538.1_s317c1
- DNA Barcode:
- GAAACAGACTACTTCGTCAAGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADGRB3 (577)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489506
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 577 | ADGRB3 | adhesion G protein-coupled ... | NM_001704.3 | 99.9% | 99.8% | 1508A>G;4008G>A;4566_4567insG |
| 2 | mouse | 210933 | Adgrb3 | adhesion G protein-coupled ... | NM_175642.4 | 92.3% | 98.3% | (many diffs) |
| 3 | mouse | 210933 | Adgrb3 | adhesion G protein-coupled ... | XM_006495817.4 | 90.2% | 96% | (many diffs) |
| 4 | mouse | 210933 | Adgrb3 | adhesion G protein-coupled ... | XM_017319555.2 | 71.6% | 76.2% | (many diffs) |
| 5 | mouse | 210933 | Adgrb3 | adhesion G protein-coupled ... | XM_006495818.4 | 56.5% | 60.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 4644
- ORF length:
- 4569
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgaag gctgttcgta acctgctgat ttatatattt tccacctatc 121 tcctggttat gtttggattt aatgctgccc aagacttctg gtgttcaact ttggtgaagg 181 gagtcattta tggatcgtat tctgtaagtg aaatgtttcc taaaaacttt acaaactgca 241 cttggacgct ggaaaatcca gatccaacca aatatagcat ttacctgaaa ttttccaaaa 301 aggaccttag ctgctctaac ttttcactcc tggcttatca gtttgatcat ttttcccatg 361 aaaaaataaa ggatctttta agaaagaatc attctataat gcaactctgc aattccaaga 421 atgctttcgt ttttctacag tatgataaaa attttattca aatacgtcga gtatttccaa 481 ctaatttccc aggattacag aaaaaagggg aagaagatca gaaatctttt tttgagtttt 541 tggtattgaa caaggtcagc ccaagccagt ttggttgcca tgtattatgt acttggttgg 601 agagctgctt aaaatcagaa aatgggagaa cagaatcatg tgggatcatg tatacaaaat 661 gcacctgccc tcagcatttg ggagagtggg ggatcgacga ccagtcgctg attttgttaa 721 ataacgtggt gttacccctg aatgagcaga cagagggctg cctgacccag gagctgcaaa 781 ccacccaagt ctgcaatctt accagggagg ccaagcgacc acccaaagaa gaatttggaa 841 tgatgggaga tcatacaatt aaaagtcagc gacctcgatc tgttcatgaa aaaagggtcc 901 ctcaggaaca agctgatgct gctaaattta tggcacaaac tggtgaatct ggtgtggaag 961 agtggtccca gtggagcaca tgttcggtta cttgtggtca agggtcgcag gtgcgaacca 1021 gaacttgtgt atcaccttac gggacacact gcagcggccc attaagagaa tcaagggttt 1081 gcaataacac tgccctctgt ccagtacacg gagtatggga ggaatggtca ccatggagtt 1141 tatgttcatt tacatgtggt cgaggccaaa gaacaagaac aaggtcatgc acacctcctc 1201 agtatggagg aaggccgtgt gaaggacctg aaacacatca taagccttgt aatattgctc 1261 tttgcccagt tgatggacag tggcaagagt ggagttcgtg gagccagtgc tcagtaacgt 1321 gctcgaatgg gactcagcag agaagccggc agtgcactgc agctgcccat ggaggctccg 1381 aatgcagagg gccatgggca gaaagcagag agtgctataa ccctgaatgt acagccaatg 1441 gtcaatggaa tcagtggggt cattggagtg gttgttccaa gtcctgtgat ggcggctggg 1501 aaaggcgaat aaggacctgt cagggtgcag tgataacagg gcagcaatgt gaaggaacgg 1561 gcgaagaagt gagaagatgc agtgagcagc gatgccctgc accttatgaa atatgccctg 1621 aggattatct gatgtcgatg gtgtggaaaa gaactccagc aggcgacttg gcattcaatc 1681 aatgtcccct gaatgccaca ggcaccacta gcagacgctg ctctctcagt cttcatggag 1741 tggccttctg ggaacagccg agctttgcaa gatgcatatc aaatgagtac agacacttgc 1801 agcattcaat taaagagcac cttgctaagg ggcagcgaat gctggcaggt gatggaatgt 1861 cccaggtgac caagacactg ttggatttaa ctcagagaaa aaatttctat gcaggcgatc 1921 ttctgatgtc tgtggagatc ctgagaaatg tgacagacac atttaaaagg gcaagttaca 1981 tccctgcatc tgatggtgtc cagaacttct ttcaaatagt tagcaacctt ctagatgaag 2041 aaaacaagga aaaatgggaa gatgcacaac agatttatcc agggtcaata gagttaatgc 2101 aggtgattga agattttata cacattgttg gaatggggat gatggacttt cagaattcat 2161 acttaatgac tggaaatgta gtggctagta ttcagaagct tcctgcagcc tctgttctaa 2221 cagacatcaa ctttccaatg aaaggacgga agggaatggt tgactgggca agaaactcag 2281 aagatagggt agtaattcca aaaagcattt tcactccggt gtcatcaaaa gaattagatg 2341 aatcatctgt atttgttctt ggcgcagtcc tatacaaaaa cttagatcta attttgccca 2401 ctttgagaaa ttatactgtc attaattcca aaatcatcgt ggtcacaata aggcctgaac 2461 ccaaaacaac cgattcgttt ctggagatag aactagctca tttggctaat ggtactttga 2521 atccctattg tgtattgtgg gatgactcca aaacgaacga gtctttggga acgtggtcca 2581 cccagggatg taaaactgtg cttaccgatg catcccatac gaaatgctta tgtgatcgtc 2641 tctctacctt cgccattttg gctcagcaac ctagagaaat aatcatggaa tcctctggca 2701 caccttcagt taccctaata gtaggcagtg gtctttcttg cttggccttg attaccctag 2761 cagttgtcta tgcagcatta tggaggtaca tacgctctga gagatccata atactaatta 2821 acttctgcct gtctatcatc tcatccaata tcctcatact ggttggacag actcagacac 2881 ataataagag tatctgcaca accaccactg catttttgca ctttttcttc ctggcttcat 2941 tctgttgggt tttgactgag gcgtggcaat catatatggc tgtaactgga aaaattagga 3001 cacggcttat aagaaaacgc tttttgtgcc ttggatgggg tttaccagca ttagtagtgg 3061 ccacatcagt aggcttcacc agaacaaaag gatatggcac tgatcactac tgctggctct 3121 ctcttgaagg aggactactc tatgcttttg tgggacctgc agccgctgtt gtcctggtca 3181 acatggtgat tggcattttg gtatttaata aacttgtttc cagagatgga atcctagata 3241 aaaagctcaa acacagagcc ggtcagatga gtgagcctca tagcggtttg acgctcaaat 3301 gtgccaagtg tggagtagtt tcaacaacag ctttgtcagc caccaccgcc agtaacgcca 3361 tggcgtctct ttggagctcc tgtgtggtgt tgccccttct ggctttgacg tggatgtctg 3421 cggttctggc catgacagat aaacgctcca tattgtttca aatacttttt gctgtgtttg 3481 attcattgca aggctttgtt atagtcatgg tccactgcat tcttcggaga gaggttcagg 3541 atgcatttag atgccgattg agaaactgtc aggatcccat caatgcagat tcttcgagtt 3601 cgtttcctaa tgggcatgct caaatcatga cagactttga aaaggatgta gacattgcct 3661 gtcgatcagt tcttcataag gatattggtc cttgccgagc agccacaata acaggaacac 3721 tttctaggat ttctctaaat gatgatgaag aagaaaaggg aacaaaccct gaagggctaa 3781 gctattcaac attgcctgga aatgtcattt ccaaagtcat catccagcaa cccacaggtt 3841 tgcacatgcc catgagtatg aatgagctta gcaatccatg tttgaaaaaa gaaaatagtg 3901 aattgcggag aactgtgtac ttatgtacgg atgataattt gagaggggct gacatggaca 3961 tagtccatcc tcaagaaaga atgatggaaa gtgactatat tgtgatgccc agaagttctg 4021 taaataacca gccttcaatg aaagaagaaa gcaaaatgaa tattggcatg gaaaccttgc 4081 cacatgaaag gctattgcac tacaaagtaa accctgaatt caatatgaat ccccctgtaa 4141 tggaccagtt caatatgaac ttagagcaac atctcgcacc ccaggaacat atgcagaatt 4201 tgccctttga acctcgcaca gctgtgaaga atttcatggc ctcTGAGTTG GATGATAATG 4261 CAGGACTATC AAGAAGTGAA ACTGGATCAA CGATATCAAT GAGTTCTTTA GAGAGAAGAA 4321 AATCACGATA TTCAGACCTT GACTTTGAGA AGGTCATGCA TACAAGGAAG AGGCATATGG 4381 AACTATTTCA AGAACTAAAT CAGAAATTTC AAACTTTGGA CAGATTTCGG GATATACCAA 4441 ATACAAGCAG TATGGAAAAC CCCGCACCAA ACAAGAATCC ATGGGACACT TTCAAAAACC 4501 CCAGTGAATA CCCGCATTAC ACCACAATCA ATGTCTTAGA CACAGAGGCA AAGGATGCTT 4561 TGGAACTGAG GCCAGCAGAG TGGGAGAAGT GTCTGAATTT GCCTCTGGAT GTGCAAGAGG 4621 GTGACTTTCA AACAGAAGTT GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 4681 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 4741 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGAAA CAGACTACTT 4801 CGTCAAGTGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt