Construct: ORF TRCN0000489517
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021284.1_s317c1
- DNA Barcode:
- AACCAAAAAGCGTTGCCTAATCGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PPARA (5465)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489517
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5465 | PPARA | peroxisome proliferator act... | NM_001001928.3 | 53.5% | 49.5% | (many diffs) |
| 2 | human | 5465 | PPARA | peroxisome proliferator act... | NM_001362872.2 | 53.5% | 49.5% | (many diffs) |
| 3 | human | 5465 | PPARA | peroxisome proliferator act... | NM_001362873.1 | 53.5% | 49.5% | (many diffs) |
| 4 | human | 5465 | PPARA | peroxisome proliferator act... | NM_005036.6 | 53.5% | 49.5% | (many diffs) |
| 5 | human | 5465 | PPARA | peroxisome proliferator act... | XM_005261656.3 | 53.5% | 49.5% | (many diffs) |
| 6 | human | 5465 | PPARA | peroxisome proliferator act... | XM_006724270.3 | 53.5% | 49.5% | (many diffs) |
| 7 | human | 5465 | PPARA | peroxisome proliferator act... | XM_011530239.2 | 53.5% | 49.5% | (many diffs) |
| 8 | human | 5465 | PPARA | peroxisome proliferator act... | XM_011530240.2 | 53.5% | 49.5% | (many diffs) |
| 9 | human | 5465 | PPARA | peroxisome proliferator act... | XM_011530241.2 | 53.5% | 49.5% | (many diffs) |
| 10 | human | 5465 | PPARA | peroxisome proliferator act... | XM_011530242.2 | 53.5% | 49.5% | (many diffs) |
| 11 | human | 5465 | PPARA | peroxisome proliferator act... | XM_011530243.2 | 53.5% | 49.5% | (many diffs) |
| 12 | human | 5465 | PPARA | peroxisome proliferator act... | XR_937870.2 | 50.1% | (many diffs) | |
| 13 | human | 5465 | PPARA | peroxisome proliferator act... | XR_937869.2 | 49.9% | (many diffs) | |
| 14 | human | 5465 | PPARA | peroxisome proliferator act... | XM_011530244.2 | 24.7% | 15.2% | (many diffs) |
| 15 | human | 5465 | PPARA | peroxisome proliferator act... | XM_011530245.2 | 24.7% | 15.2% | (many diffs) |
| 16 | human | 5465 | PPARA | peroxisome proliferator act... | XM_017028839.1 | 24.7% | 15.2% | (many diffs) |
| 17 | human | 5465 | PPARA | peroxisome proliferator act... | XM_024452252.1 | 24.7% | 15.2% | (many diffs) |
| 18 | human | 5465 | PPARA | peroxisome proliferator act... | XR_001755253.1 | 7.4% | (many diffs) | |
| 19 | mouse | 19013 | Ppara | peroxisome proliferator act... | XM_006520624.3 | 44.7% | 45.7% | (many diffs) |
| 20 | mouse | 19013 | Ppara | peroxisome proliferator act... | NM_001113418.1 | 44.6% | 45.6% | (many diffs) |
| 21 | mouse | 19013 | Ppara | peroxisome proliferator act... | NM_011144.6 | 44.6% | 45.6% | (many diffs) |
| 22 | mouse | 19013 | Ppara | peroxisome proliferator act... | XM_006520619.1 | 44.6% | 45.6% | (many diffs) |
| 23 | mouse | 19013 | Ppara | peroxisome proliferator act... | XM_006520620.3 | 44.6% | 45.6% | (many diffs) |
| 24 | mouse | 19013 | Ppara | peroxisome proliferator act... | XM_006520621.3 | 44.6% | 45.6% | (many diffs) |
| 25 | mouse | 19013 | Ppara | peroxisome proliferator act... | XM_006520622.3 | 44.6% | 45.6% | (many diffs) |
| 26 | mouse | 19013 | Ppara | peroxisome proliferator act... | XM_006520623.3 | 44.6% | 45.6% | (many diffs) |
| 27 | mouse | 19013 | Ppara | peroxisome proliferator act... | XM_011245516.2 | 44.6% | 45.6% | (many diffs) |
| 28 | mouse | 19013 | Ppara | peroxisome proliferator act... | XM_011245517.2 | 44.6% | 45.6% | (many diffs) |
| 29 | mouse | 19013 | Ppara | peroxisome proliferator act... | XM_011245519.2 | 44.6% | 45.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 846
- ORF length:
- 774
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggtggac acggaaagcc cactctgccc cctctcccca ctcgaggccg 121 gcgatctaga gagcccgtta tctgaagagt tcctgcaaga aatgggaaac atccaagaga 181 tttcgcaatc catcggcgag gatagttctg gaagctttgg ctttacggaa taccagtatt 241 taggaagctg tcctggctca gatggctcgg tcatcacgga cacgctttca ccagcttcga 301 gcccctcctc ggtgacttat cctgtggtcc ccggcagcgt ggacgagtct cccagtggag 361 cattgaacat cgaatgtaga atctgcgggg acaaggccTC AGGCTATCAT TACGGAGTCC 421 ACGCGTGTGA AGGCTGCAAG GGCTTCTTTC GGCGAACGAT TCGACTCAAG CTGGTGTATG 481 ACAAGTGCGA CCGCAGCTGC AAGATCCAGA AAAAGAACAG AAACAAATGC CAGTATTGTC 541 GATTTCACAA GTGCCTTTCT GTCGGGATGT CACACAACGC GATTCGTTTT GGACGAATGC 601 CAAGATCTGA GAAAGCAAAA CTGAAAGCAG AAATTCTTAC CTGTGAACAT GACATAGAAG 661 ATTCTGAAAC TGCAGATCTC AAATCTCTGG CCAAGAGAAT CTACGAGGCC TACTTGAAGA 721 ACTTCAACAT GAACAAGGTC AAAGCCCGGG TCATCCTCTC AGGAAAGGCC AGTAACAATC 781 CAGTAGGTGT TTGCGGCTGT TCTGGGTTCT CTTGGCAACA TGGAACCAGT GTCGTAGAGG 841 ACGATTAGAA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 901 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 961 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAACCAA AAAGCGTTGC CTAATCGTAC 1021 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt