Construct: ORF TRCN0000489524
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020466.1_s317c1
- DNA Barcode:
- GTGAGGACTTACTTATACAACCCC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DMPK (1760)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489524
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1760 | DMPK | DM1 protein kinase | NM_004409.5 | 99.7% | 88.1% | 1648_1651delCTAG |
| 2 | human | 1760 | DMPK | DM1 protein kinase | NM_001081560.3 | 98.9% | 87.4% | 1130_1131insGGTGAGCGGGGGCGG;1633_1636delCTAG |
| 3 | human | 1760 | DMPK | DM1 protein kinase | NM_001081562.3 | 98.8% | 98.8% | 1130_1131insGGTGAGCGGGGGCGG;1869_1875delTGAACCC |
| 4 | human | 1760 | DMPK | DM1 protein kinase | NM_001288764.2 | 95.8% | 76.9% | 1_19del;179_237del;1726_1729delCTAG |
| 5 | human | 1760 | DMPK | DM1 protein kinase | NM_001081563.2 | 90.4% | 78.9% | (many diffs) |
| 6 | human | 1760 | DMPK | DM1 protein kinase | NM_001288765.1 | 85.5% | 85.5% | 0_1ins267;1617_1623delTGAACCC |
| 7 | human | 1760 | DMPK | DM1 protein kinase | NM_001288766.2 | 84.4% | 84% | 1130_1131insGGTGAGCGGGGGCGG;1584_1585insGCTGTCACGGG;1590_1591ins267 |
| 8 | mouse | 13400 | Dmpk | dystrophia myotonica-protei... | NM_032418.2 | 86% | 76.7% | (many diffs) |
| 9 | mouse | 13400 | Dmpk | dystrophia myotonica-protei... | XM_011250433.2 | 85.9% | 86.2% | (many diffs) |
| 10 | mouse | 13400 | Dmpk | dystrophia myotonica-protei... | XM_006539519.3 | 85.2% | 75.9% | (many diffs) |
| 11 | mouse | 13400 | Dmpk | dystrophia myotonica-protei... | XM_006539518.3 | 85.1% | 85.4% | (many diffs) |
| 12 | mouse | 13400 | Dmpk | dystrophia myotonica-protei... | XM_006539520.3 | 83.8% | 75.8% | (many diffs) |
| 13 | mouse | 13400 | Dmpk | dystrophia myotonica-protei... | XM_006539516.3 | 83.1% | 73.6% | (many diffs) |
| 14 | mouse | 13400 | Dmpk | dystrophia myotonica-protei... | NM_001190490.1 | 82.5% | 73.2% | (many diffs) |
| 15 | mouse | 13400 | Dmpk | dystrophia myotonica-protei... | XM_006539517.3 | 82.3% | 72.9% | (many diffs) |
| 16 | mouse | 13400 | Dmpk | dystrophia myotonica-protei... | XM_006539521.3 | 81.7% | 78.8% | (many diffs) |
| 17 | mouse | 13400 | Dmpk | dystrophia myotonica-protei... | XM_006539522.2 | 77.7% | 76.2% | (many diffs) |
| 18 | mouse | 13400 | Dmpk | dystrophia myotonica-protei... | NM_001190491.1 | 73.1% | 74.3% | (many diffs) |
| 19 | mouse | 13400 | Dmpk | dystrophia myotonica-protei... | XR_001785471.1 | 58.3% | (many diffs) | |
| 20 | mouse | 13400 | Dmpk | dystrophia myotonica-protei... | XR_001785470.1 | 58.2% | (many diffs) | |
| 21 | mouse | 13400 | Dmpk | dystrophia myotonica-protei... | XR_001785474.1 | 57.6% | (many diffs) | |
| 22 | mouse | 13400 | Dmpk | dystrophia myotonica-protei... | XR_001785472.1 | 56.6% | (many diffs) | |
| 23 | mouse | 13400 | Dmpk | dystrophia myotonica-protei... | XR_391041.2 | 56.1% | (many diffs) | |
| 24 | mouse | 13400 | Dmpk | dystrophia myotonica-protei... | NR_126095.1 | 55.9% | (many diffs) | |
| 25 | mouse | 13400 | Dmpk | dystrophia myotonica-protei... | XR_001785473.1 | 53.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1956
- ORF length:
- 1884
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtcagcc gaggtgcggc tgaggcggct ccagcagctg gtgttggacc 121 cgggcttcct ggggctggag cccctgctcg accttctcct gggcgtccac caggagctgg 181 gcgcctccga actggcccag gacaagtacg tggccgactt cttgcagtgg gcggagccca 241 tcgtggtgag gcttaaggag gtccgactgc agagggacga cttcgagatt ctgaaggtga 301 tcggacgcgg ggcgttcagc gaggtagcgg tagtgaagat gaagcagacg ggccaggtgt 361 atgccatgaa gatcatgaac aagtgggaca tgctgaagag gggcgaggtg tcgtgcttcc 421 gtgaggagag ggacgtgttg gtgaatgggg accggcggtg gatcacgcag ctgcacttcg 481 ccttccagga tgagaactac ctgtacctgg tcatggagta ttacgtgggc ggggacctgc 541 tgacactgct gagcaagttt ggggagcgga ttccggccga gatggcgcgc ttctacctgg 601 cggagattgt catggccata gactcggtgc accggcttgg ctacgtgcac agggacatca 661 aacccgacaa catcctgctg gaccgctgtg gccacatccg cctggccgac ttcggctctt 721 gcctcaagct gcgggcagat ggaacggtgc ggtcgctggt ggctgtgggc accccagact 781 acctgtcccc cgagatcctg caggctgtgg gcggtgggcc tgggacaggc agctacgggc 841 ccgagtgtga ctggtgggcg ctgggtgtat tcgcctatga aatgttctat gggcagacgc 901 ccttctacgc ggattccacg gcggagacct atggcaagat cgtccactac aaggagcacc 961 tctctctgcc gctggtggac gaaggggtcc ctgaggaggc tcgagacttc attcagcggt 1021 tgctgtgtcc cccggagaca cggctgggcc ggggtggagc aggcgacttc cggacacatc 1081 ccttcttctt tggcctcgac tgggatggtc tccgggacag cgtgcccccc tttacaccgg 1141 atttcgaagg tgccaccgac acatgcaact tcgacttggt ggaggacggg ctcactgcca 1201 tggtgagcgg gggcggggag acactgtcgg acattcggga aggtgcgccg ctaggggtcc 1261 acctgccttt tgtgggctac tcctactcct gcatggccct cagggacagt gaggtcccag 1321 gccccacacc catggaactg gaggccgagc agctgcttga gccacacgtg caagcgccca 1381 gcctggagcc ctcggtgtcc ccacaggatg aaacagctga agtggcagtt ccagcggctg 1441 tccctgcggc agaggctgag gccgaggtga cgctgcggga gctccaggaa gccctggagg 1501 aggaggtgct cacccggcag agcctGAGCC GGGAGATGGA GGCCATCCGC ACGGACAACC 1561 AGAACTTCGC CAGTCAACTA CGCGAGGCAG AGGCTCGGAA CCGGGACCTA GAGGCACACG 1621 TCCGGCAGTT GCAGGAGCGG ATGGAGTTGC TGCAGGCAGA GGGAGCCACA GCTGTCACGG 1681 GGGTCCCCAG TCCCCGGGCC ACGGATCCAC CTTCCCATAT GGCCCCCCGG CCGTGGCTGT 1741 GGGCCAGTGC CCGCTGGTGG GGCCAGGCCC CATGCACCGC CGCCACCTGC TGCTCCCTGC 1801 CAGGGTCCCT AGGCCTGGCC TATCGGAGGC GCTTTCCCTG CTCCTGTTCG CCGTTGTTCT 1861 GTCTCGTGCC GCCGCCCTGG GCTGCATTGG GTTGGTGGCC CACGCCGGCC AACTCACCGC 1921 AGTCTGGCGC CGCCCAGGAG CCGCCCGCGC TCCCTAGGAC CCAGCTTTCT TGTACAAAGT 1981 GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG 2041 AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 2101 CGAGTGAGGA CTTACTTATA CAACCCCACG CGTTAAGTCg acaatcaacc tctggattac 2161 aaaatttgtg aaagatt