Construct: ORF TRCN0000489525
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020364.1_s317c1
- DNA Barcode:
- CCCTGATCTCATCTGTTTTTCAGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- STK33 (65975)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489525
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001289061.2 | 99.6% | 99.4% | (many diffs) |
| 2 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352387.2 | 99.6% | 99.4% | (many diffs) |
| 3 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352388.2 | 99.6% | 99.4% | (many diffs) |
| 4 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352389.2 | 99.6% | 99.4% | (many diffs) |
| 5 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352390.2 | 99.6% | 99.4% | (many diffs) |
| 6 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352391.2 | 99.6% | 99.4% | (many diffs) |
| 7 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352392.2 | 99.6% | 99.4% | (many diffs) |
| 8 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352393.2 | 99.6% | 99.4% | (many diffs) |
| 9 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_030906.4 | 99.6% | 99.4% | (many diffs) |
| 10 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_011520290.2 | 99.6% | 99.4% | (many diffs) |
| 11 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_011520295.2 | 99.6% | 99.4% | (many diffs) |
| 12 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018144.2 | 99.6% | 99.4% | (many diffs) |
| 13 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018145.1 | 99.6% | 99.4% | (many diffs) |
| 14 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018146.1 | 99.6% | 99.4% | (many diffs) |
| 15 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018147.1 | 99.6% | 99.4% | (many diffs) |
| 16 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018148.1 | 99.6% | 99.4% | (many diffs) |
| 17 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018152.1 | 99.6% | 99.4% | (many diffs) |
| 18 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018153.2 | 99.6% | 99.4% | (many diffs) |
| 19 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018154.1 | 99.6% | 99.4% | (many diffs) |
| 20 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018155.2 | 99.6% | 99.4% | (many diffs) |
| 21 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018156.1 | 99.6% | 99.4% | (many diffs) |
| 22 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001289058.2 | 91.6% | 91.4% | (many diffs) |
| 23 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352394.2 | 91.6% | 91.4% | (many diffs) |
| 24 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352395.2 | 91.6% | 91.4% | (many diffs) |
| 25 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_011520297.2 | 91.6% | 91.4% | (many diffs) |
| 26 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018157.1 | 91.6% | 91.4% | (many diffs) |
| 27 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352399.2 | 86.9% | 86.9% | (many diffs) |
| 28 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018159.1 | 86.9% | 86.9% | (many diffs) |
| 29 | human | 65975 | STK33 | serine/threonine kinase 33 | XM_017018161.1 | 78.3% | 72.5% | (many diffs) |
| 30 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352396.2 | 70.4% | 64.7% | (many diffs) |
| 31 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352397.2 | 70.4% | 64.7% | (many diffs) |
| 32 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001352398.2 | 70.4% | 64.7% | (many diffs) |
| 33 | human | 65975 | STK33 | serine/threonine kinase 33 | NM_001289059.2 | 63.3% | 63% | (many diffs) |
| 34 | human | 65975 | STK33 | serine/threonine kinase 33 | XR_001747938.1 | 34.6% | (many diffs) | |
| 35 | human | 65975 | STK33 | serine/threonine kinase 33 | XR_001747939.1 | 25.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1614
- ORF length:
- 1542
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggctgat agtggcttag ataaaaaatc cacaaaatgc cccgactgtt 121 catctgcttc tcagaaagat gtactttgtg tatgttccag caaaacaagg gttcctccag 181 ttttggtggt ggaaatgtca cagacatcaa gcattggtag tgcagaatct ttaatttcac 241 tggagagaaa aaaagaaaaa aatatcaaca gagatataac ctccaggaaa gatttgccct 301 caagaacctc aaatgtagag agaaaagcat ctcagcaaca atggggtcgg ggcaacttta 361 cagaaggaaa agttcctcac ataaggattg agaatggagc tgctattgag gaaatctata 421 cctttggaag aatattggga aaagggagct ttggaatagt cattgaagct acagacaagg 481 aaacagaaac gaagtgggca attaaaaaag tgaacaaaga aaaggctgga agctccgctg 541 tgaagttact tgaacgagag gtgaacattc tgaaaagtgt aaaacatgaa cacatcatac 601 atctggaaca agtatttgaa acgccaaaga aaatgtacct tgtgatggag ctttgtgagg 661 atggagaact caaagaaatt ctggatagga aagggcattt ctcagagaat gagacaaggt 721 ggatcattca aagtctcgca tcagctatag catatcttca caataatgat attgtacata 781 gggatctgaa actggaaaat ataatggtta aaagcagtct tattgatgat aacaatgaaa 841 taaacttaaa cataaaggtg actgattttg gcttagcggt gaagaagcaa agtaggagtg 901 aagccatgct gcaggccaca tgtgggactc ctatctatat ggcccctgaa gttatcagtg 961 cccacgacta tagccagcag tgtgacattt ggagcatagg cgtcgtaatg tacatgttat 1021 tacgtggaga accacccttt ttggcaagct cagaagagaa gctttttgag ttaataagaa 1081 aaggagaact acattttgaa aatgcagtct ggaattccat aagtgactgt gctaaaagtg 1141 ttttgaaaca acttatgaaa gtagatcctg ctcacagaat cacagctaag gaactactag 1201 ataaccagtg gttaacaggC AATAAACTTT CTTCGGTGAG ACCAACCAAT GTATTAGAGA 1261 TGATGAAGGA ATGGAAAAAT AACCCAGAAA GTGTTGAGGA AAACACAACA GAAGAGAAGA 1321 ATAAGCCGTC CACTGAAGAA AAGTTGAAAA GTTACCAACC CTGGGGAAAT GTCCCTGAGA 1381 CCAATTACAC TTCAGATGAA GAGGAGGAAA AACAGTCTAC TACTTATGAA AAGCAATTTC 1441 CTGCAACCAG TAAGGACAAC TTTGATATGT GCAGTTCAAG TTTCACATCT AGCAAACTCC 1501 TTCCAGCTGA AATCAAGGGA GAAATGGAGA AAACCCCTGT GACTCCAAGC CAAGGAACAG 1561 CAACCAAGTA CCCTGCTAAA TCCGGCGCCC TGTCCAGAAC CAAAAAGAAA CTCTAGGACC 1621 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1681 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1741 ATATATCTTG TGGAAAGGAC GACCCTGATC TCATCTGTTT TTCAGTACGC GTTAAGTCga 1801 caatcaacct ctggattaca aaatttgtga aagatt