Construct: ORF TRCN0000489527
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019175.1_s317c1
- DNA Barcode:
- AGTACACTGCCAGCCCCGCGCCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTH1R (5745)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489527
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | NM_000316.3 | 99.8% | 99.8% | 1389T>C;1779_1780insG |
| 2 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | NM_001184744.1 | 99.8% | 99.8% | 1389T>C;1779_1780insG |
| 3 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_017006933.1 | 99.8% | 99.8% | 1389T>C;1779_1780insG |
| 4 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_011533967.3 | 97.7% | 97.6% | 175_213del;1428T>C;1818_1819insG |
| 5 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_017006932.2 | 97.7% | 97.6% | 175_213del;1428T>C;1818_1819insG |
| 6 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_011533968.2 | 95% | 92.3% | (many diffs) |
| 7 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_005265344.3 | 94.6% | 94.6% | 0_1ins93;1296T>C;1686_1687insG |
| 8 | human | 5745 | PTH1R | parathyroid hormone 1 receptor | XM_017006934.1 | 82.9% | 68.5% | (many diffs) |
| 9 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | NM_001083935.1 | 86.4% | 90% | (many diffs) |
| 10 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | NM_001083936.1 | 86.4% | 90% | (many diffs) |
| 11 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | NM_011199.2 | 86.4% | 90% | (many diffs) |
| 12 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | XM_006511988.3 | 84.1% | 85.5% | (many diffs) |
| 13 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | XM_017313215.1 | 84.1% | 85.5% | (many diffs) |
| 14 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | XM_006511987.2 | 82.7% | 84% | (many diffs) |
| 15 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | XM_017313214.1 | 78.7% | 78.7% | (many diffs) |
| 16 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | XM_006511985.2 | 69.7% | 69.8% | (many diffs) |
| 17 | mouse | 19228 | Pth1r | parathyroid hormone 1 receptor | XM_011242942.1 | 55.9% | 55.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1857
- ORF length:
- 1782
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgggg accgcccgga tcgcacccgg cctggcgctc ctgctctgct 121 gccccgtgct cagctccgcg tacgcgctgg tggatgcaga tgacgtcatg actaaagagg 181 aacagatctt cctgctgcac cgtgctcagg cccagtgcga aaaacggctc aaggaggtcc 241 tgcagaggcc agccagcata atggaatcag acaagggatg gacatctgcg tccacatcag 301 ggaagcccag gaaagataag gcatctggga agctctaccc tgagtctgag gaggacaagg 361 aggcacccac tggcagcagg taccgagggc gcccctgtct gccggaatgg gaccacatcc 421 tgtgctggcc gctgggggca ccaggtgagg tggtggctgt gccctgtccg gactacattt 481 atgacttcaa tcacaaaggc catgcctacc gacgctgtga ccgcaatggc agctgggagc 541 tggtgcctgg gcacaacagg acgtgggcca actacagcga gtgtgtcaaa tttctcacca 601 atgagactcg tgaacgggag gtgtttgacc gcctgggcat gatttacacc gtgggctact 661 ccgtgtccct ggcgtccctc accgtagctg tgctcatcct ggcctacttt aggcggctgc 721 actgcacgcg caactacatc cacatgcacc tgttcctgtc cttcatgctg cgcgccgtga 781 gcatcttcgt caaggacgct gtgctctact ctggcgccac gcttgatgag gctgagcgcc 841 tcaccgagga ggagctgcgc gccatcgccc aggcgccccc gccgcctgcc accgccgctg 901 ccggctacgc gggctgcagg gtggctgtga ccttcttcct ttacttcctg gccaccaact 961 actactggat tctggtggag gggctgtacc tgcacagcct catcttcatg gccttcttct 1021 cagagaagaa gtacctgtgg ggcttcacag tcttcggctg gggtctgccc gctgtcttcg 1081 tggctgtgtg ggtcagtgtc agagctaccc tggccaacac cgggtgctgg gacttgagct 1141 ccgggaacaa aaagtggatc atccaggtgc ccatcctggc ctccattgtg ctcaacttca 1201 tcctcttcat caatatcgtc cgggtgctcg ccaccaagct gcgggagacc aacgccggcc 1261 ggtgtgacac acggcagcag taccggaagc tgctcaaatc cacgctggtg ctcatgcccc 1321 tctttggcgt ccactacatt gtcttcatgg ccacaccata caccgaggtc tcagggacgc 1381 tctggcaagt ccagatgcac tatgagatgc tcttcaactc cttccaggga ttttttgtcg 1441 caatcatata ctgtttctgc aacggcgagg tacaagctga gatcaagaaa tcttggagcc 1501 gctggacact GGCACTGGAC TTCAAGCGAA AGGCACGCAG CGGGAGCAGC AGCTATAGCT 1561 ACGGCCCCAT GGTGTCCCAC ACAAGTGTGA CCAATGTCGG CCCCCGTGTG GGACTCGGCC 1621 TGCCCCTCAG CCCCCGCCTA CTGCCCACTG CCACCACCAA CGGCCACCCT CAGCTGCCTG 1681 GCCATGCCAA GCCAGGGACC CCAGCCCTGG AGACCCTCGA GACCACACCA CCTGCCATGG 1741 CTGCTCCCAA GGACGATGGG TTCCTCAACG GCTCCTGCTC AGGCCTGGAC GAGGAGGCCT 1801 CTGGGCCTGA GCGGCCACCT GCCCTGCTAC AGGAAGAGTG GGAGACAGTC ATGGACCCAG 1861 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1921 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1981 TATCTTGTGG AAAGGACGAA GTACACTGCC AGCCCCGCGC CTGACGCGTT AAGTCgacaa 2041 tcaacctctg gattacaaaa tttgtgaaag att